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Researchers injected a vector designed to return CREB production to normal in mice that had been genetically modified to underproduce the protein.

研究者将一种改造过的带菌体注入经过基因改造而致使体内CREB含量不足的老鼠体内,使其含量能恢复正常。

As the final results, the harvesting wet cell density——186g/L in the fermentation broth and the ELISA units of preS1 antigenicity in the cell——8 000 after 45h main culture were obtained.

实验获得主培养45h后湿菌体浓度为186g/L,细胞内preS1效价为8 000u。

An primary optimization for medium composition in shake flask showed the best carbon source, nitrogen source , culture volume , incubating quantity and pH after having completed studying the relation between thalli form,zymotic condition and metabolized yields .

通过摇瓶培养,研究发酵条件对菌体形态、产物产量的影响,初步优化了发酵培养基组成,确定了最佳碳源、氮源、装液量、接种量、pH等。

The addition of glucose and (NH4)2SO4 could greatly enhance the degradation rate of ABAS by the strain.

葡萄糖和硫酸铵的加入显著地提高菌体降解溴胺酸的速度。

The results of statistic property theory analysis and simulation experiment show that CABGM replicated penicillin batch fermentation biomass growth process described by dynamic differential equation model accordantly.

对CABGM进行了统计特性的理论分析和仿真实验,理论分析和仿真实验结果均证明了CABGM能一致地复现动力学微分方程模型所描述的青霉素分批发酵菌体生长过程。

During the contact the aminophenol composition changes gradually.

在与大肠杆菌的作用过程中,菌体蛋白组成发生明显的变化,游离氨基酸总量增加。

Polysaccharides extracted from ultramicro-bacteria Glaciecola polaris on immune and anti-stress ability in Chinese soft-shelled turtle stressed by pH were investigated. The results showed that the significant increases (P.01) were observed at the phagocytic rate of blood cells, serum bacteriolytic activity and bactericidal bacteriolytic activity in polysaccharide groups.

选用平均体重50-60g的中华鳖幼鳖300只,随机分成10组,试验组在基础饲料中添加0.05 %、0.1 %的超微细菌Glaciecola polaris的蛋白多糖,或添加0.1 %、0.5 %的超微细菌菌体粉碎物,对照组以0.5 %a-淀粉代替。

In comparison,the rhizobia in mature infected cells were larger,greater in number,varied in shape,and distributed throughout the nodule cells.The rhizobia in the mature cells also had poly-hydroxy butanoic acid and curapos.

成熟侵染细胞中,根瘤菌个体较大,数量较多,多呈棒状,少数为球形或椭球形,有很多根瘤菌还呈现明显的&T&形、&Y&形或&V&形,菌体占满了整个细胞,这时的根瘤菌大多数含有聚羟基丁酸和聚磷酸盐颗粒。

The slight reduction in Act productivity with lyqSR2005 showed the Y -butyrolactone binding protein was a weak positive regulator. From above results, it seems that ScbR/ScbA genes play an important role in the self-regulation system in S.

必和ajl侧刀d厂尹这两个基因是在菌体对数生长期到一平缓期的过渡期开始表达,而在平缓期大量表达;固体培养基中的空间表达模式表明,厂。

We reconstructed the gene of human scFv and prepared scFv and scFv antibody fragments by prokaryotic expression system PET22b/BL21(DE3). After being purification by Ni-NTA column and renaturalization by urea dialyzation, the affinity and thermostability of scFv and scFv were detected.

结果表明,成功构建了scFv基因;实现了scFv和scFv在原核中的高效表达,二者的表达量均占菌体总量的35%以上,且成功的纯化了表达的重组蛋白,复性后获得了有活性的纯化重组蛋白。

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