菌体

In the experiment, compared to atmosphere distillation, low-pressure distillation is proved to be able to save more nourishment, remain more yeast for the fermentation with cornstalk and finally give more energy saving.

与常压精馏相比较，低压低温精馏能够减少醪糟中营养物质的损失，更多地保存了酶活力和发酵菌体的活性，为醪糟进行下一步的秸秆固态发酵饲料生产提供保证；而且有利于进行浓醪精馏，实现大幅度的节能。

Gold Baby ferment can be used to feed a variety of energy fermented feed and fodder, such as rice powder, corn flour, wheat bran, rice bran and straw crops, Cotton Seed Hull and grass and so on, after the fermented feed energy is transformed into the bacterial protein feed , which is transformed into the roughage feed.

金宝贝饲料发酵剂可用于发酵各种能量饲料和粗饲料，如稻谷粉、玉米粉、麦麸、米糠及农作物秸杆、棉籽壳和牧草等，经发酵后的能量饲料转化成了菌体蛋白饲料，而粗饲料转化成了精饲料。

By means of polymerase chain reactionand DNA recombinant techniques,the gene encoding mature ouse IL-4 was obtained from plasmid pCD-mIL4 and was inserted into expressionvector pBV220,which contains _RP_L double promoters,synthesized SD sequence and temperaturesensitive CIts857 gene,thus an expression lone eferred as pBV-mIL4 was constructed.

该质粒含有 P_RP_L 串联启动子，合成 SD 序列和温度敏感的CIts857基因，并且去除了完整小鼠 IL—4 cDNA 中3′侧翼非编码区，以原核系统高频使用的强终止密码子TAA 代替了原小鼠 IL-4基因的终止密码子 TAG，经 SDS-PAGE 及凝胶密度扫描分析，表达的重组小鼠 IL-4分子量约为14.2KDa，占总菌体蛋白量的25～30%。

The final acidity of yogurt ferment by the two bacteria was 57.65 T and 54.43 T respectively.Because the -galactosidase produce by starter is a endoenzyme it must abstract after the cell wall broken. This experiment compare the different method of cell wall broken and research on the influence of temperature and time on condition of cell wall broken.

由于发酵剂菌种产生的糖代谢酶，β-半乳糖苷酶是胞内酶，通过对渗透压破壁和不同浓度的氯仿和甲苯两种有机溶剂破壁方法的比较，以及破壁所需温度和时间的研究，确定采用5％氯仿，45℃保温8～12h对菌体进行破壁，β-半乳糖苷酶的活力采用AOAC提出的标准进行测定。

Its mycelial is equal to the root of plants; its thalli are bole and branch of plants; its spore is plants' farina and seed.

它的菌丝相当于植物的根；菌体相当于植物的干和枝；孢子相当于植物的花粉和种子。

Potassium ferricyanide and dithiothreitol were used to control redox potential from －100 mV to －450 mV in 3 L fermenter.

在3 L发酵罐上利用铁氰化钾和二硫苏糖醇调节发酵体系氧化还原电位值在－100～－450 mV，结果表明－350 mV为菌体生长和产丁二酸的最佳电位，丁二酸生产速率由0.75 g/提高到1.18 g/，产物丁二酸与副产物乙酸的质量浓度比由2.5提高到3.9。

An astaxanthin overproducing strain was obtained by mutagenesis. in 200l common stirring fomenter, 60mg/l - 70mg/l astaxanthin were harvested by fed-batch cultivation of phaffia rhodozyma. results of optimization of extract condition indicated astaxanthin productivity was 1.14mg/g by acid methods under optimal condition. this achievement reached to the leading level of the field in china, and also advanced method internationally. in the same time, it is a good basis for the further research for the fermentation of natural astaxanthin.

分离选育出虾青素高产的海洋红酵母，在200l中试罐中发酵虾青素，虾青素的产率达60-75mg/l发酵液；对天然虾青素的无毒提取技术进行研究，虾青素的提取得率为1.14 mg/g干菌体；虾青素发酵水平和提取技术水平为国际先进，国内领先，为用发酵法生产天然虾青素打下坚实的基础。

An astaxanthin overproducing strain was obtained by mutagenesis.In 200L common stirring fomenter, 60mg/L - 70mg/L astaxanthin were harvested by fed-batch cultivation of Phaffia rhodozyma. Results of optimization of extract condition indicated astaxanthin productivity was 1.14mg/g by acid methods under optimal condition. This achievement reached to the leadinglevel of the field in China, and also advanced method internationally. In the same time, it is a good basis for the further research for the fermentation of natural astaxanthin.

分离选育出虾青素高产的海洋红酵母，在200L中试罐中发酵虾青素，虾青素的产率达60-75mg/L发酵液；对天然虾青素的无毒提取技术进行研究，虾青素的提取得率为1.14 mg/g干菌体；虾青素发酵水平和提取技术水平为国际先进，国内领先，为用发酵法生产天然虾青素打下坚实的基础。

After centrifugation, broth culture precipitates were smeared and stained with fuchsin to observe the morphology change in thallus under optical microscope.

离心肉汤培养物，取沉淀涂片复红染色，光镜下观察菌体形态变化。

It could be suggested that the glyceride biosurfactant was produced by C. antarctica at early stage for cell adapting the hydrophobic environment., and the alkane would be emulsified with the biosurfactant and made easy to be attached and up taken by cell.

由此可以推测，C.antarctica在正构烷烃中产生生物表面活性剂是在发酵前期，可能是菌体为了适应不良的生存环境而产生生物表面活性剂以乳化烷烃，增加与烷烃的接触能力，最终达到利用烷烃的目的。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