菌体

The extracting coenzyme Q_(10) from Blakeslea trispora using saponific ation was also studied by orthogonal test. And the optimum extracting conditions were obtained as follows: Wet culture was distilled at 70℃for 60min, added caustic potash up to 1.9 times of dry culture, pyrogallol up to 1/2 dry culture, and the concentration of alkali-methanol was 10%.The average recovery of CoQ_(10) was 81.5%.

采用正交实验，对醇碱皂化法提取三孢布拉氏霉菌体中辅酶Q_(10)的各工艺条件进行了研究，确定最佳的工艺条件为： KOH加量为干菌体质量的1.9倍，醇碱浓度为10%，焦性没食子酸添加量为干菌体质量的1/2，在70℃下皂化60min，其平均提取收率达81.5%。

The results showed that the bacteria B7 came into the logarithmic growth phase for 5 h culture. Both the fermentation liquid and its filtration could inhibit C. colhounii effectively, and the fermentation of dilution l0 times also showed significant differences compared with the control. The inhibiting effect of its fermentation media filtration was even more significant in the optimizing test, with the maximal inhibition rate of 100%. The best fermentation condition was 0.5% glucose, 0.1% ammonium muriate, without extra mineral, pH 5.4, temperature 28℃. Based on the morphological properties and 16S rRNA sequence, the bacterium B7 was identified as the Bacillus subtilis.

结果表明，该菌体在培养了5h后即进入对数生长期；B7细菌的发酵液原液和无菌滤液均能抑制病原菌的生长，其中发酵液原液的10^3稀释液的抑菌效果仍与对照之间存在显著的差异；发酵条件优化试验中发现该细菌的无菌滤液最大抑菌率达100%，最佳发酵条件为葡萄糖0.5%、氯化钱0.1%、不加无机盐、pH5.4，温度28℃；结合形态学特征和16S rRNA的序列分析，将该菌株鉴定为枯草芽孢杆菌。

In this study, we analyzed the compound of mycelium and used for reference of other researchers, a path of comprehensive utilization was proposed.

本研究从废菌体的化学成分分析入手，借鉴国内外的其它发酵行业的废菌体利用的经验，找出衣康酸废菌丝体的综合利用的途径。

By the observation under the transmission electron microscope, it was founded that Nisin extract made the cell distorted and plasmolysis, followed by cell broken and cell components releasing which turned the cell into irregular residues.

透射电镜观察发现，加入乳酸链球菌素后，菌体变形，出现质壁分离现象，进而菌体细胞破裂，细胞内容物大量外泄，菌体成为不规则残片。

Mature mycelial is thicker, have branch, the makes by deposit of fragment of actinomyces bacterium body, necrotic organization, calcic salt sulfureous grain that the model appears all round, grain brim has the small system of expand, the exterior appears great weight state, show radiative shape to arrange, friend says " actinomyces ".

成熟的菌丝较粗，有分支，四周出现典型的由放线菌菌体、坏死组织碎片、钙盐沉积构成的硫磺颗粒，颗粒边缘有膨大的小体，外观似棒锤状，呈放射状排列，故称"放线菌"。

Methods Assay the morphology of Penicilliosis marneffei in bone marrow, and compare with Histoplasma capsulatum.

分析骨髓涂片中马尔尼菲青霉菌的菌体形态特点，并与组织胞浆菌的菌体形态对比。

As known as traditional viewpoint, microbe could produce hydrogen continuously only when microbic cell is fixed, so present tests of this process offen adopt this process fixed-cell technology of pure microbe and were in the scale of batch fermentation test. Now the larggest ability of bio-producing hydrogen was 20mLH〓/g biomass·h produced by Rhodospirillum rubrum and 1. 6mLH〓/g biomass·h produced by Clostridium butyricum .

传统观点认为，微生物惟有进行细胞固定化才能达到持续产氢，所以现有试验常采用纯种细胞固定化技术，且尚处于分批培养试验阶段，最大产氢能力深红螺菌为20mLH〓/g菌体·h，丁酸梭状芽孢杆菌为1.6mLH〓/g菌体·h。

Results showed that, pyrolin had obvious action on two species of fungi, and the antifungal effect was positively correlative with concentration. The EC50 was 0.4076 mg/mL and 0.4626 mg/mL respectively after cultured 3 days. The main antifungal period was the fast growth period of fungi, and lead to it lag behind. Observation under SEM revealed that pyrolin caused a series of marked morphological and structural alterations of hyphal. These changes included mutual adhesion of mycelium, the hyphal was irregular in diameter, local denting, some of mycelium was broken, disruption of cell wall, outbreaking of inner cytoplast, and the empty cavity appeared. As a result, pyrolin restrained the growth of these fungi.

结果表明，鹿蹄草素对上述两种真菌均具有明显的抑制作用，抑菌效果与浓度呈正相关，培养3d时的EC50分别为0.4076、0.4626 mg/mL；抑菌时期主要是在真菌的快速生长期，使其快速生长期滞后；扫描电镜显示，鹿蹄草素作用下菌体形态发生异常变化，表现为菌丝体之间相互粘连，菌丝粗细不均匀，细胞壁破裂，局部凹陷，部分菌丝断裂，大量内容物外溢，出现空腔等现象，从而发挥其抑菌作用。

Wistar rats were fed with a high lipid diet supplemented with living or thermal death bacteria of Lactobacillus acidophilus MG2-1 which was isolated from koumiss in Mongolia and was of good ability of acid tolerance and decreasing the level of cholesterol in vitro.

利用从蒙古国酸马奶中分离鉴定，并通过耐酸性及体外降胆固醇能力测试筛选获得的L.acidophilus MG2-1菌制备成活菌体和热致死菌体制剂与高脂饲料同时灌喂Wistar系大鼠，研究探讨了对其血清脂质代谢的影响。

To obtain high cell density and high yield of MTG by Streptomyces hygroscopicus, the effects of exponential feeding of glucose and the addition of nitrogen source on MTG production were investigated.

在吸水链霉菌分批发酵研究的基础上，通过在菌体生长阶段指数流加葡萄糖，进行高细胞密度培养，获得了较高的菌体量；待菌体生长进入产酶期后，通过补加氮源，为产酶提供充足的氮源，其中通过流加蛋白质氮源，可以减少蛋白酶对成熟MTG的分解，促进产酶。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。