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The excellent characters of the hyperparasitic Streptomyces F46 and the Streptomyces SC1 were tried to integrate into a fusant by protoplast fusion. The study mostly included content as follows: First, the parental strains were identified by two approaches of the classic identify and the molecular taxonomy.

本试验利用原生质体融合技术将重寄生链霉菌F46和链霉菌SC1融合,以期得到整合双亲优良特性于一体的生防菌。

The multi-drug resistance rate of Salmonella and E.coli were 91.80% and 100%.According to the genes sequence published in Genbank ,we analysed the value of Tm ,content of G+C, oligonucleotide dimer by DNAStar and Primer 5.0 software, and according to the construction of I integron ,we designed 3 pairs of primers to amplify intIl gene ,variable segment ,3"-conserved segment for detectting Salmonella and E.coli"s I integron.As a result , aimed genes were amplified and sequenced;The detection rate of I integron of 61 Salmonella and 48 E.coli were 81.97% and 66.67%.

据文献报道及GenBank中注册的基因序列,运用DNAStar软件分析同源率及Primer5.0软件对Tm值、G+C含量、引物二聚体等进行分析,根据Ⅰ类整合子的结构设计了3对引物,对Ⅰ类整合子的intI1、可变区、3'保守端进行扩增,检测沙门氏菌和大肠杆菌Ⅰ类整合子;扩增出了相应基因片段并测序;61株沙门氏菌和48株大肠杆菌中分别有50株和32株菌检测出Ⅰ类整合子,Ⅰ类整合子的检测率分别为:81.97%和66.67%。

The positive rates of hemorrhage in rat lungs and leptospira in rat kidney were 1.12% and 0.31%.

鼠肺出血热带毒率为1.12%,鼠肾钩体带菌率为0.31%。

Methods Collected the eggs of Lucilia sericata, artificial rear the maggot under the experimental condition, Observed the time orderliness of maggot living.

方法搜集丝光绿蝇虫卵,在实验室无菌条件下进行人工饲养与繁殖,观察蛆虫生长时间与规律,对虫卵和虫体采用双重消毒处理,对蛆虫进行细菌学和病毒学检测。

The bioactivities of phenolic and essential oil extracted from clove leaves in vivo and vitro against Penicillium expansum, Botrytis cinerea, Colletotrichum gloeosporioides, Macrophoma kawatsuka, Monilia fructigena and Trichothecium roseum, and their effects on the quality of postharvest apple fruits were studied.

该文对丁香精油和丁香酚在离体和活体条件下对采后苹果的主要致腐菌苹果青霉、苹果灰霉、苹果炭疽、苹果轮纹、苹果褐腐和苹果腐心的抑制作用以及对贮藏期苹果品质的影响进行了研究。

Cell line transformed with A〓 in Medicago sativa could not regenerate.

利用发根农杆菌A〓和LBA9402Bin19两个菌株对苜蓿和红豆草不同外植体进行了转化。

Morchella esculenta ; liquid fermentation ; mycelial biomass ; extracellular polysaccharide

羊肚菌;深层发酵;菌丝体生物量;胞外多糖

According to morphous characteristics,it was regarded as Phanerochaete chrysosporium.

由正交试验确定出该菌原生质体制备和再生的最佳条件:酶浓度为1.5mg/mL,酶解时间为2h,酶解温度为30℃。

Objective: To study the incidence of mycoplasmagenitalium infection in non-gonococcal urethritis/mucopurulent cervicitis patients.

目的探讨生殖支原体在非淋菌性尿道炎/粘液脓性宫颈炎中的发病情况。

This result fitted with the selection standards of the optimum mutagenic dose of the microbe chemical mutagenesis.Different...

不同毒性的小麦条锈菌生理小种对EMS的敏感性不同,且在离体培养条件下,毒性较弱小种的生活能力及抗逆能力明显强于毒性较强的小种。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

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关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。