菌体

Rabbits were inoculated with formaldehyde-inactivated bacteria suspensions of Actinobacillus pleuropneumoniaestrains of 12 serotypes with bee glue as an adjuvant.to prepare a set of monospecific antisera against each serotypes of APR.

将猪传染性胸膜肺炎放线杆菌(Actinobacillus pleuropneumoniae APP)全部12个血清型的国际参考菌株接种培养基大量培养，回收菌体、甲醛灭活后加入蜂胶佐剂制成疫苗，分别免疫健康的试验用白兔，获得了针对APP菌单一血清型的抗血清。

In the culture phase, a great deal of protease, lipase,α_amylase and diastase were accumulated on the skin of beancurd. After 36 h, the thallus began to decay and the activity of all these enzymes began to decline.

培菌阶段在豆腐坯表面积累了大量的蛋白酶、脂肪酶、α_淀粉酶及糖化酶，培菌36 h后菌体开始老化，酶活有所下降。

Selected the best cultivate condition by cultivated on four different media under different conditions; Observated morphology perperties; Studied on biology perperties by germ tube testing, Carbohydrate fermentation testing, Tween testing and Esculin dissociation; lysis testing.

选择四种不同的培养基分别在不同温度条件下培养，选出最优培养条件；通过光学显微镜、电子显微镜观察菌落及菌体的形态学特点；通过芽管试验、碳水化合物发酵试验、碳水化合物同化试验、沙堡培养基生长试验、过氧化氢酶试验、七叶苷分解试验、吐温试验研究该菌的生理、生化学特征；应用PCR技术通过特异性的引物扩增得到菌属特异性的片段，最后测定扩增片段的核苷酸序列，从而进行菌种的分析、鉴定。

Methods: Differences of cellular and extracellular soluble protein expression between high cariogenic potential streptococcus mutans 593 clinical isolate and low cariogenic potential streptococcus mutans 18 clinical isolate were compared by SDS-PAGE.

采用SDS-PAGE技术，比较分离自高龋患者的变形链球菌593号菌株和无龋健康人的变形链球菌18号菌株的菌体和菌外可溶性蛋白表达的差异。

Methods1 EAE was induced by being injected guinea pig spinal cord homogenatein complete Freunds adjuvant in the four footpads and different concentration bordetella pertussis vaccine in one footpad .2 The female wistar rats were randomly divided into control group, EAE groupand Tri group.

材料与方法 1。采用四肢足掌皮内注射豚鼠全脊髓匀浆-完全福氏佐剂，并辅以注射浓度为1×10~(11)个菌体／ml或1×10~(10)个菌体／ml的百日咳疫苗，诱导大鼠建立EAE模型。

The hyphae developed after 7 days on Malt extract agar and the teliospore-like structure was observed .

在麦芽汁液体培养基上培养三天，菌体柱状，一端出芽，细胞大小为（3-4.5）х(7.5-15)μm ，放置一个月后没有菌膜形成但有沉淀。

The extract was further iaolated with LC and purified with preparative HPLC, and uridine, adenosine, and thymidine were obtained.

为了提高菌体中总核苷的提取率，比较了干法和湿法提取对RCEF260菌体中总核苷含量的差异。

The results show that though the strain is growing slower in the nitrogen-free medium than in the nitrogen-containing medium, the content of capsular polysaccharides produced by the strain in the nitrogen-free medium is higher than those in the nitrogen-containing medium, the content of capsular polysaccharides produced by the strain in the nitrogen-free medium is higher than those in the nitrogen-containing medium. The capsular polysaccharides produced by the strain in the culture which contains different mineral powders will reach the highest content on the third or the fourth day in its growing period. The strain's capability of releasing potassium from k-feldspar and biotite in the nitrogen-containing medium is higher than that in the nitrogen-free medium because in the nitrogen-free medium, the strain and its production of glucoprotein are less than those in the nitrogen-containing medium.

结果表明，尽管该菌在无氮培养条件下的菌体数量远小于有氮培养条件，但无氮培养条件下所提取的细菌多糖多于在有氮条件下培养所提取的细菌多糖；胶质芽孢杆菌在以添加钾长石粉或黑云母粉制作的无氮培养基中生长可形成大量多糖，采用丙酮法进行细菌培养液多糖提取，发现细菌培养的第3天所提多糖含量最高；胶质芽孢杆菌在以添加钾长石粉或黑云母粉制作的有氮培养基中生长亦可形成较多的多糖，且在细菌培养的第4天所提多糖含量最高；胶质芽孢杆菌在有氮条件下对含钾矿物的释钾率高于在无氮条件下的释钾率，这可能与该菌在有氮条件下生长更快、可产生较多菌体细胞有关。

Compared to the surface of cellobiose-grown cell, wave-like exocellular protuberances located on the surface of cellulose-degrading cell.

在透明圈中参与纤维素降解的菌体周围具有波纹状突起结构，并且菌体与纤维素的粘附位点是一个电子致密区域。

Methods The influence of inducer concentration and medium on the bacterial density and expression level of target protein in Erlenmeyer flask, as well as the influence of time for induction, method for fed batch and dissolved oxygen on those in 10 L fermentor, were explored.

在三角烧瓶中，探讨不同的诱导剂浓度和培养基对菌体密度和目的蛋白表达量的影响；在10L发酵罐中，探讨诱导时间、补料方式、溶氧量对菌体密度和目的蛋白表达量的影响。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。