菌根

Chinese Cinnamaldehyde which also named Cinnamaldehyde alias, is the main component of volatile oil which obtained by steam distillation from dry skin and cinnamon bark of lauraceae plants . It is researched as a root canal sterilization drug in recent years, it can seriously undermine the bacterial cell surface structure, and thus has a wider antibacterial spectrum and strong antibacterial activity; gallnut hundred, also known as worms, it is a kind of gall that be formatted by Schlechtendalia species of insects including Schlechtendalia and times aphid eggs in their parasitic plants Anacardiaceae Rhus chinensis, Green bran Yang or red bran Yang, the main ingredient for gallnut tannin, can solidification microbial protoplasts and a variety of body enzymes, a wide range of bacteria, fungi, yeasts are inhibited.

中药桂皮醛别名肉桂醛，是樟科植物肉桂的干皮及树皮经水蒸气蒸馏得到的挥发油中的主要成分，是近年来研究较多的根管消毒药物，它能够严重破坏细菌细胞表面结构，从而具有较广的抗菌谱和较强的抗菌活性；五倍子又名百虫，是倍蚜种昆虫角倍蚜或倍蛋蚜在其寄生漆树科植物盐肤木、青麸杨或红麸杨等树上形成的虫瘿，其主要成分为五倍子鞣质，能够凝固微生物体内的原生质及多种酶，对多种细菌、真菌、酵母菌都有明显的抑制作用。

Effect of tenuazonic acid on micronucleus and karyokinesis of Vicia faba root tip cells was investigated to evaluate its cytogenetic toxicity.

利用蚕豆根尖为材料，研究了链格孢菌毒素细交链孢菌酮酸对细胞微核和有丝分裂的影响，以评价其细胞遗传毒性。

The results showed that vanillin had the inhibition effect intensively, the minimum inhibition concentration of vanillin against E. coil was 0.23%, Bacillus subtilis was 0.26%, Saccharomyces was 0.14%, and Aspergillus was 0.14% and Mucor rouxii was 0.03%.

结果表明，香兰素有较强的抑菌性，对大肠埃希菌的最低抑制浓度为0.23%，枯草芽胞杆菌为0.26%，酵母菌为0.14%，黑根霉和毛霉分别为0.14%和0.03%。

The author makes an amendment of the name of Reeds Fungi on the basis of the archaian document as well as of the Modern Mycology knowledge and fieldwork.Analysing the reeds fungi in terms of the principles and methods of the Culture Anthropology,the author makes a discovery that it originated from the penis cult.

作者在整理古代文献的基础上，结合现代菌物学知识和野外考察，对雚菌的现代菌物名称进行了考订；并运用文化人类学的原理和方法进行分析，揭示出雚菌的名称是来源于古代男根崇拜。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

Results The rates of bacteria detection and positive rates from infected root canals were high. Anaerobia and aerobia were sensitive to different kinds of antibiotics.

结果感染根管的细菌检出率、阳性率均很高，厌氧菌、需氧菌对各种抗菌药物敏感。

This paper has researched on the metabolic mechanism and the method of purification of an antibiotic substance produced by Rhizopus chinesis 12th.

本论文对中国根霉12~#菌株产生的抑菌活性物质的代谢机理及其分离纯化方法进行了研究。

This paper at first separated the protoplast from rhizopus and yeast respectively, and then merged the protoplasts of them.

利用分离的根霉、假丝酵母菌种，首先各自进行菌体活化，原生质体制备，然后将两菌进行杂交融合。

One cellulase-production strain TC1 was screened. It was identified as Rhizopus sp. based on identification method of fungi.

筛选得到1株纤维素酶产生菌TC1，根据真菌的分类鉴定方法，鉴定为根霉属菌(Rhizopus sp。)。

Mainly using protein - film voltammetric technique, we have achieved direct electrochemical response of lots of proteins such as hemoglobin, myoglobin, cytocrome c, cytocrome b5, horseradish peroxiase, GOD ,Vitreoscillia sp. haemoglobin, etc., at a series of protein membrane modified electrodes, for instance, protein/kieselgubr, protein/polyethylene glycol, protein/polyethyleneimine, protein/phosphatidylcholine, protein/DNA, protein/montmorillonite/polyvinyl alcohol, protein/silver nanoparticle/ cysteamine membrane modified electrode, etc., which are prepared by casting, incorperating or layer by layer self-assembly methods. Electrochemical behavior of these proteins in the films have been studied, and their structural and conformational changes have also been examined with spectrometric techniques.

三年来，在基金委的支持下，我们以蛋白膜伏安法这一新技术作为主要的研究手段，并结合其他生物、化学技术，研究了血红蛋白、肌红蛋白、细胞色素C、细胞色素b5、辣根过氧化物酶、葡萄糖氧化酶以及好氧革兰式阴性菌透明颤菌中的血红素类蛋白等多种蛋白质在以涂布和自组装方法制成的蛋白质/硅藻土膜、蛋白质/聚乙二醇膜、蛋白质/卵磷脂膜、蛋白质/聚乙烯亚胺膜、蛋白质/蒙托石/聚乙烯醇自组装膜以及蛋白质/银胶/半胱胺自组装膜等修饰电极上的直接电化学行为，结合各种光谱技术，对蛋白质在膜中的构象进行表征，探索其电子传递机理。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。