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A transposon mutagenesis system applicable for Xanthomonas campestrispv.campestrishas been developed by transferring the transposonTn5gusA5,which contains the gus reporter gene,to the broad host rangvector pLAFR1 and taking the advantage of incompatibility between pLAFR1and pPH1JI plasmids.Five different types of exopolysaccharidedeficient mutants related respectively to eight different mutated sites wereisolated by applying the system to mutagenize the Xcc wild type strain.

本研究将含gus报告基因的转座子Tn5gusA5转座到广谱宿主载体质粒pLAFR1后,利用pLAFR1与pPH1JI质粒的不相容性,建立了一套适合于甘蓝黑腐病菌的转座子诱变体系,应用该体系诱变甘蓝黑腐病菌野生型菌株,成功地分离到5种不同类型、分别涉及8个不同突变位点的胞外多糖突变体。

Methods: Thirty extracted human molars whose pits and fissures on the occlussal surface had been inoculated with Escherichia coli were randomly divided into three groups: group A was treated with newly confected phenol-formaldehyde resin which resinified the dental fissure contents,group B was sealed Bis-GMA resin in pits and fissures and group C as the control group was only treated by cleanin...

选择30颗人离体磨牙,在咬合面点隙裂沟接种大肠杆菌,随机分为3组;A组以新配制的酚醛树脂使点隙裂沟内容物树脂化,B组以成品Bis-GMA封闭剂作点隙裂沟封闭,C组仅进行表面清洗作为对照组;各组分别沿裂沟点隙处片切牙齿,放入LB培养基培养,观察大肠肝菌生长情况。

Methods: Thirty extracted human molars whose pits and fissures on the occlussal surface had been inoculated with Escherichia coli were randomly divided into three groups: group A was treated with newly confected phenol-formaldehyde resin which resinified the dental fissure contents, group B was sealed Bis-GMA resin in pits and fissures and group C as the control group was only treated by cleaning.

选择30颗人离体磨牙,在咬合面点隙裂沟接种大肠杆菌,随机分为3组;A组以新配制的酚醛树脂使点隙裂沟内容物树脂化,B组以成品Bis-GMA封闭剂作点隙裂沟封闭,C组仅进行表面清洗作为对照组;各组分别沿裂沟点隙处片切牙齿,放入LB培养基培养,观察大肠肝菌生长情况。

Nursing Biqing disinfection fluid, is the world's a better health care products, the use of efficient broad-spectrum disinfectant as a major food-grade raw materials, of such as E. coli, Staphylococcus aureus, Candida albicans, mold, fungi and other bacteria propagule to kill a significant role.

碧清消毒护理液,是目前世界上较为理想的卫生护理产品,采用高效广谱食品级消毒剂作为主要原料,对如大肠杆菌、金黄色葡萄球菌、白色念珠菌、霉菌、真菌等细菌繁殖体有显著杀灭作用。

Through controlling and inspecting sanitation, realizing the present pollutive situation of fermentor, the main factor causing contamination of fermenting mash was that the cleaning of fermentor didn't reach the cleanness degree in microbes in the beer production.

通过卫生监控,进一步了解发酵罐的污染状况,发现啤酒生产中,对发酵罐罐体的清洗未达到微生物清洁度,是导致发酵液污染杂菌的主要原因。

For the E. coli lac operon, write the partial diploid genotype for a strain that will produceβ-galactosidase constitutively and permease by induction.

以大肠杆菌的乳糖操纵元为例,请写出一菌株的部分二倍体基因型:β-半乳糖苷酶是组成型表达的,透过酶是诱导表达的。

There are nine fimbiral operons identified in the genome of Klebsiella pneumoniae NTUH-K2044, a highly invasive strain isolated from Taiwan university hospital, by the HMMER search.

利用生物资讯分析方法HMMER,我们在一株从台湾大学附设医院的病患身上所分离出具有高致病性的NTUH-K0244菌株的基因体中找到九套线毛基因组。

Two outstanding fusants SR-7 and SR-T19 were chose out in the fusion group 90-11×FR-008, the former produces not only antibiotics the parents produced but also a new antibiotic effective gainst a wide range of both gram positive and negative bacteria, which the parents could not produce, and its product of the 37℃ fermentation was very effective against the cotton seedling damping off caused by Rhizoctonia sonali while product of the 26℃ was effective agaist the bacterial rots of turnip and Chinese cabbage; the latter have a much stronger activity against a wide range of fungi and yeasts than the parents.

在90-11×FR-008融合组合中,筛选获得2个优良融合子SR-7和SR-T19,前者除产生亲本产生的抗生素外,还产生亲本不产生的一种新的具有广谱抗细菌活性的碱性水溶性抗生素,防病试验表明,其37℃发酵产物对棉花苗期立枯病具有良好的防治效果,28℃发酵产物对萝卜和大白菜具有明显的防腐作用;后者与亲本FR-008的抗菌谱完全一致,但其活性强度都超过了FR-008;采用RAPD分子标记技术检测了SR-7和SR-T19与亲本的同源性及彼此间的差异,从而首次直接从DNA分子水平上验证了链霉菌的原生质体融合子及其遗传重组的多样性。

The result showed that this strain could colonize in many plants, such as capsicum, tomato, eggplant, cabbage, lettuce, cowpea, wheat, corn and rice.

结果表明:该菌株的内生宿主范围较广,可以通过种子、叶片表面以及根际土壤等进入辣椒、番茄、茄子、白菜、生菜、豇豆、小麦、玉米、水稻等多种植物体内定殖。

The stainless steel contains an Ag-Cu binary master alloy with a weight percentage of 0.10 to 4.0 percent and the weight percentage for the rest chemical components are as follows: C less than or equal to 0.08 percent, Si less than or equal to 1.0 percent, Mn less than or equal to 2.0 percent, P less than or equal to 0.04 percent, S less than or equal to 0.03 percent, Ni 7.0 to 15.0 percent, Cr 17.0 to 20.0 percent, Mo less than or equal to 4.0 percent, N less than or equal to 0.15 percent and the rest are Fe and unavoidable impurities.

本发明公开了一种奥氏体抗菌不锈钢及其制造方法,所述不锈钢含有重量百分比为0.10~4.0%的Ag-Cu二元中间合金,其余化学成分重量百分比为:C:≤0.08,Si:≤1.0,Mn:≤2.0,P:≤0.04,S:≤0.03,Ni:7.0~15.0,Cr:17.0~20.0,Mo:≤4.0,N:≤0.15,其余为Fe和不可避免的杂质。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。