菌体

We studied the effect of acetic acid on cell growth and recombinant porcine somatotropin for recombinant Escherichia coil.

在这里我们所探讨的是醋酸对重组大肠杆菌的菌体生长和重组蛋白质猪生长激素表现度的影响。

It was concluded that the electrostatic attraction between negative dye molecule and positive biomass due to amidogen protonation was the important mechanism of dye biosorption by biomass.

氨基质子化引起的菌体正电性和染料负电性之间的静电引力是染料吸附的重要机制。

The methylated amidogen still held protonation at low pH. Therefore, the adsorption ability of biomass was almost unchangeable through methylation of amidogen in biomass. The acetylated amidogen lost protonation at low pH, and the capacity of dye adsorption of biomass decreased by 51.6% after acetylation of amidogen in biomass.

甲基化氨基在酸性条件下仍然可以质子化，故氨基甲基化修饰后染料吸附性能不变；乙酰化氨基在酸性体系中失去质子化能力，乙酰化修饰菌体染料吸附性能下降51.6%。

EVA1, a thermophiliccellulolytic anaerobe, to insoluble cellulose was reported.

EVA1在降解纤维素的过程中，菌体对不溶性纤维素底物具有强烈的粘附作用。

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

Finally, the fusion protein was expressed in E coli DH5α under induction by arabinose. And the fusion protein was 65 kD and account for 30% of the total protein of the bacteria.

SDS-PAGE电泳分析显示，融合蛋白分子量约65 kD，与预期蛋白分子量一致，并且目的蛋白占菌体蛋白的30%。

The P1 recombinant protein extracted and purified with B-PER GST Fusion Protein purification Kit was used as an antigen, and thallus component of full Mp was used as an antigen control to establish indirect ELISA experimental method to test and determine IgG antibody of serum samples from 40 normal blood donors and 51 clinical serum of partly certain and partly uncertain Mp infector.

以GST融合蛋白层析柱提取、纯化Mp P1重组蛋白做抗原，以全肺炎支原体菌体成分做抗原对照，建立间接ELISA实验方法，检测40份正常献血者血清标本和51份疑似MP感染临床血清标本的IgG抗体。

The miolphology of cells and protoplast is observed with electron microscope.

用电镜观察了菌体和原生质体的形态结构。

Methods Split the recombinant E. coli for expression of rhOP-1 by ultrasomication in ice bath and extract the inclusion body. Dissolve the inclusion body with 8mol/L urea, purify by SP-Sepharose chromatography, refold by gradient dialysis.

将表达rhOP-1的大肠杆菌菌体在冰浴下超声裂解，分离提取包涵体，用8mol/L尿素溶解，纯化后进行梯度透析复性。

Suis was found in all identifiable crypt areas; SS2 also appeard in marginal zone and periarterial lymphoid sheath of spleen, hepatic sinusoid of liver, interstitium of lung; presence of SS2 was at the cortex where histopathological lesions and expression of inflammatory mediators were observed.

实验表明，SS2主要定殖于扁桃体的隐窝附近；在脾的边缘区和动脉周围淋巴鞘附近、淋巴结的生发中心和皮质内、肝血窦、肺间质和肾小体中均可见菌体状荧光颗粒；在脑组织中发生脑膜炎的区域，如皮层等可检出细菌。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