菌体

As a result, Core gene was expressed in E.coli with yields of 20% of total proteins and it was a 31KD fusion protein on SDS-PAGE and Western-Blot, it shows that Core protein was highly expressed in E.coli;One fragment of 369bp could be seen on the gel of the Core gene PCR product. A band of 29KD could be seen on the Western-blot after induction of yeast containing pPICZaA-Core for 72 hours. This indicate that Core protein was secretively expressed in the Pichia pastoris with weak yields.

结果显示pBVIL1-Core的表达产物经SDS-PAGE分析出现一条约31KD的带，与预期融合蛋白的分子量相符，表达蛋白存在于包涵体中且表达量占菌体总蛋白的20%，Western-blot显示诱导后菌体在相应位置出现特异性杂交带，表明

Significant loss of GEMs was not observed but strong growth activities, formation of biofilm and zoogloea on the surface of immobile particles were observed via scanning electron microscope. The mechanical stability of the particles remained unchanged after nearly two-month operation.

在整个反应过程中，没有出现大量工程菌流失的现象，同时在固定化颗粒的表面以及浅层均观察到了大量工程菌菌体，固定化颗粒的表面还出现了生物膜和菌胶团，反应结束时，颗粒形态完好，强度满足本工艺条件下长期使用的需求。

C. I. Reactive blue KN-R, Alizarin brilliant green G and Hostlam blue R could not be degraded by Zoogloea HP3. Furthermore, ABAS degradation was regressed under the exisitence of the above dyes. 1-Aminoanthraquinone-2sulfonic sodium, 1, 4-dihydroxylanthraquinone-2-sulfonic sodium, 1, 4, 5, 8tetrahydroxylanthraquinone, anthraquinone, aniline, phenol, catechol and ophthalic acid could be degraded by Zoogloea HP3. Aniline was the most degradable substrate among ABAS, aniline, phenol, catechol and o-phthalic acid. However, benzenesulfonic sodium and p-amino benzenesulfonic sodium were not degraded by it.

动胶菌HP3不能降解活性艳蓝KN-R、弱酸性绿GS及毛用活性蓝HW-R等蒽醌型染料，且染料的存在对菌体降解溴胺酸有不同程度的抑制作用；动胶菌HP3可以降解1-氨基蒽醌-2-磺酸钠、1，4-二羟基蒽醌-2-磺酸钠、1，4，5，8-四羟基蒽醌、蒽醌及苯酚、邻苯二酚、苯胺、邻苯二甲酸等苯系化合物，而不降解苯磺酸钠、对氨基苯磺酸钠；在溴胺酸、苯酚、邻苯二酚、苯胺、邻苯二甲酸中，苯胺是动胶菌HP3的天然底物。

The uranine staining can be used to observe the whole growing phases of Sphaerotheca fuliginea, while aniline blue staining is suitable for conidial observation.

荧光素钠可以观察到菌体整个发育阶段。苯胺蓝只适合于前期菌体入侵过程的观察。？

The antibacterial activities of most of fractions were similar to the pomegranate peel extract.(7) Respiratory inhibition experiment showed that the respiration of thallus was inhibited mainly through the hexose monophosphate pathway, which leaded to be short of NADPH, and induced metabolic function dysfunction. Growth curve assay showed that the pomegranate peel extract inhibited cytodieresis in logarithmic growth phase. After exposure to the pomegranate peel extract, bacterial protein was analyzed by SDS-PAGE. The results showed that protein synthesis was inhibited, especially for large molecular weight protein.

7呼吸抑制试验结果表明，石榴果皮提取物主要是通过抑制HMP途径，导致还原力NADPH缺乏，引起代谢功能障碍，达到抑菌的目的；加药组和正常组金黄色葡萄球菌生长曲线的对比结果表明，石榴果皮提取物能够抑制金黄色葡萄球菌对数生长期的菌体分裂；SDS-聚丙烯酰胺凝胶电泳分析表明，石榴果皮提取物能够抑制菌细胞蛋白合成，尤其对菌体内大分子量蛋白作用最明显。

Within the range of 200mg/L—1000mg/L, the more the initial concentrations of ABA, the more the decolorize velocity of it. The functional relationships between specific growth rate of N1 and temperature, degradation rate of ABA and temperature were constructed, respectively.

通过测定菌株的生长曲线和降解曲线，表明N1菌株的菌体生长和溴氨酸的降解过程是同步进行的；N1菌株对溴氨酸的脱色作用是酶促降解反应的结果，脱色酶需经溴氨酸诱导产生；在一定浓度范围内（200mg/L—1000mg/L），随着溴氨酸浓度的增加，其脱色速度逐渐变慢。

The results from D6's morphological characters, colonial type, Gram's staining and a series of physiologic and biochemical experiments reveales that the strain of D6 is Desulfovibrio vulgaris Postgate and Campbell.

根据菌体形态、菌落特征、革兰氏染色和一系列生理生化试验结果，证明：D6菌属于脱硫弧菌属的种，名为普通脱硫弧菌Desulfovibrio vulgaris Postgate and Campbell。

Results of cultural characters show that Grifola frondosa has different macroscopic arid microscopic culture characteristics on different media, and the mycelia on PDA medium and half-combined PDA medium can produce extracellular phenol oxidase, indicating that G. frondosa is a kind of white-rot fungus. On PDA medium and half-combined PDA medium, hyphae with thick colonies, dense, white, villiform to flocky, grow very well, but aerial hyphae grow faintly and loosely on MEA medium.

培养特性的研究结果表明，灰树花在不同的培养基上宏观和微观培养特性各有不同：菌体在PDA和马铃薯半组合培养基上都能够产生细胞外酚氧化酶，说明灰树花是一种白腐菌；菌丝在PDA和马铃薯半组合培养基上生长旺盛，浓密，白色，绒毛状至絮状，菌落厚；在MEA培养基上生长较弱，气生菌丝体较稀疏。

After transfection of E.coli BL21 and induction by IPTG,the recombinant immunotoxin fusion protein was highly expressed in E.coli BL-21 in the form of inclusion bodies(up to 32.29% of total cellular proteins).

IPTG诱导后目的蛋白获得高效表达，SDS-PAGE分析目的蛋白主要以不溶性包涵体的形式存在于菌体中，表达量占菌体总蛋白的32.29％。

Feeding of glucose was started at the end of the exponential phase of yeast growth. Intermitted aeration for 10 sec at every 3 min interval was used for the fed-batch fermentation. A dry cell weight of 8.22 g/L and an ethanol concentration of 73.7 g/L were achieved after 60 hrs of cultivation.

以葡萄糖浓度275 g/L进行批次馈料酦酵培养，当菌体生长至对数生长的末期时，通气方式改变为每间隔3分钟通气10秒钟，於60小时后菌体乾重可达到8.22 g/L，酒精浓度为

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。