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In vitro, the inhibitory activity of the fungicide against mycelial growth of both sensitive and resistant isolates of Fusarium graminearum was measured and the influence of the fungicide on conidial germination of Fusarium graminearum was determined.

离体条件下,采用菌丝生长速率法测定了该药剂对禾谷镰孢菌抗性菌株和敏感菌株的生长抑制活性;同时采用孢子萌发法测定了其对禾谷镰孢菌分生孢子萌发的影响。

The antifungal activities of these materials were investigated and build foundation to develop low poison plant antifungal products. In experiment, polyphenols were extracted by reflux ectraction method which applyed petroleum ether to degrease and methyl alcohol solvent. Phenolic hydroxyl were examined and inspected by colour-developing reaction and the content of total polyphenols was determined by UV-spectrophotometry. Screening test were performed to the antifungal activities of polyphenol extraction by the tube dilution method to the selected ten pathogenic fungus such as Dothiorella gregaria, Rhizoctonia solani, Phyllosticta pirina, and the minimal inhibitory concentration were measured. The column chromatography and thin layer chromatography were applyed to separate and analyse cortex extracts of Dracaena cochinchinensi Lour.

实验部分采用以石油醚脱脂,甲醇为溶剂的回流提取法来提取植物多酚,用显色反应检识酚羟基;采用紫外分光光度法对多酚粗体物进行总多酚含量的测定;选取了聚生小穴壳菌、立枯丝核菌、梨叶点霉等10株常见农林业致病真菌,采用试管液基稀释法对多酚粗提取物进行了抗真菌活性筛选试验,并测定其最低抑菌浓度;采用柱层析法和薄层层析法对其中抑菌效果较好的龙血竭进行分离分析,根据其中3种化合物的光谱数据UV、IR、~(13C-NMR、1H-NMR和MS鉴定其结构。

In addition, AM fungi from a wide range of environmental conditions (originally isolated from north, central and south China) were used to study the ecological adaptability of AM fungi and the influence of edaphic conditions on AM fungal growth and metabolic activity.

丛枝菌根共生体由宿主植物和真菌共同组成,菌根真菌的生长、代谢活性、菌丝分布以及生态适应性等特征不但与菌根效应、菌根真菌的生态功能密切相关,而且受到宿主植物和土壤条件的影响。

In macrophages, Legionella destructed the transmission of small bubble, prevented phagosome-lysosome fusion, prevented the meaning of the sterilization of endosome and lysosome, established its own unique copy of the body and swallowed up full of host cells, finally leaded to the host cell death.

在巨噬细胞内,军团菌破坏小泡传输,产生空泡,阻止吞噬体-溶酶体融合,阻止内涵体和溶酶体的杀菌作用,建立其独占的复制型吞噬体,以二***复制,产生大量的吞噬体,布满宿主细胞,导致宿主细胞死亡。

Studies on the mutant breeding of high-yield gibberellins strain The protoplast of Gibberella fujikuroi m978 were treated by mutation breeding forhigh-yield gibberellins, LiCl mutation was better than DES mutation or UV irradiation ifsingle mutation, but any single mutation was worse than compound mutation by whichthe mutants were more overproducting and genetically stable.

采用传统诱变育种,筛选高产菌株在原生质体条件下,对赤霉素产生菌m978的原生质体进行菌种选育;使用单因子诱变方法,LiCl处理的效果优于DES诱变和UV诱变;复合诱变比单因子诱变更容易得到高产突变株,且获得的高产突变株稳定性更优。

The genus specificity was validated with 47 strains including wild type isolates. Preliminary screening of soil samples with Gordonia-specific primers was successful in the rapid detection of the presence of 36 Gordonia wild-type isolates.

经多序列分析比对软体计算并比较gyrB基因序列,可知Gordonia菌属与7种邻近放线菌的gyrB基因序列相似度介於72.8-82.1%,而在16S rDNA序列相似度则介於90.0-95.4%,gyrB基因可明显区分Gordonia菌属与其他属之放线菌。

A diverse range of effects:on protein solubility and secretion was observed,and these effects were highly dependent on the particular chaperone.In the host strain over-expressing chaperone SecB harboroing pSecB,the amount of total periplasmic proteins was increased by about 71%and the activities of alkaline phosphatseand GL-7-ACA acylase(GL7A)were increased by about 54%and l.5-fold respectively.In the host strain over-expressing chaperone GroEL bearing pGroEL,the amount of total periplasmic proteins was increased by 52%,and the activity of penicillin G acylasewas in creased by about 76%;about 90%of Hexameric Calcitonin(Cal6)inclusion body and l5%of MS2interleukin3(MS2-HIL3)inclusion body became soluble respectively.All the data were compared with the parental strains un-bearing plasmdis pSecB or pGroEL.

在过量表达SecB的宿主菌中,周质空间分泌蛋白总量较对照组提高了约71%,GL-7-ACA酰化酶在周质空间酶的活力较对照组提高了约1.5倍,碱性磷酸酯酶在周质空间酶的活力较对照组提高了约54%;在过量表达GroEL的宿主菌中,周质分泌蛋白总量较对照组提高了约52%,青霉素G酰化在周质空间酶的活力较对照组提高了约76%,鲑鱼降钙素六聚体的可落性组分的比例由原来的45%增加到约90%,而MS2-人白介素-3融合蛋白的包涵体有约15%转变为可溶性组份。

The effect of nutritional and enviromental conditions on polysaccharide production by morchella escutenta in liquid fermentation were determined.

以羊肚菌为材料,研究了发酵过程中碳源、氮源、无机盐、培养条件对菌丝体生物量、胞外多糖的影响,以及发酵过程中菌丝体生物量、胞外多糖、总糖及还原糖质量浓度、培养基pH值的动态变化,并在此基础上确定了羊肚菌液体深层发酵的最佳条件。

Under the condition of the plate culture ,the effects of Cd,Pb and their combined pollution on the growth of hyphae of Morchella esculenta were studied on potato dextrose agar culture media,and the bioaccumulation method of hyphae on Cd and Pb in the solid-state media was set up.

在平板培养条件下,研究了镉、铅及其复合污染对羊肚菌菌丝体生长的影响,并建立了羊肚菌菌丝体对生长基质中镉、铅生物富集的方法。

The studies of mycorrhizal structures were helpful to understand the function mechanism of mycorrhiza. It could provide a theoretical base for further application of mycorrhiza.

对菌根共生体结构的研究有助于了解其与根功能的适应及深入探讨菌根的作用机理,为菌根菌的进一步应用提供理论基础。

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