菌体

Inoculate a single colony in BMGY medium, grow at 30 °C with 250 r/min until culture reatch an OD6oo=2.0. Harvest the cells by centrifuging at 1,500 r/min for 5 min at room temperature. Resuspend cell pellet to an OD6oo=10 in BMMY medium to start induction.

将经过酶切鉴定和测序正确的阳性克隆电转入毕赤酵母GS115中，挑取酵母转化单菌落，接种于BMGY培养基，30℃、250r/min培养至OD_(600)=2.0时室温1，500r/min离心5min，用BMMY培养基重悬菌体使OD_(600)=1.0，重新放入摇床进行诱导。

The thalli were short poleour and the wide white ringe and narrow white ringe were observed when it was cultured in TZC medium.

菌体短杆状，在TZC选择性培养基上培养出现白边较宽和白边较窄的两种菌落。

Objective. To determine if viral vectors carrying the gene for brain-derived neurotrophic factor could be used to promote an axonal regenerative response in rubrospinal neurons after an acute cervical spinal cord injury.

目标：本研究在于确定病毒带菌体存在着一种大脑源性神经营养因子的基因，这种基因可以在急性颈髓脊索损伤所致病例中引发红核脊索神经元的轴性再生反应。

Objectie. To determine if iral ectors carrying the gene for brain-deried neurotrophic factor could be used to promote an axonal regeneratie response in rubrospinal neurons after an acute cerical spinal cord injury.

目标：本研究在于确定病毒带菌体存在着一种大脑源性神经营养因子的基因，这种基因可以在急性颈髓脊索损伤所致病例中引发红核脊索神经元的轴性再生反应。

Furthermore, the control efficacy of the extract product of mycelium and the fermentation extraction were all 100% against Sclerotinia sclerotiorum under the concentration of 10 mg/mL.

菌丝生长速率法试验结果表明，在1 mg/mL浓度下，菌体提取液对8种供试植物病原真菌的菌丝生长抑制率大于90 %；发酵液提取物对11种供试植物病原真菌的菌丝生长抑制率大于90 %。

Yellow serofluid, brown yellow in color and colloid turbid state in appearance, contained sugars, ethanol, solids, and especially rich alcohol, aldehyde, acids, esters and beneficial microbes. It could be used as precursory substance for the improvement of liquor quality and liquor taste and the synthesis of flavoring substances.

黄浆水为棕黄色、胶体混浊态，含有糖分、酒精、固形物，特别是含有大量的醇、醛、酸、酯及有益微生物菌体，是可用来提高曲酒质量、改善风味、合成香味物质的前驱物质。

Commassie brilliant blue staining can clearly display the different structures of Sphaerotheca fuliginea, such as germinating conidia, initial germtubes, hyphae and conidiophores, and hardly stain the host tissue, presenting a striking contrast between fungus and host tissue.

考马斯亮蓝组织透明染色方法可以清楚观察到白粉病菌的5个发育阶段，即萌发的分生孢子、初生芽管、胞间菌丝、分生孢子梗和后期菌落。考马斯亮蓝几乎使寄主组织不着色，不产生背景色干扰，而菌体变深蓝色。

Thalli of the algae were separated from the culture medium by gentle filtration.

菌体的藻类分离培养基的温柔过滤。

Not long time later, due to the continuous migration and not-stop reproduction, the microbial thalli were distributed widely in the confine between the initial places and the oil-water interface.

可视化实验表明，由于不断的运移和不停的繁殖，在微生物初始位置与油水界面之间的范围内，广泛地分布着微生物菌体。

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2～3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12，在酵母细胞中实现了多拷贝强表达；荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液，测定510 nm处的荧光发射强度结果显示，表达的绿色荧光蛋白对氧化还原水平敏感，且在510 nm处的荧光强度与一定的重金属浓度呈正相关，即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强，从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用；同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属；遗传学的点突变频率及致死率实验数据表明，重金属能导致菌体的点突变频率和致死率升高，且活性氧的清除剂巯基脲能明显降低这种点突变和致死率，说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。