菌体

Fed-batch fermentations of vitamin B12 by Propionibacterium shermanii were conducted in a 7L fermentor to investigate characteristics of the cell growth and vitamin B12 production.

首先对维生素B12产生菌Propionibacterium shermanii在7L发酵罐内分批发酵下的菌体生长及产维生素B12的特性进行了研究。

With ultrasonic wave and lysozyme, the recombination N protein is split from bacteria. We dissolve it in guanidine hydrochloride, purify it with Ni〓 affinity chromatography column and renature it in vitro. By testing the antigen of the purified N protein, it shows that its activity is very high.

建立了用超声波和溶菌酶裂解菌体，用盐酸胍溶解，Ni〓亲合层析柱纯化及体外复性等方法，经检测证明纯化的VSV重组核蛋白抗原具有较高的活性。

The results showed that the cells deformed severely, the surface of bacteria became rough and rumply, and the cytomembrane was ruptured after treated by antimicrobial peptide from intestine of grass carp.

结果显示，经过抗菌肽处理后的细菌形态发生变化，菌体严重变形，表面变得粗糙，出现皱褶，胞膜破裂。

The experiment results showed the characteristics of the fermental Toxigenic Pasteurella multocida could meet the standard of producing AR vaccine in the colony morphology, toxin pathogenesis, and other aspects. The serotype of fermental Toxigenic Pasteurella multocida did not change.

实验结果表明，在适宜条件下D型产毒素Pm13-1株培养物符合其菌落、菌体形态特征；糖发酵实验结果符合其生化特性；其产毒素能力符合对制苗菌株产毒素、致病变能力的要求，且未发生血清型的变化。

Ca-alginate immobilized Phanerochaete chrysosporium and Trametes versicolor removed 79.4% and 85% COD from bleaching wastewater at the seventh day, compared to 76% and 81% COD removed by free fungi. Hence, it is demonstrated that the microbial immobilization is highly feasible to treat the bleaching wastewater for discoloration and COD reduction.

而以海藻酸钙固定的白腐菌Phanerochaete chrysosporium 与Trametes versicolor，对制浆废水COD在第七天有79.4%及85%的去除率，高於同时间自由生长的菌体COD去除率76%及81%，因此本研究证明固定化微生物处理制浆废水有很高的可行性。

The recombinant pET-Lip vector was transformed into E.coliBL21 and induced to express by 1mmol/L IPTG at 37℃. An expected 80kDa fusion proteinwas expressed. SDS-PAGE analysis showed the fusion protein located in the supernatant ofbacteria lysate by sonication. The fusion protein was purified by HisTrap~ HP Kit and coulddegrade tributyrin.

该菌株经IPTG诱导可表达分子量约80 kDa的融合蛋白，SDS-PAGE分析表明融合蛋白位于菌体超声裂解后的上清中，用蛋白纯化试剂盒HisTrap~ HP纯化后得到单一的目的蛋白，约80 kDa，且纯化的融合蛋白具有脂酶活性，可以分解三丁酸甘油酯。

The immunofluorescence was developed with the mouse anti-VP4 serum. The results indicated that there was green-yellow fluorescence on the cell surface of pNZ8112-VP4 and pNZ8112-VP4-LTB when induced. The cells uninduced did not display an immunofluorescence reaction and were dyed red by Evans blue.

采用抗VP4的鼠血清进行免疫荧光试验的结果显示，pNZ8112-VP4和pNZ8112-VP4-LTB诱导后的菌体表面能看到黄绿色荧光，而诱导前的菌体则没有荧光反应，且细胞被伊文思蓝染成红色。

The immunofluorescence was developed with the mouse anti-VP4 serum. The results indicated that there was green-yellow fluorescence on the cell surface of pPG-1-VP4 and pPG-1-VP4-LTB when induced. The cells uninduced did not display an immunofluorescence reaction and were dyed red by Evans blue.

通过间接免疫荧光方法鉴定，pPG-1-VP4和pPG-1-VP4-LTB的菌体表面均能看到黄绿色荧光，而未诱导的菌体则没有荧光反应，且细胞被伊文思蓝染成红色。

SDSPAGE analysis revealed that recombinant endostatin was cumulated up to 25% of total soluble protein of E.

经SDS-PAGE分析表明，表达菌株BL-ENDO大量表达了Endostatin蛋白，其表达量占菌体可溶性蛋白的25％。

It showed that the growth of the strain was positive correlated to the flocculating activity at different cultivation time.

通过研究该菌株在不同培养时间的生长情况和发酵液的絮凝活性，从而得出发酵液絮凝活性与菌体生长量呈正相关。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。