菌体

Based on Phylogenetic analysis of sequences from these 10 phytoplasmas using PAUP (phylogenetic analysis using parsimony) software, these phytoplasmas could be classified into five groups. These phytoplasmas groups and strains were: group 1, sweetpotato witches' broom, mainland China strain of sweetpotato witches' broom, peanut witches' broom and Ipomoea obscura witches' broom; group 2, loofah witches' broom; group 3, elm yellows; group 4, rice yellow dwarf and sugarcane white leaf disease; group 5, New Jersey strain of aster yellows and western strain of aster yellows.

从spacer序列堆衍的亲缘演化树显示这10种植物菌质体可分为5群：第一群，甘薯簇叶病、甘薯簇叶病、花生簇叶病及细花牵牛簇叶病等菌质体；第二群，丝瓜簇叶病菌质体；第三群，榆树黄化病菌质体；第四群，水稻黄萎病及甘蔗白叶病菌质体；第五群，翠菊黄化病菌质体。

Heat treatment on the preparation,structure,electrovalence of silver,releasing speed of Ag ions and antibacterial property of obtained blocks were investigated.A ultraviolet-visible spectrum of Ag-carrying material treated at 600℃ indicates that there are plenty of Ag ions in Ag-carrying SiO 2 .The Ag ions can be released stably into water of 30℃ for 14 d.

考察热处理制度对抗菌块体的制备及结构、银价态、银离子释放速度、抗菌性能的影响。600℃热处理的抗菌块体紫外-可见吸收光谱表明：SiO 2 载体中富含银粒子，并且此抗菌块体在水中能保持较长期（14d）的稳定溶出。

The polypores treated in the present list are in wide sense, including the poroid species in Polyporales, Hymenochaetales, Gloeophyllales, Trechisporales, Corticiales, Thelephorales and Russulales, and a few poroid genera of Agaricales, Atheliales, Cantharellales and Auriculariales (e.g. Dictyopanus, Favolaschia, Elmerina, Fistulina and Protomerulius).

本文中的多孔菌系指广义非褶菌目中具有孔状子实层体的种类，按照现代分类系统包括担子菌门中多孔菌目、锈革孔菌目、褐褶菌目、糙孢孔目、革菌目、糙孢革菌目和红菇目中孔状子实体的种类，还有伞菌目、阿太菌目、鸡油菌目和木耳目中个别属，如网孔菌属、胶孔菌属、榆孔菌属、牛排菌属和纵隔孔菌属等的种类。

Auricularia auricula is of the single-factor controlled mating system according to mating test among the F1 progenies and their parents, combining the method of dyeing the nuclei of the mycelia and observing the nuclear phase under the fluorescence microscope and the method of observing the clamp connection under the light microscope to determine whether the dikaryotic mycelia were formed.

摘 要：从黑木耳种内杂交子H2J3的子实体上单孢分离培养得到F1代52个单核体菌株，将F1子代及亲本(H2，J3)等所有供试单核体菌株的菌丝体两两配对培养，采用核荧光染色观察核相，和光学显微镜观察锁状联合，鉴别菌丝交配反应是否形成双核体菌丝，同时测定了亲本菌株所有F1子代的交配型。

Auricularia auricula is of the single-factor controlled mating system according to mating test among the F1 progenies and their parents, combining the method of dyeing the nuclei of the mycelia and observing the nuclear phase under the fluorescence microscope and the method of observing the clamp connection under the light microscope to determine whether the dikaryotic mycelia were formed.

从黑木耳种内杂交子H2J3的子实体上单孢分离培养得到F1代52个单核体菌株，将F1子代及亲本(H2，J3)等所有供试单核体菌株的菌丝体两两配对培养，采用核荧光染色观察核相，和光学显微镜观察锁状联合，鉴别菌丝交配反应是否形成双核体菌丝，同时测定了亲本菌株所有F1子代的交配型。

In order to improve the ability of degrading anthracene and the survival of exogenous bacteria An, protoplast electrofusion was performed between An which can degrade anthracene effectively and Tu which has high survival ability, and then a fusant with specific properties was selected from the regenerated colonies.

