菌体

Hydroxysteroid dehydrogenase/carbonyl reductase ( 3α-HSD/CR,EC 1.1.1.50 ) from Comamonas testosteroni is an inducible enzyme which catalyzes the oxidation of androsterone with NAD+ to form androstanedione and NADH. 3α-HSD/CR was a member of the SDRs family, whose crystal structure had been solved by X-ray diffraction in 2000. The structure of 3α-HSD/CR is a homodimer which shows a typical pattern of Rossmann-fold structure consisting of βαβ units, and reaveals similarity with other members upon the SDRs family.

中文摘要 3α-Hydroxysteroid dehydrogenase/carbonyl reductase ( 3α-HSD/CR，EC 1.1.1.50 )是从Comamonas testosteroni菌体中分离而来的诱发性酵素，利用NAD+为辅因子，将受质androsterone氧化成androstanedione并伴随NADH的生成而完成催化反应。3α-HSD/CR是属於SDRs家族成员之一，其立体结构已经在2000年利用X-ray晶体绕射所解出。3α-HSD/CR在结构上为一同源双聚体，并和其它家族成员一样具有βαβ二级结构所叠合组成的Rossmann-fold特徵。

Activity of SOD, GSH-PX and content of MDA were examined in liver tissue homogenate and serum of rat after 28 d orally administrated with heat-killing and living k addophilus MG2-1, isolated from koumiss and could resist acid and artificial gastric and intestinal .juice.

应用分离自酸马奶，并经过耐酸性实验、人工胃肠消化液中存活能力筛选的L.acidophilus MG2-1，制备成热致死菌体和活菌制剂后灌服大鼠至28 d，测定大鼠肝脏组织匀浆和血清SOD，GSH-PX活力和MDA含量。

Secondly, for preparation of reference sera for serotyping, fifiteen serotypes of Haemophilus parasuis reference strains were cultured, harvested and inactivated prior to being emulsed with adjuvants for raising antibody in rabbits.

试验中，我们将引进的副猪嗜血杆菌15种血清型标准菌株在TSA培养基上增殖，收获菌体，灭活后用于免疫家兔，制备了分型血清。

Through the selection of culture medium and the mutant of mycelium that has a high production of PUPAS oil , the oil containing mycelium can be obtained with fermentation, and the dry mycelium contains 42.75挆47.78% oil.

本课题通过研究，选出了深黄被孢霉Mortierellaisabellina株作为摇瓶小试菌株，该株生长快，菌丝多，利用培养基原料能力强，易于形成孢子，生物量49g干菌体/L发酵液，含PUFAs46.61％，其中GLA2.3％。

This gene, together with a phytase gene ( 168phyA) identified in the B. subtilis strain 168 genome by a homology search, was cloned and over-expressed in B. subtilis using a phi105MU331 prophage vector system. Up to 35 units of phytase/ml were secreted into the culture media; and mature enzymes of around 44-47 kDa were purified for characterization.

借助于ф105MU331前噬菌体载体系统，这个基因和另一个通过同源性研究并在B.subtilis菌株168的基因组中被鉴定的植酸酶基因(168phyA)，于B.subtilis中得到克隆和过度表达。B.subtilis菌体向培养基中分泌了高达35个单位的酶夜，同时分子量在44-47kDa之间的成熟酶得到纯化。

The results showed that the cadmium-tolerant capability of the strain was different with antibiotics and the surface groups of the strain were affected by antibiotics in the presence of Cd2+.

结果表明，抗生素明显影响菌体的耐镉能力，菌体表面官能团在Cd2+和抗生素同时存在下，明显受抗生素作用的影响。

This strain could degrade DDT with degradation efficiency of 73.6% in 10 days.

对菌体降解DDT特性的研究表明，该菌株对DDT降解10d的降解率为73.6%。

Sterilization in desinfection chamber and subcultures of collections ;②confection of fermentation media and sterilization technics;③influences to objective produce altering the fermentable conditions ,including the examinations of the parameters about remnant sugar、pH、growth of microorganisms and produce;④separations and distillations of objective produces in fermented broth,the distillation technics of filter press and ion exchange;⑤brief and operation techniques of automatic fermentation tank.

课程内容：本课程主要实验内容：①微生物无菌室灭菌及种子扩大培养技术；②微生物发酵培养基及配制及灭菌技术；③微生物发酵及发酵条件的改变对目的产物代谢的影响，包括菌体生长、pH、残糖和产物等发酵过程中各项参数的检测；④发酵液中目的产物的分离及提纯，板框压滤离子交换提取技术；⑤自动发酵罐的简介及操作技术。

At first, the broth was acidified, then centrifuged to get mycelia, which was heated and powdered; finally we use acetic ether to soak the powder. After that, the phase of acetic ether was washed using the solution of ammonium sulfate and the solution of ethanedioic acid, then concentrated to gain pulps. It was crystallized at 4℃.The coarse crystals was decolorized by activated carbons, the pure needle crystal of lovastatin was obtained.

发酵液经酸化处理后，离心得到菌体，烘干后过筛得到菌粉；用乙酸乙酯浸泡，乙酸乙酯相需用(NH_4)_2SO_4溶液和草酸溶液洗涤两次；乙酸乙酯相经薄膜浓缩得到浆状物，于4℃结晶，得到粗晶体；再经粉末活性炭脱色和重结晶后得白色针状结晶，为lovastatin的纯品。

The result showed that the cell concentration was of stabilization when induced strains were incubated for 3 hours in the minimum medium,and mycostatin should be added after 4 hours and the inhibition finished after 10 hours in the MM with high nitrogen, in this way could good effect be achieved.

结果表明，诱变菌液在基本培养基中饥饿培养3 h后，菌体浓度趋于稳定；在高氮源培养基中培养4 h后加入制霉菌素，于10 h时结束其抑制作用，能取得较好的诱变效果。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。