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Differential hybridization was used in this experiment to isolate genes expressed in hyphal from specifically.

分别以白色念珠菌菌体和菌丝mRNA为模板合成探针,进行差别杂交筛选。

The purpose of this study is to express VHb gene in Bt, and to improve cell growth and insecticidal crystal proteins production by enhancing the oxygen usage ability of cell.

本研究将透明颤菌血红蛋白基因表达于Bt中,通过提高细胞对氧气的利用能力,达到促进菌体生长和产物合成的目的。

Leucopus and bites humans; principal vector for Lyme disease in eastern United States; northern form was for a time known as Ixodes dammini.

寄生在老鼠身上的扁虱,咬人;美国东部地区莱姆关节炎螺旋菌主要的携菌体;北方的虱子一度被认为是硬蜱。

E. coli cells harboring the recombinant plasmid was cultured with Laria broth medium and induced with isopropyl β-D-thiogalactopyranoside and detected from supernatant and precipitate of culture lysates by using SDS-PAGE.

将重组菌培养并经IPTG诱导后用超声波裂解菌体,取上清和沉淀同时作SDS-PAGE电泳后显示,上清中没有热休克蛋白A的表达,在沉淀中有热休克蛋白A的表达。

Magnesium at concentrations between 3?15 g/L in growth media promoted the growth of mycelia of A.

g/L的Mg浓度对于蜜环菌菌体的生长有促进作用。

To investigate the bioactivity of Nocardia rubra Cell, the mice were used to assay the toxicity, the effects on immune organs, phagocytes of peritoneal macrophage and the antitumor activity by perfusion of NC to the stomach of mice.

摘 要:为了考察红色诺卡氏菌菌体的生物活性,通过一定浓度的NC对小鼠灌胃给药,检测其毒性及对免疫器官、巨噬细胞吞噬功能的影响和对肉瘤S180抑制作用。

Palmitoleic acid is 52.14% of total oil and fat. According to the results of identification, the strain is considered of belong to species Saccharomyces cerevisiae.

该菌株产油脂量为菌体干重的32.06%,棕榈油酸占油脂总量的52.14%,经初步鉴定认为是一株酿酒酵母Saccharomyces cerevisiae。

The characterization of the fermental Toxigenic Pasteurella multocida (Pm 13-1) is studied according to the vaccine standard.

为检验该培养方法培养出的T+Pm是否符合对T+Pm制苗用菌液的生物学特性鉴定标准,分别取样作培养特性检验、菌体形态检验、糖发酵实验、T+Pm毒素产生的检测及荚膜型鉴定。

With the inducing time prolonged, the amount of fusion protein increased, and the highest content amounted to 39. 14% of total protein. Different temperature (15℃, 25℃ and 37℃) could induce expression of fusion protein, and the higher inducing temperature was, the more total fusion protein and soluble fusion protein was produced. Most fusion protein was expressed in the cytoplasm and was soluble, a little of fusion protein was expressed in periplasm or expressed as inclusion bodies, and no fusion protein was found in culture. Furthermore, the results of in vitro enzymatic reaction suggested that the fusion protein could catalyze the methylation of theobromine.

随着诱导时间的延长,表达的融合蛋白产量逐渐增加,表达蛋白总量最高可达到菌体总蛋白的39.14%;在15℃、25℃和37℃三个不同的诱导温度下,均能诱导产生融合蛋白,而且产生的融合蛋白的总量及其可溶性部分所占比例均随诱导温度升高而增加;在菌体的各个部位中,融合蛋白主要在细胞质中以可溶的形式进行表达,有少量在外周质中表达或以包含体形式在细胞质中表达。

Five bacteria strains were isolated from the intestine of Pieris larvae by traditional methods of separation, purification and cultivation.

从自然种群的菜青虫肠道环境中按传统分离、纯化、培养,获得细菌5个菌株,对其菌体形态、染色反应、培养性状、生理生化反应进行了系统研究。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

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不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。