菌体

Colorants biodegradation and growing condition of the Polystictus versicolor cultured with simulation effluent were investigated.

本文研究了云芝菌株在模拟废水中生长，获得对废水较高的漂白效果以及最大菌丝体产量的培养基成份为：模拟废水培养基中初始外加碳源-葡萄糖添加量为1%、氮浓度范围是6～12mmol/L、CaCl2浓度为0.05mol/L、RS为消泡剂。

When attacked by this isolate, the color of young Porphyra gametophytes turned green.

当遭到此菌侵害时，幼苗期的紫菜配子体的颜色变绿。

For L. barbarum transformation, the optimized procedure was using 3-week-old leaf explants, preculture for 3-4 days, infection with 1∶1 dilution of an overnight culture of EHA101 (pIG121Hm) suspension with AS and co-culture for 3 days at 24℃ in the dark.

通过农杆菌转化条件的优化，将AS活化的过夜培养EHA101（pIG121Hm）菌液稀释一倍后感染预培养3—4天的三周龄叶外植体，并在24℃黑暗条件下共培养3天，可以获得较为理想的转化效率（62.5％）。

After having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time,incubation time and co-culture time,we set up the system of high frequency transformation of tomato cotyledons.Then hBMP-3m gene was transferred into tomato via Agrobacterium-mQdiated transformation,and the resistant plants to Hyg were obtained.

建立了以番茄子叶为外植体的遗传转化体系，确定了预培养时间、浸菌时间及共培养时间等转化条件，建立了番茄高频转化系统，然后通过农杆菌介导法将hBMP-3m基因导入番茄，并且获得了Hyg抗性植株。

Two organisms(SG1,SG2)were isolated from the affected birds,which were identified as Chlamydia psittaci by means of electromicroscopic observation,artificial culture media inoculation test,sulfanilamide sensitivity test and iodine staining.

从发病鸽分离到 2株菌(SG1、SG2 )，经电镜形态学观察、人工培养基接种试验、碘胺敏感性试验及碘染色试验鉴定为鹦鹉热衣原体。

Abstract］ Objective: To provide a suitable fungal material for protoplast-mediated genetic transformation and mutation induction of Pythium guiyangense Su.

目的：建立贵阳腐霉原生质体的制备和再生系统，为该菌的基因工程和细胞工程改良提供适宜的真菌状态。

Adopt optimized transformation system of the melon cultivar: the explant infected in the engineering bacterium the OD560 of which was 0.30 for 15min, and co-cultured for 3days; Choosing cultivated in MS +1mg/L 6 - BA + 500mg/L carb + 75mg/L Kan, extend cultivated in MS +0.05mg/L 6 - BA + 300mg/L carb + 75mg/L Kan and rhizogenic cultivated in 1/2MS + 200mg/L cef + 25mg/L Kan.

对甜瓜栽培品种的转化采用已优化好的转化体系：农杆菌菌液OD560=0.3，侵染时间为15min，外植体选用近轴端子叶；共培养时间为72h。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，將溶酶体的靶向信號肽KFERQ连接在RTA的羧基端；將构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受態大肠桿菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

In order to reach a higher productivity level of mycelium and exopolysaccharide ,L934 orthogonal experiments on fermentation with Schizophyllum commune were carried out in 7 L fermentor.

在7 L发酵罐上进行了裂褶菌发酵生产菌丝体和胞外多糖的研究，采用L_9(3~4)正交试验对发酵条件进行了优化。

Schizophyllum commune is a kind of edible and medicinal fungi,which contains different kinds of bio—active constituents,such as polysaccharides.

在7 L发酵罐上进行了裂褶菌发酵生产菌丝体和胞外多糖的研究，采用L_9(3~4)正交试验对发酵条件进行了优化。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。