菌体

After running for 36 h,the strains in the fermenter were diluted to the range of 50～200 g/L for the succedent continuance production.

生产36 h后对发酵系统中菌体进行稀释，使菌体量保持在50～200 g/L之间可持续进行生产。

When the strain grew in the medium containing glucose(40 g/L) as carbon source with tomato juice(3.6 mL/L), gingili oil(1.6 mL/L), Na2CO3(6 g/L)and phosphate buffer, at 28 ℃ with illumination for 120 h, its cell biomass, and carotenoid yield and content could reach 65.57 mg wet cell per mL medium, 9.896 μg per mL medium and 150.9 μg per gram wet cell, respectively.

菌株在以葡萄糖为碳源，添加番茄汁（3.6 mL /L）、芝麻油（1.6 mL/L）、Na2CO3（6 g/L）和磷酸缓冲盐的液体培养基中28 ℃振荡培养5 d，每mL培养液细胞生物量湿重达到65.57 mg，类胡萝卜素产量达到9.896 μg，菌体每g湿重细胞胡萝卜素含量为150.9 μg。

The expression of spinach glycolate oxidase in pichia pastoris appeared to be induced by methanol, the molecular weight of glycolate oxidase was about 39.8kDa by SDS-PAGE and consisted with the data of literature.

甲醇诱导重组菌表达，并对诱导的条件进行优化，确定甲醇最适剂量为0.5%，最佳表达时间为72hr，最适温度为28℃，最佳pH为5.5，最佳表达菌体密度A600为0.8。

The morphological change of Escherichia coli were observed by transmission electron microscope, and the antibacterial mechanism of Fe/Ce codoped nano-TiO2 hydrosol was discussed.

采用透射电镜对杀菌过程中的大肠杆菌菌体形态进行观测，探讨了Fe/Ce共掺杂纳米TiO2水溶胶的抗菌机理。

As a result penicillium of Ma Erni humble is double photograph bacterium, mould bacterium colony can discover the broom shape mycelial of diagnostic sex,; of pigment of rose of water-solubility of the generation after sanded Paul fosters 3 days is medullary inside and outside of cell of blood of the week outside mixing all can discover spore;PAS coloring sees bacterium body show circle, elliptic or sausage shape, size is differ, it is 2~8 μ M about, color of afterbirth wall incarnadine and clear and successive, it is thus clear that inside the cell of sausage shape one apparent horizontal stroke is lain between, afterbirth is qualitative not easy and chromatic.

结果马尔尼菲青霉菌为双相菌，霉菌型菌落可发现特征性的扫帚状菌丝，沙保罗培养3天后产生水溶性玫瑰色素；骨髓和外周血细胞内外均可发现孢子；PAS染色可见菌体呈圆形、椭圆形或腊肠状，大小不一，约为2~8μm，胞壁染红色且清楚连续，在腊肠状的细胞内可见一明显的横隔，胞质不易着色。

The effects of glucose and inorganic phosphate on mycelium growth and spinosad production with Saccharopolyspora spinosa were studied.

考察了葡萄糖及无机磷酸盐对刺糖多孢菌菌体生长及多杀菌素产量的影响。

Coli BL21（DE3） which expressed Ag473-3His do not cross reaction with human neuroblast, such finding make this lipoprotein a potential vaccine candidate.

coli BL21（DE3）全菌菌体免疫产生的抗体并不会和人类神经原母细胞产生交叉反应，因此成为疫苗的首选。

We used Aeromonas hydrophila which we separated from the diseased Paralichthys olivaceus to made a inactivated whole cell bacterin .We injected this vaccine in the mice and the Paralichthys olivaceus .Both the mice and the Paralichthys olivaceus gained a good protection of 80% and 70%.The fish with dipping immersion gained a good protection of 85%,and the incidence of disease was 6%.We detected the antibody level in the antiserum by agglutination reaction and indirect ELISA which seemed to be immunogenic crucially.We anatomized the mice then we found that all the purtenance of the immunzied mice are healty.All of this proved that the vaccine is effective.

本实验将所分离到的嗜水气单胞菌用终浓度为0.2%~0.5%的甲醛对菌体进行灭活，制备了灭活疫苗，并优化灭活疫苗的灭活条件；将制备的灭活疫苗分别注射小鼠和牙鲆，均取得了较为理想的效果，其中小鼠的免疫保护率达到80%，牙鲆的免疫保护率达到70%；采用渗透压差法大规模浸泡免疫牙鲆也取得了很好的效果，浸泡免疫过的牙鲆发病率仅为6%，攻毒后免疫保护率为85%；通过应用间接ELISA方法检测受免动物的抗血清，并将牙鲆的抗血清注射新西兰白兔，从而获得兔抗鱼血清，更便于检测，结果都表明动物体内产生了较高的抗体水平；剖检攻毒后的动物，发现免疫过的动物内脏健康无异样，表明该疫苗安全有效，从而为防治牙鲆腹水症开辟了一条新途径，也为进一步制备其基因工程疫苗奠定了基础。

Furthermore, the analysis of X- ray diffraction diagram indicated that cadmium ion was transformed into cadmium sulfides by H strain. Also, we found that the removal for cadmium was dependent on the growth stationary phase of H stain through determining the removal rate for cadmium, the growing curve of cell and the activity of cysteine desulfhydrase.

通过X射线衍射光谱分析，H菌株对镉离子的转化产物为硫化镉，通过测定镉的去除与细菌生长曲线及半胱氨酸脱巯基酶的活性，表明镉的去除与菌体的生长稳定期相关。

The central composite rotatable design and response surface analysis were then used to determine the optimal levels of the main factors.

筛选出CaCl2与豆粕水解液为STX2的生长强化因子，且培养基的初始pH值也是影响该菌体生长的关键因子，而其他物质对STX2增菌量的影响不显著。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。