菌体

In conclusion, the lag phase of Streptococcus thermophilus was shorten in the eutrophy culture medium, and the time achieving the culminated point of logarithmic phase was shorted also, at the same time, the stationary phase lengthened. After secondary culture, Streptococcus thermophilus reached directly the log phase hardly through the lag phase, and then quickly reached the decline phase. There were more relations of the metabolic regalities of the three kinds of nutritive substances with the super-concentrated incubation process of Streptococcus thermophilus: the more the number of the bacteria, the quicker the metaboling, while the fewer, the slower the metabolism. Moreover, there apparently existed morphologic changes in this course, and there maybe existed the apoptosis, which correlated with bacteria propagation and acid producing. With the culture time postponed, the nutritive substances in the culture medium lacked also, and the morphologic change and apoptosis appeared more obviously.

结果表明，嗜热链球菌在营养丰富的培养基中的生长延滞期缩短，到达对数生长顶点的时间变短，稳定期的时间延长，当经过二次培养后，嗜热链球菌几乎不经过延滞期直接到达对数期，平稳期持续的时间较短，很快到达衰亡期；嗜热链球菌在超浓缩培养过程中三大营养物质的代谢规律也同它的培养过程有很大的关系，菌数多，其代谢旺盛，菌数降低其代谢速度减慢；嗜热链球菌在超浓缩培养过程中存在着明显的菌体形态变化，并可能存在着细胞凋亡，与菌体增殖和产酸规律密切相关，随着培养时间延长，培养基中营养物质变得贫乏，菌体形态变化和细胞凋亡的情况愈加明显。

The results showed that the Saccharopolyspora spinosa SP06081 protoplast yield was the highest under these conditions: the collected mycelium from SP06081 grown in Tryptic Soy Broth medium with 0.2% glycin for 48 h was treated by 0.1 mg/mL lysozyme at 28oC for 20 min, then plated on the R2YE medium with sucrose as osmotic stabilizer, the number of regeneration protoplast was up to 108/mL. The protoplast-regenerated strains exhibited changes in morphology and antibiotic production, 29.3% protoplast-regenerated strains was characterized by loose mycelium and abundant broken branches as did their parent. Among them, 58.2% strains presented the trend to positive variation in spinosad yield, with the highest spinosad yield of up to 582.0 mg/L, 85.6% higher than that of their parent.

结果表明：菌体在添加0.2%的甘氨酸的TSB培养基中培养48 h收集， 0.1 mg/mL 溶菌酶， 28oC作用20 min制备原生质体，将原生质体涂布于以蔗糖为渗透压稳定剂的R2YE培养基中，原生质体再生数目最多，达108个/mL以上；原生质体再生菌株在形态和抗生素产量上产生分化， 29.3%的再生菌株形态上保持与亲本菌株一致，具有菌丝松散，断裂分枝多的特点，其中53.2%的再生菌株多杀菌素产量变异向正方向移动，最高产量达到582.0 mg/L，比亲本菌株提高85.6%。

The citric acid-producing strain Aspergillus niger 2363-2, was isolated and used in our research. The fermentation conditions were optimized as follows: sucrose as carbon source 8. 0-14. 0%, ammonium chloride as nitrogen source 3. 0-4. 0g/l, initial pH 2. 0, and cultural temperature 30℃. The influential factors of formation of spores were also studied, and a series of measures were taken to restrain the spore formation on the basis of our experimental results. With the consideration of the cultural environment and microbial cell itself, the factors affecting the duration of citric acid-producing activity of microbial cells were examined, and it is found that undissociated citric acid and the ageing of microbial cells are the main factors. The experimental results also show that partial replacement of fermentation broth can lengthen the duration of citric acid -producing acitivity of microbial cells.

筛选出了用于试验研究的柠檬酸发酵用菌Aspergillus niger 2363-2，确定了其最适发酵条件—碳源选用蔗糖，浓度8.0~14.0%，氮源为NH〓Cl，浓度3.0~4.0g/l，发酵初始pH值为2.0，培养温度30℃；讨论了分生孢子产生的影响因素，并通过试验提出了抑制分生孢子产生的措施；从分析微生物所处的环境和微生物本身两个方面出发，探讨了影响微生物菌体产酸活性持久性的因素，认为分子态柠檬酸和菌体本身的老化是影响微生物产酸活性持久性的两个主要因素，并提出采用部分置换发酵液发酵能延长微生物菌体的产酸活性。

The study of the effect of Glycerol on survival of the E.coli shew Glycerol could protect E.coli and improve dry, vacuum and implantational survival rate during ion beam implanting the E.coli. The whole survival rate of protected the E.coli implanted by 30keV N+ was 530-10000 times higher than unprotect ones. Glycerol changed bacteria outer color and survival rate curve. At the same doses, the color of the E.coli protected by five and one percent Glycerol was white or yellow, lighter than unprotected. In addition, the survival rate curve of the E.coli with Glycerol located above one without Glycerol, and the peak of which was higher and delayed at dose.

