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Methods The selected microorganisms were cultivated at 37℃ in culture media with different concentration of sodium arsenite and counted by microscope, and arsenic content in bacteria was detected by ASA.

采用在培养基中加入不同浓度亚砷酸钠的方法研究各菌株对砷的耐受性,并在显微镜下进行细菌量计数,采用超低温捕获原子吸收分光光度仪测定菌体中砷含量。

An antibiotic derived from cultures of the bacterium Streptomyces lincolnensis, used in the treatment of certain penicillin-resistant infections.

林肯霉素从林肯链酶菌的培根菌体中提取的一种抗菌素,用于治疗某种抗青霉素的感染

With the optimization of the cultivated conditions, the recombinant fusion protein staphylokinase-hirudin was over-expressed in E.coli BL21(DE3), and the final cell density reach 115g wet cell weight per liter, the target protein is about 1.1~1.2g/L accounting for more than 30% of the total protein. The culture process in 5L fermentor was performed on 40L fermentor successfully, and indicated that the process was easy scalable.

通过对培养条件的不断优化,重组葡激酶-水蛭素融合蛋白在大肠杆菌BL21(DE3)里得到了高效表达,菌体密度最终达到115g/L以上,可溶性重组融合蛋白占菌体总蛋白的30%以上,含量约为1.1~1.2g/L.5L发酵罐的发酵工艺参数在40L发酵罐中进行了放大培养,结果表明该工艺能有效的放大,可适用于工业生产。

After cultivating in liquid medium for 5 days, an extra cellularβ-mannanase from Athelia rolfsii strain CBS191.62 was purified 15.1-fold toelectrophoretic homogeneity by ammonium sulfate precipitation, DEAESepharose Fast Flow chromatography, Hydroxylapatite chromatography andrefrigeration crystal.

产β-甘露聚糖酶的Athelia rolfsii菌株CBS 191.62,发酵培养5d,发酵液离心去菌体,上清经硫酸铵沉淀,琼脂糖凝胶层析、羟基磷灰石层析和冷冻干燥结晶等步骤,β-甘露聚糖酶的比活提高了15.1倍,获得凝胶电泳均一的蛋白样品。

The SEM graphs showed that PAO1 only can form scattered cenobium in iron-depletion environment.

PAO-JP2正常情况下形成的被膜结构就很不典型,菌团小且稀疏、散在的细菌多;缺铁时仅可观察到少量单个菌体黏附在硅胶膜表面。

The specific activity of the granules with different diameters and under different temperatures was investgated as well. the results showed that the dominent bacteria change from cocci to bacilli in the granulation, and concerning...

结果发现污泥颗粒化过程中菌体的变化趋势是以球菌占优势,逐步过渡到杆菌占优势,并且从比活性和沉降性能考虑,有必要对颗粒污泥最佳直径的控制和确定问题进行研究。

The construction of recombinant UNPase provided a basis for the research on biocatalysed synthesis of adenosine. In comparison with uridine, inosine was the better donar of pentose-1-phosphorate. In the reaction mixture composed of 30 mmolL^(-1)inosine, 10 mmolL^(-1) adenine, and 30 mmolL^(-1) pH 7.5 phosphate buffer and 0.5% wet cells gave a high yield of 85.57% at 60℃ in 3h.

在此基础上研究了不同诱导条件对蛋白表达量的影响分别以肌苷和尿苷为核糖供体研究重组菌转化腺苷的条件,发现肌苷最适合作为核糖供体,在30mmolL^(-1)pH7.5的Na2HPO4-KH2PO4缓冲液中,以30mmolL肌苷和10mmolL^(-1)腺漂呤作底物,加入0.5%基因工程湿菌体60℃反应3h,腺苷的转化率为85.57%。

It is of great significance to grow the strain into the appropriate pellet form for the industrial fermentation of filamentous fungi.

发酵过程中控制菌体为适宜的菌球形态对于丝状真菌发酵的工业化放大具有重要的研究意义。

Six thermophilic bacteria were isolated and purified from a hot spring in Chang-bai mountains,One of those morphology has been studied detailedly,it's growth course has been observed,we find it's morphology is very peculiar,at different growth phase,it's morphology changed very evidence,in the logarithmic growth phase,the cell is rod, majority cells are single existence,minority are geminous or a long discontinuous chain,in the middle growth phase,rod and globe are concomitant,in the late stationary growth phase, the cells change to globe.

从长白山中一个温泉里面分离,纯化出来六株高温菌,我们对于其中一株CW2的个体形态做了详细的研究,对它的生长过程连续监测,发现它的个体形态比较特别,在不同的生长阶段,它的个体形态变化非常明显。菌体在对数生长期初期是杆状,大多数单生,少数对生或者形成间断的长链,在中期就是球形和杆形共存,在对数生长期的末期基本上是球形。

The higher the inoculation concentration of the strain is during seed germination, the more quantity the strain in host plant tissues. When strain B504-1121 was inoculated on lower petioles, it could transfer into upper tissues, however it only habitate in leaves if it was inoculated on leaves.

在下部叶片叶柄处接种的菌体可向上移动,通过茎进入上部叶片;在叶部接种的菌,则只在接种叶内定殖,不再向其他部位转移。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。