菌体

The third fluroquino is one kind of antibiotics which is often used in ophthalmology. It's antibacterial activity for Gram-negative bacteria is better than that for Gram-positive bacteria and it is the weakest for anaerobe and endo-pathogens.

第三代氟喹诺酮类药物是眼科常用的抗菌药物，它对革兰氏阴性菌有良好的抗菌活性，但对革兰氏阳性菌(如肺炎球菌、化脓性链球菌、肠球菌)的抗菌作用相对较弱，对厌氧菌和胞内病原体(衣原体、支原体、军团菌、分枝杆菌)作用较差。

The third fluroquino is one kind of antibiotics which is often used in ophthalmology. Its antibacterial activity for Gram-negative bacteria is better than that for Gram-positive bacteria and it is the weakest for anaerobe and endo-pathogens.

第三代氟喹诺酮类药物是眼科常用的抗菌药物，它对革兰氏阴性菌有良好的抗菌活性，但对革兰氏阳性菌(如肺炎球菌、化脓性链球菌、肠球菌)的抗菌作用相对较弱，对厌氧菌和胞内病原体(衣原体、支原体、军团菌、分枝杆菌)作用较差。

Along with the fluconazole solution density rise,the experimental two kind of strain various glucose density is higher,showe d the glucose consumption are less,takes the logarithmof the medicine de nsity,discovered the logarithm the medicine density and each glucose den sity presents the linear relations;Carries on the analysis comparison to under the fluconazole function two kind of strain linear relations,disc overed the relations of the two strains has the nonuniformity.3 Compare the fluconazole induction reaiatance SC5314 strain and sens itive strain compares,its difference gene expression mainly concentrates in:The code proteinase body and the protein hydroltyic enzyme gene,in the code sugar fat metabolism process is connected the protein gene,the cell cycle correlation gene,the duplication and the translation adjustme nt correlation gene,the stress response correlation gene,the line plast ochondria correlation gene,the cell wall function related gene.4 Candida albicans SC5314 induction resiatance strain was processed b y Xianglian solution,its expression change gene mainly is:Code stress re sponse family protein gene,biomembrane relevant gene,a code proteinase body gene race,code cell cycle related protein gene,duplication and tra nslation adjustment related protein gene.5 The clinical reaiatance strain Candida albicans was processed by Xi anglian solution,its expression change gene mainly is:Codes the hot sho ck protein gene,the serine/threonine protein activating enzyme gene,the proteinase body family gene,the regulation copies and translates the ge ne.

随着氟康唑药液的浓度上升，试验的两种菌株各孔葡萄糖浓度越高，说明葡萄糖消耗越少，经过药物浓度取对数后进行分析，发现取对数后的药物浓度和每孔中葡萄糖浓度者呈现线性关系；对氟康唑作用下的两种菌株的线性关系进行分析比较，发现对两种菌株作用具有不一致性。3氟康唑诱导的耐药SC5314菌株与诱导前的敏感株相比，其差异基因表达主要集中在：编码蛋白酶体及蛋白水解酶的基因，编码糖脂代谢过程中相关蛋白的基因，细胞周期相关基因，转录及翻译调节相关基因，应激反应相关基因，线粒体相关基因，细胞壁功能相关基因。4白念珠菌SC5314诱导耐药株经香莲外洗液作用后，其表达变化的基因主要是：编码应激反应家族蛋白的基因，生物膜相关性基因，编码蛋白酶体基因一族，编码细胞周期相关蛋白基因，转录及翻译调节的相关蛋白基因。5白念珠菌临床耐药菌株经香莲外洗液作用后，其表达变化的基因主要是：编码热休克蛋白基因，丝氨酸/苏氨酸蛋白激酶基因，蛋白酶体家族基因，调控转录及翻译基因。

The results showed that:The best zymolysis conditions for BS and TV protoplasts release and regeneration through orthogonal experiment were as follows: 1mg·mL~(-1) lysozyme treated BS protoplasts for 1.5 hours at 37℃ and 4 mg·mL~(-1) lywallzyme treated TV protoplasts for 2 hours at 30℃. In these conditions, the number of protoplasts release was respectively 8.87×10~8 units/mL and 8.62×10~7units/mL and the regeneration rate was respectively 54.1% and 29.3%.

研究结果表明：通过正交试验确定BS菌和TV菌原生质体制备过程中的最佳酶解条件分别为：BS菌在37℃酶解温度下，1mg/mL溶菌酶酶解1.5h，原生质体制备个数达到8.87×10~8个/mL，再生率为54.1%；TV菌在30℃酶解温度下，4mg/mL破壁酶酶解2h，原生质体制备个数为8.62×10~7个/mL，再生率为29.3%。

The fungicidal activity of methanol extract was higher than that of ethyl acetate extract, and the inhibition rates were higher than 90% against Alternaria longipes, Fusarium oxysporium, Gibberella zeae, Glomerella cingulata, Exserohilum turcicum under the concentration of 1000g/mL The results of the inhibition of spore germination indicated that the fermentation products exhibited obvious inhibition rate against Alternaria longipes, Exserohilum turcicum and Fusarium coeruleum, the EC50 values was 59.4g/mL, 96.5g/mL and 132.5g/mL. In the bioassay of the fermentation products against Bacillus sub tills and Bacillus cereus, by means of detection fo inhibition zone, the inhibition zone diameter was 20mm and 27mm. The results of pot tests that methanol extract showed higher than 70% of protective efficacy and therapeutic efficacy against wheat powdery mildew under the concentration of 2000g/mL.

