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NJWGY3665 bacterial strain is inoculated in a glucose nutrient culture medium to carry out the slant culture, the culture temperature is 28 to 37 DEG C and the culture time is 2 to 6 days;(2) seed culture: a spore which is cultured on a slant is produced into a single-spore suspension by using sterile water, which is also inoculated in a seed culture medium for culture, the temperature is 28 to 32 DEG C, the rotational speed is 200rpm and the culture lasts for 2 to 6 days;(3) fermentation culture: the seed liquid is inoculated in a fermentation culture medium for culture, the temperature is controlled at 28 to 37 DEG C, the pH is controlled at 6.0 to 9.0, the rotational speed is 180 to 220rpm, the fermentation lasts for 5 to 9 days, so as to obtain the Streptomyces sp., methanol or ethanol is used for the extraction and separation of mycelium through the method of centrifugal separation, then the resin method is adopted for carrying out refining, so as to obtain the peptide antibiotics enramycin.

NJWGY3665菌种接到葡萄糖营养培养基中,进行斜面培养,培养温度28-37℃,培养时间2-6天;(2)种子培养:用无菌水将斜面上培养的孢子制成单孢子悬液,并接种到种子培养基中培养,温度为28~32℃,转速为200rpm,培养2-6天;(3)发酵培养:将种子液接种到发酵培养基中培养,温度控制在28-37℃,pH控制在6.0-9.0,转速180-220rpm,发酵5-9天,得到链霉菌,采用离心分离的方法将菌丝体用甲醇或乙醇进行提取分离,再采用树脂方法进行精制,获得多肽类抗生素安来霉素。

Some strains of the nematode-bearing bacteria were bioassayed for their parasitism. All these bacteria can totally not grow or grows badly on the media incorporating the extracts of pine trees, indicating some substances existing in the trees will harmful to the bacterial strains, which is further convinced by preliminary inoculation showing that single bacterial species will not infect Pinus thugbergii. Hence, the growth and reproduction of the nematode-carrying bacteria, which could produce toxic substances causing the death of the tree cells, is closely related to the nematodes, when they first enter to the trees. In the same time, the activity of bacteria improved the circumstances for which nematodes are living, which in turn will beneficial to the growth of nematodes. Thus, there is a complicated interaction among nematodes, bacteria, and pine trees.

对松材线虫携带的细菌的部分菌株进行了寄生性测定,表明这些细菌在添加松树提取液的细菌培养基中不能生长或基本不能生长,这说明松树体内可能存在某些不利于细菌生长的物质,同时初步的接种试验证实,单独的细菌接种不能感染健康的黑松,因此,线虫携带的致病细菌在最初进入树体时,其生长和繁殖与线虫密切相关,结合细菌产生的毒性物质可造成黑松细胞死亡的情况,说明细菌的生长与线虫有关,同时,细菌的活动改变了线虫在松树内的生长环境,使环境变得有利于线虫的生活,因此,线虫、细菌和松树三者存在着复杂的生物关系。

Mycorrhizae are symbiotic associations of fungi and plant roots.

菌根是真菌和植物根的共生体。

Due to insect-borne transmission and systemic infection, paulownia witches'-broom caused by phytoplasma, has created a serious epidemic in Taiwan.

由非培养性植物菌质体引起之泡桐簇叶病(paulownia witches'-broom, PaWB),由於虫媒传播及系统性感染,在台湾普遍严重发生为害。

Armigera. The mRNA expression levels of CYP9A12 and CYP9A17v2 in midgut were higher than those in the fatbody respectively.

以pYES2作为表达载体,在酵母W菌株中表达了棉铃虫细胞色素P450基因CYP9A12和CYP9A17v2,分析了这两个基因的异源表达产物对溴氰菊酯、氟氯氰菊酯、甲氰菊酯和联苯菊酯四种拟除虫菊酯类药剂的离体代谢作用。

And the ribosomal intergenic sequence analysis was used to show the changes of microbial community during the period.

并采用核糖体基因间隔序列分析技术解析了高效菌投加前后污泥系统的群落变化。

Capacity ofcarbon flow through HMS regulated the biosynthesis of chlortetracycline to a great extent.

12%的蔗糖、0.5%甘氨酸、溶菌酶酶解lh,是金色链霉菌原生质体制备的较优条件。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。