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According to the above results, feather, tryptone, yeast extract as well as incubation time and temperature were selected for further studies by RSM. Data obtained showed the maximal biomass production and keratinase activity occurred at above 96 h.

根据上述结果选择feather、tryptone、yeast extract外加两个物理因子进行第一次的RSM分析,结果发现欲得到较高的角蛋白酶活性与菌体产量,其培养时间需超过四天以上。

Significant interactions between pH and salinity, as well as pH and temperature were found during the germination respectively, otherwise the relationship between salinity and temperature was relatively unapparent.

结果表明, VPJ33的最优培养条件为:pH 8.41、温度34.1℃和盐度2.47%;菌体生长过程中, pH和盐度以及pH和温度对VPJ33生长的交互作用显著,盐度和温度对VPJ33生长的交互作用不显著。

Fluorescence staining with uranine and aniline blue generated yellow-green fluorescence in the fungal structures under UV or blue light, and the black background of host tissues gave rise to strong contrast for observation.

荧光素钠和苯胺蓝两种荧光染色方法均能使菌体在紫外或者蓝光下产生黄绿色荧光,而寄主组织呈现黑色背景,强烈的反差利于观察。

Crude enzyme liquid alone had no function in decolorization, while mycelium had great function in decolorization. When added H2O2 and veratryl alcohol into the crude enzyme liquid, the decolorization rate had increased from 0% to more than 30%, so the H2O2 and veratryl alcohol played roles in the decolorization.

在利用粗酶液废水脱色研究时,发现粗酶液单独没有脱色作用,有菌体存在时脱色效果明显:粗酶液中加人过氧化氢和黎芦醇后,脱色率由0%增至30%以上,,因此酶与染料作用过程中也需要过氧化氢和黎芦醇作为中间体。

Chang An medical stone contains a lot of trace elements and adsorbs or destroys many kinds of hazardous matter and viruses,favourably adjusting pH value.

长安麦饭石含众多的微量元素,对有害物质和多种菌体具有强烈的吸咐作用,对 pH 值有双向调节作用。

Studies on mechanism of Biosorption and toxicity of chromium indicated that Biosorption was contained of surface adsorption, transport across cell wall and membrane, and bioaccumulation. Further more, bioaccumulation played significant role in total Biosorption capacity, it attainted 70.6%. And the resistance on chromium of cell wall was perfect, breakage exceeded of 0.1 nm was not conduced by 300 mg/L chromium. Detoxifcation ability would be facilitated by activated sludge.

对吸附机理和毒性的初步探讨结果表明,菌体对铬的吸附由表面吸附、跨壁膜运输和体内积累组成,其中体内积累对吸附的贡献值最大,达到总吸附量的70.6%;细胞壁对铬毒性的抗性理想,300mg/L铬不会对细胞壁产生0.1nm以上的形态破坏;活性污泥的联合使用有助于铬的还原。

It showed that dissolved oxygen concentration and aeration time were important factors which influenced the growth of ceils and the ethanol fermentation intensity.

结果表明,溶氧浓度和通气时间是影响菌体生长和乙醇发酵强度的重要因素。

Chlorine dioxide etc and organic chlorinates are introduced briefly. Some characteristic properties of commercial oxidative bactericides are investigated experimentally bactericidal efficiency, colloid breaking down and ferrous/ferric ions removal.

本文以室内对比试验结果展示了氧化型杀菌剂的特点:作用快、杀菌致死时间短,有破胶聚沉和除铁作用,有一定腐蚀性,有清除菌体堵塞作用,不能用于高pII值环境。

The invention uses the fermentation liquor of one Escherichia coli generating polysialic acid as material, filters and removes thallus, adds some solvents as sodium chloride and alcohol into supernatant to deposit polysialic acid, dissolves the polysialic acid deposits via deionized water, filters to remove modified protein, uses alkali prolease to treat filtrate, complexes deposits via cetyl pyridinium chloride or cetyltrimethylammonium bromide, and dissociates, uses over alcohol to deposit polysialic acid, dissolves the deposits via ultra-pure water, grades and purifies via chromatography column, collects and dialyzes eluent, cools and dries to obtain polysialic acid.

本发明以一株产聚唾液酸大肠杆菌的发酵液为原料,经过过滤去除菌体,上清液加入适量氯化钠和乙醇等溶剂沉淀出聚唾液酸,聚唾液酸沉淀用去离子水溶解,再用过滤去除变性蛋白质,滤液经过碱性蛋白酶处理、氯代十六烷基吡啶或十六烷基三甲基溴化铵络合沉淀并解离后,再用过量乙醇沉淀出聚唾液酸,聚唾液酸沉淀用超纯水溶解,用层析柱进行分级纯化,收集洗脱液经过透析,冷冻干燥得聚唾液酸产品。

As determined by SDS-PAGE and dcnsitometry, the overproduced proteins comprised up to 18.5% of the total bacterial protein.

通过SDS聚丙烯酰胺凝胶电泳检测表明,在实验室规模的发酵实验中,NAP-1/IL-8在大肠杆菌中的表达量可占菌体可溶性蛋白的10.9%;而在摇瓶培养中则占18.5%。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

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