菌毛形成

Main results were presented as follows:Among 21 bacteria strains, 3 strains induced conidia of the pathogen to produce swollen cells on the sites of the appressorium differentation.

本研究筛选到四类对稻瘟病菌附着胞形成具有不同抑制机制的拮抗细菌，并对h23菌株的抗菌活性进行详细分析；同时还测定了毛壳菌对稻瘟病菌附着胞的抑制作用。

Seedlings in these 11 nurseries. This study primaryly confirmed symbiotic fungi with Pinus Tabulaeformis Carr. belonging to 3 genus of basidiomycete based on the morphological and anotamical characteristics, they were Suillus Mich.ex Gray, Russula Gray and Tomentella Pat.; Suillus Mich.ex Gray was the predominated fugus of Pinus Tabulaeformis Carr.

本研究根据外生菌根形态和解剖结构特征，初步确定与本次调查油松形成外生菌根的菌根真菌为担子菌，分属3个属，他们分别是乳牛肝菌属(Suillus Mich.ex Gray)、红菇属和毛革菌属Tomentella Pat。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

The effects of microbial inoculant on humification and humus qualityare studied.The results show that inoculating Phanerochaete chrysosporiumin second fermentation phase of composting could promote the formation ofhumus,increase the humus content,and reduce the humification time by 7days.

通过考察接种微生物对堆肥腐熟程度及成品腐殖质含量的影响，发现在堆肥二次发酵阶段接种黄孢原毛平革菌能优化控制腐殖质的形成，提高了总腐殖质含量，增强了堆肥成品的腐熟程度，并使堆肥腐熟时间缩短了7d左右。

The IV style pilus and the twitching motility mediated by the IV style pilus play an absolutely necessary role in the early stage of the pseudomonsa aeruginosa biofilm forming.

IV型菌毛及它所介导的蹭动在铜绿假单胞菌生物被膜形成的早期起着必不可少的作用。

Eleven subcloned DNA fragments from the 5′- upstream region of lipA, lipC and lipF of Phanerochaete chrysosporium were assayed by using the gel mobility shift assay. The total proteins extracted from P.chrysosporium mycelia grown in Kirk medium and natural fir wood chip were used to identify the segments in these 11 DNA fragments which are controlled by some regulatory proteins.

用DNA-蛋白质体外结合实验和凝胶迁移率变动分析技术筛选黄孢原毛平革菌木质素过氧化物酶基因lipA、lipC、lipF的5′-端调控区内能与该菌在木质素降解条件下形成的蛋白特异结合的顺式作用元件。

Result The highest level of growth factor 61.1μg/L,was produced by trichophyton rubrum(turbidimetry 4.5×10 10 ),and the strongest effect was at the point of double diluted filtrarate,cell index reached 2800 cpm/dis...

结果 红色毛癣菌分泌EFG最高 6 1 。1 μg/L(浊度比 4 。5× 1 0 10 )，对倍稀释液对培养的原代人类角质形成细胞作用最强，角质形成细胞标记指数可达 2 80 0cpm/盘。

Objective To establish a rapid method for detecting Enteropathogenic Escherichia coli EPEC in meat and meat product. Methods Based on attaching and effacing lesion and bundle orming pili of EPEC, two pairs of primers were designed.

以 EPEC 特异的微绒毛粘连基因和菌毛束形成编码基因作为模板，设计两对引物对肉及肉制品中的 EPEC 进行特异性扩增。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。