菌毛

Results: The autofluorescence of M. furfur and M. japonica standard trainsrespectively in 4th and 6th day are stronger than that in other days, andautofluorescence of colonies become weaker in an hour. Theautofluorescence of Malassezia colonies put in 3% glutaraldehyde, pH4.0HCL, 1280μg/mL and 64μg/mL fluconazol solution, 0.3% methylene blueseparately get stronger, and that in 10% KOH get stronger at beginning thenbecome weaker 2 days later. C. albicans, S. schenchii, T. rubrum, T.tonsurans, A. terreus, A. fumigatus cultured on SDA all haveautofluorescence, and which are weaker than that of Malassezia.

结果：糠秕马拉色菌和日本马拉色菌2株标准株的自发荧光强度分别在培养第4和8天时最强，菌落的自发荧光在1小时内逐渐减弱；3％戊二醛溶液、pH4.0盐酸溶液、1280μg／mL和64μg／mL氟康唑溶液、3％美蓝溶液处理后马拉色菌自发荧光都增强，10％氢氧化钾溶液中的马拉色菌在开始时荧光增强，2天后减弱；白念珠菌、申克孢子丝菌、红色毛癣菌、断发毛癣菌、土曲霉、烟曲霉等在培养条件下有自发荧光，但都较马拉色菌弱。

To explore the possibility of early and rapid diagnosis of mycosisby detecting the fungal autofluorescence. Methods: To culture M. furfur and M. japonica standard strains on Dixonmedium at 32℃, and to study the autofluorescence of colonies in 2nd, 4th, 6th, 8th, 10th day under Laser Scanning Confocal Microscopy (LeicaTCS-SP2 LSCM). To study the variation of autofluorescence whenMalassezia colonies were put in 3% glutaraldehyde, pH4.0 HCL, 10% KOH, fluconazol solution, 0.3% methylene blue separately. To detect theautofluorescence of C. albicans, S. schenchii, T. rubrum, T. tonsurans, A.terreus, A. fumigatus cultured on Sabouraud dextrose agar.

选用糠秕马拉色菌和日本马拉色菌2株标准菌，接种在Dixon培养基32℃培养，分别在培养的第2、4、6、8、10天挑取菌落放在TCS-SP2型激光扫描共聚焦显微镜(Leica TCS-SP2 LSCM)下观察、测定其自发荧光；分别用3％戊二醛溶液、pH4.0盐酸溶液、10％氢氧化钾溶液、1280μg／mL和64μg／mL氟康唑溶液、0.3％美蓝溶液处理后观察马拉色菌自发荧光的变化；选择白念珠菌、申克孢子丝菌、红色毛癣菌、断发毛癣菌、土曲霉、烟曲霉等用沙堡葡萄糖琼脂培养后观察其自发荧光的特点。

This paper aimed to investigate the antagonism between Chaetomium globosum and Rhizoctonia solani with GFP as reporter gene.

为了从分子生物学水平研究球毛壳菌与立枯丝核菌的拮抗机制，选用GFP作为报告基因对球毛壳菌进行标记。

There was big difference among the 22 different materials in the disease resistance. Translocation lines Pm97033 showed the highest resistance in reaction than others. The percentage of diseased root and severed level were 11.3% and 5.4%; substitution lines Wan7107 showed medium resistance to the fungus, the percentage of diseased root and severity level were 21.4% and 10.6%; F5 generation of common wheat with Till showed higher resistance to take-all fungus than the PS progenies of common wheat with Aegilops tauschii or the other common wheat varieties.

菌饼＋菌粒法对宁春4号的致病力明显高于菌饼法和菌粒法，其病根率、严重度和病茎率分别达到100.0%，57.3%和28.6%。22个遗传背景不同的小麦材料间抗病性差异较大，其中小麦－簇毛麦易位系Pm97033的抗病性最强，病根率和严重度分别为11.3%和5.4%；代换系Wan7107次之，病根率和严重度分别为21.4%和10.6%；硬粒小麦－簇毛麦双二倍体(TH1)与普通小麦品种杂交后代的抗病性强于粗山羊草与普通小麦品种杂交后代。

Species diversity of Lachnum from Huangshan Moutains Anhui China weresurveyed, Nine taxa were reported. Tissue isolation was performed on 97 discomycetes specimens fromHuangshan Mountains, 33 filament products were obtained.

我国安徽黄山等地物种丰富，但是对粒毛盘菌属物种多样性的报道却相对较少，本研究对黄山等地粒毛盘菌属的物种多样性进行了分类学研究，报道了黄山粒毛盘菌属9个分类单元。

Funalia gallica, Lenzites tricolor, Polyporellus brumalis, Pseudotrametes gibbosa, Pycnoporus sanguineus, tested their degrading ability to david poplar wood lignin, measured the lignin content change of david poplar wood after a period of time degraded by 6 wood rot fungi, which is the first study on the biodegradation mechanism of lignin by wood white rot fungi.

本项研究选择了火木层孔菌及另外5种木材分解能力较强的阔叶树上的白腐菌：粗毛盖菌、三色革裥菌、冬拟多孔菌、偏肿拟栓菌和血红密孔菌，研究了它们对山杨木材木质素的分解能力，测定了经6种白腐菌分解一定时期的山杨木材木质素的含量，作为木材白腐菌对山杨木材木质素生物降解机制的初步研究，旨在为山杨木材生物制浆造纸提供应用基础理论研究，同时也可为木质素合理的生物转化为有用的化学品、生物漂白、酶处理防止机械浆的返黄、废水治理、纤维素酶解糖化的微生物前处理等提供相关的借鉴研究，以期在生产实践中减轻环境污染并充分利用木质素资源。

The results indicated that the strain was Mracemosus fresenius which belonged to Racemousus, Muco, Mucoraceae, Mucorales, Phycomycetes in Fungi. Its growth temperature was under 37℃, and the optimal temperature was determined to 28℃.

结果表明，该菌株为真菌门、藻菌纲、毛霉目、毛霉科、毛霉属、总状枝毛霉组中的总状枝毛霉。

Objective To observe the unique DNA profile and the relationship between DNA profile and phenotype of Trichophyton rubrum, and establish an effective molecular method to identify T. rubrum.

目的用PCR方法扩增红色毛癣菌和须癣毛癣菌临床分离株的DNA，观察PCR指纹的差异以及基因型与传统表型的关系，建立一种红色毛癣菌分子生物学鉴定方法。

Methods: Concentration gradient method was used to detect the minimal inhibitory concentrations of DNA-2E on trichophyton rubrum and beard-like trichophyton.

采用浓度梯度法研究癣清(DNA-2E)对红色毛癣菌、须状毛癣菌的最低抑菌浓度。

Objective To observe the unique DNA profile and the relationship between DNA profile and pheno type of Trichophyton rubrum , and establish an effective molecular biologic method to identify T. Rubrum.

目的 用聚合酶链反应扩增红色毛癣菌和须癣毛癣菌临床分离株的DNA，观察PCR指纹的差异以及基因型与传统表型的关系，建立一种红色毛癣菌分子生物学鉴定方法。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。