菌体

The specific PCR products of approximately 112bp in length was amplified from the other 10 strains of E.faecalis from lamb naturally infected with E.faecalis,lamb and mouse experimentally infected with E.faecalis by the PCR assay,but no any bands were amplified from Streptococcus equi subsp.equi,Streptococcus G,Streptococcus A,Staphylococcus aureus,Listeria monocytogenes from sheep,Mycoplasma ovipneumoniae and Brucella ovis.

应用该方法对分离的致羔羊脑炎粪肠球菌、由肠球菌导致发病或死亡羔羊脏器以及人工感染死亡小鼠的主要脏器进行扩增，均能得到特异性的DNA条带；对其他7种病原菌，马腺疫链球菌C群、G群链球菌、A群链球菌、金黄色葡萄球菌、羊源李氏杆菌、绵羊布氏杆菌、绵羊肺炎支原体不能扩增出特异的DNA条带。

The isolated strains were subjected to phenotypical study,salt-aggregation test and rDNA ITS sequencing.

对获取的活性菌株进行形态特征研究、盐需求测试、核糖体rDNA ITS区序列分析，并将所测得序列在NCBI数据库中进行相似性搜索，选取其中相似性较高的序列以邻接法构建系统进化树，将该菌株分类鉴定到种。

Transformed roots were inocuiated with resting spores of Plasmodiophora brassicae. On solid MS medium, the resting spores infected root haires but not root cortical tissues, and therefore no syndrome of club robt disease was found.

用根肿菌休眠孢子接种离体培养的转化根，结果发现，在固体培养基上，根肿菌只侵入根毛内，不侵入根皮层组织，因而不表现根肿病的任何症状。

Methods Acanthamoeba polyphaga and Legionella pneumophila were co-cultured under laboratory condition. At consecutive time points during the culture, smears of the cultured products were made on glass slides for staining purposes. Different types of stainings including Gram′s staining, Gimenez staining, Giemsa staining and immunofluorescence were used to determine the best method for the identification of amoebal pathogens.

方法嗜肺军团菌与多噬棘阿米巴共培养，取不同时点的共培养物制作涂片，采用革兰氏染色、吉曼尼兹染色、姬姆萨染色、免疫荧光染色等多种方法，用光学显微镜及荧光显微镜观察鉴别阿米巴滋养体与其胞内嗜肺军团菌，并比较这些染色方法的效果。

This paper studied the conditions of protoplast preparation of Oenococcus oeni,including the concentration of preculture and lysozyme,lytic time,hypha culture time,etc.

通过对预处理剂质量浓度、酶质量浓度、酶解时间、菌龄等影响因素的分析，以及渗透压稳定剂的筛选，对酒酒球菌SD-2a原生质体的制备条件进行了研究。

This paper studied the conditions of protoplast preparation of Oenococcus oeni,including the concentration of preculture and lysozyme,lytic time,hypha culture time,etc.As a result,hypha culture time 20 h,penicillinG Na 0.5 μg/mL,lysozyme 1 mg/mL,lytic time 30 min,SMM as osmotic pressure stabilizer,ATB culture medium of regeneration culture mediu...

结果表明，酒酒球菌SD-2a原生质体制备的最佳条件为：菌龄20 h，青霉素G钠质量浓度0.5μg/mL，酶质量浓度1mg/mL，酶解时间30 min，渗透压稳定剂采用SMM缓冲液，再生培养基采用添加0.7 mol/LKCl的ATB培养基，再生培养时采用双层培养基厌氧培养，可以保证较好的形成率和再生率。

The DNA of Yak PrP expressed plasmid extracted from the recombinant expressed bacterium was identified by PCR amplification and two-ezyme digestion. The expressed product was obtained from the recombinant bacterium inducted under the optimized expressed condition (37℃, 1 mmol/L IPTG, 6 h), and it was determined by SDS-PAGE and Western blotting. The GST-BoPrP(23—242) fusion proteins were collected by two ways: the first is to purify and renature from the inclusion bodies; the second is to separate by SDS-PAGE.

将保存的含有牦牛PrP基因重组表达菌提取质粒DNA，进行PCR扩增和双酶切鉴定，并在优化诱导表达条件(37℃，1mmol/L IPTG，6h)下，获得的表达产物进行SDS-PAGE和Western blotting检测；将鉴定之后的重组表达菌诱导表达后，通过两种方法回收GST-BoPrP(23～242)融合蛋白：其一，是从包涵体中提取和复性GST-BoPrP(23～242)融合蛋白；其二，是通过SDS-PAGE直接分离并纯化GST-BoPrP(23～242)融合蛋白。

The impacts of urediniospore activity, concentration of hydrochloric acid and physiological races of rust fungi on protoplast shrinking were also studied.

研究还发现叶锈菌和秆锈菌夏孢子活力、盐酸浓度等对夏孢子原生质体浓缩状况有很大影响，且与病菌生理小种有一定的关系。

By means of the effect of cooperation of microorganisms, the Elsholtizia spledens plant was composted quickly.

通过纤维素分解菌和EM菌的协同作用，能够快速高效地降解海州香薷残体。

Mycorrhiza is an international journal devoted to research into mycorrhizas - the widest symbioses in nature, involving plants and a range of soil fungi world-wide.

菌根》杂志是国际性刊物，致力于菌根类生物（自然界中最广泛的共生体）研究，包括植物和土壤真菌。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。