为了提高外源蒽降解菌An降解蒽的能力和存活能力，采用原生质体电融合技术，在蒽降解菌An和土生优势菌Tu之间实行了原生质体电融合，并从再生的融合子F中筛选到具有特定性状的后代菌株。

Epiphytic bacteria were isolated from sporophytic thallus collected from Qingdao and Dalian or gametophytic filaments stored in our lab of Laminaria japonica. Identification was done using both traditional physiological and biochemical way and molecular method. Phylogeny tree was established based on 16S rRNA gene sequence analyses, and some sequences of epiphytic strains from both sporophyte and gametophyte were different from all the sequences already exit and could be the new species.

采用生理生化和16S rRNA基因序列比对的方法对从两个海区采集的海带孢子体以及实验室中保存的海带配子体样品的外生菌分别进行组成分析，16S rRNA基因序列揭示了在海带孢子体和配子体的外生菌中都存在一些与现有数据库中序列的遗传关系较远的菌株。

Methods From Feb 2006 to Jun 2006,188 hospitalized children in Shenzhen children s hospital, were collected deep tracheal aspirate at the time of hospitalization. The respiratory tract secretions were immediately sent for bacterial culture with 3 kinds of medium:ordinary medium, Hemophilus influenzae selective medium, Streptococcus penumoniae selective medium. Then we extracted the total nucleic acids from secretions, and detected Mycoplasma pneumoniae by single fluorescent quantitation PCR. Simultaneously, 14 respiratory tract pathogenic bacterium and Mycoplasma pneumoniae were detected by Target Enriched multiplex PCR. Amplification products were identified by the Luminex100 suspension array.

确诊为社区获得性肺炎的患儿188例，在入院当天采集深部呼吸道吸引物，用普通培养基和肺炎链球菌、流感嗜血杆菌选择性培养基进行细菌培养，然后提取深部呼吸道吸引物中病原体的DNA，采用荧光定量单PCR的方法检测肺炎支原体，并对同一标本采用靶序列富集多重PCR技术同时扩增肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、肺炎克雷伯菌、大肠杆菌、嗜肺军团菌、铜绿假单胞菌、鲍曼氏不动杆菌、脑膜炎奈瑟氏菌、阴沟肠杆菌、奇异变形杆菌、化脓链球菌、粪肠球菌及屎肠球菌14种呼吸道病原菌和肺炎支原体的靶基因，扩增产物用Luminex100多功能悬浮点阵仪检测。

In filicophytes, there was a relatively high ratio of VA mycotrophy in the eusporangiates, and there was no autotrophic in the eusporangiates; but the ratio of autotrophic was up to 71%, and the ratioes of VA mycotrophic and facultative mycotrophic were low, 15% and 14% respectively in the leptosporangiates.

用碱解离、酸性品红染色法对云南热带、亚热带自然生长和部分移栽到温室中的256种（拟蕨类植物20种，真蕨类植物236种）蕨类植物孢子体的VA菌根状况进行了调查研究，结果发现，在拟蕨类植物中，孢子体只存在兼性VA菌根营养和自养两种菌根营养类型，两种类型，两种类型所占的比例相当；在真蕨类植物中，厚囊蕨类植物VA菌根营养的比例较高（92%），且无自养类型；而在薄囊蕨类植物中，自养型占71%，VA菌根营养和兼性菌根营养所占的比例都不高，分别是15%和14%。

To improve spinosyn-producing strain and enhance spinosyns yield, we studied the effects of glycin concentration and the operational time, temperature and lysozyme concentration on protoplast preparation of Saccharopolyspora spinosa SP06081. We also studied different regeneration media and osmotic stabilizing agents.

为了改良多杀菌素生产菌种，提高多杀菌素产量，研究了甘氨酸添加浓度、溶菌酶作用时间、温度和浓度对多杀菌素生产菌刺糖多胞菌Saccharopolyspora spinosa SP06081菌株原生质体制备和再生的影响，并考察了不同再生培养基和渗透压稳定剂对其再生的影响，确定了该菌株原生质体制备和再生的最佳条件。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。