其中，甲醇提取物的抑菌活性比乙酸乙酯提取物强，在1000g/mL浓度下对烟草赤星病菌、马铃薯干腐病菌、小麦赤霉病菌、苹果炭疽病菌、玉米大斑病菌的菌丝生长抑制率均大于90%；抑制孢子萌发法试验结果表明，2K3菌株菌丝体的甲醇提取物对烟草赤星病菌玉米大斑病菌和马铃薯干腐病菌的EC50分别为59.4g/mL、96.5g/mL和132.5g/mL；管碟法生测结果表明，2K3菌株菌丝体的甲醇提取物对枯草芽孢杆菌和蜡状芽孢杆菌的抑菌圈直径分别为20mm和27mm；盆栽试验结果表明，2K3菌株菌丝体的甲醇提取物在2000g/mL浓度下对小麦白粉病的治疗和保护作用均在70%以上。

In this paper, 1554 strains of bacteria were isolated from the soybean rhizophere in the different continuous systems. Those biocontrol bacteria were screened in vitro and in vivo. 6 strains of them had the better control efficacy. They exhibited the different antagonism to the pathogens of soybean root in vitro. The strains 18BR2-2 and 18BR9-1 showed a wide range of antagonism against Fusarium oxysporum, F. avenaoeum, F. solani and Rhizoctonia solani in vitro. The 6 strains exhibited different control efficacy to soybean root rot in greenhouse and in field. In greenhouse, they could decrease the degree of soybean disease significantly, and the control efficacy of 18BR2-2 was the best and can reach 45.90%. In theory, soybean treated by 18BR2-2 may postpone the days of outbreaking of disease for 6 days. The strain 18BR2-2 also promoted the growth of soybean seedling and, raising the soybean height for 11.24%, increasing dry weight for 40.91% and fresh weight for 35.27%.

本研究从长期连作田种植的大豆及其根际土壤中分离细菌1554株，采用平板测试、温室盆栽及田间试验等方法，筛选了对大豆根腐病菌(主要是尖孢镰孢菌Fusarium oxysporum)有很好作用效果的生防细菌6株，在离体的条件下均表现出不同程度的拮抗作用，其中生防细菌18BR2-2和18BR9-1表现出很广的杀菌谱，对大豆根腐病菌尖孢镰孢菌(F.oxysporum)、燕麦镰孢菌(F.avenaoeum)、茄腐镰孢菌和立枯丝核菌都有很好的离体拮抗作用，并且这6株生防细菌在盆栽和田间的情况下均表现出对大豆根腐病一定的防治效果。

Results of non-reducing sodium dodecyl sulphate polyacrylamide gel electrophoresis of the supernatant and aggregate precipitate formed in refolding process show that except being refolded to native egg white lysozymes, the reduced-denatured lysozymes can also form the aggregates with molecular weights being separately about 30.0 and 35.0 kD, while the reducing SDS-PAGE and the refolding results in the presence of sodium dodecyl sulphate show that these aggregates are formed chiefly through the misconnection of disulfide bonds between the reduced-denatured lysozymes, and the aggregate precipitates are formed through the non-covalent interactions between the aggregates with molecular weight being about 30.0 kD.

复性过程形成的上清和集聚体沉淀的非还原电泳结果表明，除了能复性成天然态的溶菌酶分子外，还原变性蛋白溶菌酶同时还能形成分子量分别约为30.0KD和35.0KD的蛋白溶菌酶分子集聚体；而它们的还原电泳和在SDS存在时的复性结果表明，这些集聚体主要是通过还原变性蛋白溶菌酶分子间的二硫键错配而形成，而集聚体沉淀则是通过分子量约为30.0KD的集聚体分子之间的非共价相互作用而形成。

In this thesis, Chlortetracycline fermentation process was studied systematically.

作为一类重要的医药产品，抗生素一般是由微生物产生的次级代谢产物，在其生产过程中的菌体生理特性和过程优化就显得特别重要，并成为研究的主要对象。

The effect of aeration rate was studied in the culture of brewer's yeast used for ATP production.

结合酵母细胞酶促合成ATP的活性测试，在5L发酵罐中培养啤酒酵母，考察了通气对菌体生长和细胞活性的影响。

A related organism, Bacillus popilliae, is used to combat the Japanese beetle. This bacterium, however, cannot be grown in fermenters, and inocula must be grown in the living host.

一种相关微生物——日本甲虫芽孢杆菌，被用来杀灭日本甲虫，但这种细菌不能在发酵罐中生长，接种菌体必须在活体中生长。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。