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On the media containing 0.02 and 0.04 g/L potassium sorbate, the contamination rates of explants were 87% and 97% resp. The bacteriostatic effect of 0.02 g/L potassium sorbate was obviously better than that of its other concn.

在含0.02和0.04 g/L山梨酸钾的培养基上外植体的污染率分别为87%和97%。0.02 g/L山梨酸钾的抑菌效果明显好于其他浓度。

Bioassay in vitro revealed that the EC50 values of compounds 1 and 2 against sporangium germination of P. litchii were 100.28 mgmL^(-1) and 124.37mgmL^(-1), respectively.

离体抗菌活性测定化合物1和化合物2抑制荔枝霜疫霉菌孢子囊萌发的EC50分别为100.28mgmL^(-1)和124.37mgmL^(-1)。

Variations in hyphae diameter, sporulation and vesicle numbers were noted between media and also between isolates.

菌株在菌丝体大小、孢囊形成数量、泡囊形成等方面存在着明显的差异,并随着培养基成分而变化。

Cell elongation in the middle and upper middle stipe regions accounted for most of the stipe elongation but this was accompainied by cell divisions.

在原基体时期其快速生长区间菌柄细胞数为其他部位的四倍,其细胞生长主要在基部最为活跃,而非其边缘细胞。

Transferred the NP-1 gene into C. ellipsoidea by electroporation with high efficiency expression vector pBinUΩNP1. PCR, Southern blot and Northern dot blot analysis of the G418resistant alga strains indicated the stable integration and correct transcription of NP-1 in C. ellipsoidea genome. In vitro antagonistic assays showed the proper expression of defense gene in C. ellipsoidea. Transgenic C. ellipsoidea is antagonistic to gram negative bacteria—Bacillus subtonic, gram positive bacteria—Escherichiacoli and fungi—Fusarium oxysporum to some extent.

以椭圆小球藻完整细胞为受体,采用电激转化法将连接在高效表达载体pBinUΩNP1上的兔防御素NP-1基因导入小球藻,通过PCR、Southern杂交、Northern点杂交对转基因小球藻进行了分子检测,同时通过体外离体抑菌实验检测了兔防御素NP-1基因表达产物的活性,结果表明NP-1基因已稳定整合到小球藻基因组中,并进行了正确转录和表达。

Drugs (methimazole, methylthiouracil, propylthiouracil and thiouracil); some sedatives (doxylamine succinate and phenobarbital); and some other drugs including the systemic antifungal antibiotic griseofulvin, the diuretic spironolactone, and the antibacterial sulfa drugs sulfamethazine and sulfamethoxazole.

抗甲状腺?药物(他巴唑,甲基硫氧嘧啶,丙基硫氧和硫氧嘧啶);一些镇静剂(doxylamine琥珀和苯巴比妥),以及包括系统性真菌抗生素灰黄霉素,安体舒通的利尿一些其他药物,以及抗菌磺胺类药物和磺胺二甲嘧啶。

Our experiment is based on Chlamydia trachomatis cell culture to detect the in vitro susceptibilities of C.

为此,我们通过沙眼衣原体细胞培养,从天津市性病研究所就诊的病人泌尿生殖道分离出30株C.t野生菌株,并运用碘液染色计数包涵体的方法检测了这些野生株在体外对3种常用的抗菌药物四环素、阿奇霉素、盐酸莫西沙星的敏感性。

Progress in the study of the influences of light, water, herbivory and fungal symbiont on the growth and survival of Dipterocarpaceae seedlings is briefly summarized, and the prospects in the future are also discussed.

就光照、水分、动物捕食和菌根共生体对龙脑香科植物幼苗生长与死亡的影响进行了综述,并对未来研究的发展方向作一展望。

The main results were as follows:①The genomic DNA extraction method from TCK/TCT teliospore was established, which include cell wall lysis of teliospore by alkali solution, destroying teliospore cell wall for releasing nucleic acid from cytoplasma membrain and elimination of the PCR inhibitors such as pigments or polyphenols component.

论文主要研究结果如下:①优化了碱裂解法破冬孢子外壁-CTAB破坏原生质体,释放核酸-微量DNA过滤柱去除色素、多酚等PCR抑制物质的TCK/TCT冬孢子提取方法,排除了冬孢子细胞壁难破DNA难以提取,三甲胺、色素等PCR抑制物质导致假阴性的难题,采用此方法提取的腥黑穗菌DNA完全适用于PCR扩增。

ConclusionTest tube tuberous root of Pueraria thomsonii Benth.could be induced in sterile conditions in vitro.

结论无菌离体条件下可以诱导出粉葛试管块根。

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