在研究甘油对低能离子注入E.coli存活的影响时，发现甘油保护可以提高E.coli的干燥存活率，真空存活率以及注入存活率，使得整个30keY氮离子注入后E.coli的最终存活率提高530-10000倍；甘油保护引起离子注入后的菌体颜色发生变化，在相同的剂量下，未用甘油保护的菌体颜色最深，1％甘油保护菌体颜色次之，5％甘油保护菌体颜色最浅；另外，甘油可以引起离子注入E.coli的"马鞍型"存活率曲线发生变化，甘油保护的E.coli存活曲线大致位于未用甘油保护的存活曲线的上面。

HP3 identified as Zoogloea sp. is an effectively strain. Under the optimal degradation condition and ABAS concentration of 200～1200mg/L, the ABAS degradation followed negative exponential model. With the ABAS concentration increasing, the ABAS degradation was greatly regressed. Temperature was the most important factor influencing ABAS degradation. The functions of specific growth rate and temperature, degradation rate of ABAS and temperature were constructed respectively.

菌株HP3是一高效降解溴胺酸的菌株，经鉴定为动胶菌属；在最佳降解条件下，溴胺酸浓度在200～1200mg/L时，其降解遵从负指数方程，溴胺酸浓度大于1200mg/L时，降解受到显著的抑制；温度是影响菌体生长及降解溴胺酸的最显著因素，建立了菌体比生长速率-温度和溴胺酸降解率-温度曲线方程。

Results We discovered that under starvation conditions, the long, flexible rod cell of Cytophaga hutchinsonii would bend and turn into circular cell. The circular cell failed to produce carboxymethyl cellulase. Some of the circular cells might further wind around and turn into tiny spherical cells.

发现在饥饿状态下，长杆状菌体开始逐渐弯曲，菌体两端靠近成环形，环形菌体又进一步盘绕收缩成微小球形体，微小球形体在一定条件下能像生孢噬纤维菌的小孢囊一样萌发形成长杆状菌。

The study showed that the biosorption process could be described well by pseudo-second-order kinetic model and Freundlich isotherm model.Higher cell concentration was favorable to the biosorption.Temperature's effect on the biosorption was not obvious.The oxygenous and nitrogenous functional groups on the cell wall played a very important role in the process of biosorption.Microdeposits were formed by int...

结果表明，菌体对银的吸附量可达91.75 mg/g，吸附过程符合Pseudo-second Order吸附动力学模型，相关系数高达0.999 9；吸附热力学很好地符合Freundlich吸附等温模型，相关系数为0.99；考察菌体浓度和温度对吸附的影响发现，菌体浓度增加有利于对银离子的吸附，温度对吸附影响较小；FTIR、SEM及EDAX实验结果表明吸附存在2种吸附机理，一为菌体表面一些含氮氧的基团对Ag+的络合作用，二为菌体分泌的胞外多糖等物质对Ag+的微沉淀成晶作用。

During the stationary phase of fermentation, cell productivity was slightly reduced. MgSO_4 and tryptone have the positive effect on the product synthesis and cell growth respectively. Based on these conclusions, MgSO_4 and tryptone were added to the feed medium to enhance the production of polysialic acid. Variable feed rate policies were applied to the fed-batch fermentation process and the final biomass and polysialic acid production were both increased by 40 percent.

通过摇瓶实验研究了大肠杆菌发酵过程中菌体生长和产物合成的影响因素，结果表明：产物合成与菌体生长是相耦联的过程，菌体生长受到菌体代谢产物的抑制作用，聚唾液酸的合成不受代谢产物的抑制作用；发酵进入稳定期后菌体的生长和生产能力有所降低；硫酸镁和胰蛋白胨分别对产物合成和菌体生长有一定的促进作用。

Here, we demonstrated in this work that CsrA performs a negative regulator of the cel expression. cel, encoding lysis protein (LysE7), is located downstream of the colicin E7 structural gene in the ColE7 operon. Lysis protein was essential for colicin release and causes a decline in culture turbidity as well as lethality of the host cell when overproduced. Western blotting analysis of the level of LysE7 in the wild-type and csrA mutant strains was examined.

在本论文中，我们进一步发现到CsrA对於cel 基因的表现具有抑制的现象。cel 基因位在质体ColE7-K317上的E7大肠杆菌操纵子，可以制造出溶菌蛋白质(lysis protein， LysE7)，协助大肠杆菌素及免疫蛋白质的复合体运送至菌体外；但当菌体产生过多的溶菌蛋白质，则会导致宿主细胞的死亡，所以溶菌蛋白质的表现必须受到严密的调控，避免菌体产生过量，而CsrA则是目前第一个被发现到能调控溶菌蛋白质表现的因子。

Times higher than that of initial strain. Polysaccharides, such as barley flour, dextrin and soluble starch, were better carbon sources than monosaccharides and disaccharides, such as glucose and maltose, for cell growth of B subtilis ZJF-1A5 and β-glucanase production. Yeast extract was the best nitrogen source, the following was soybean flour.

糊精、大麦粉等多糖有利于B.subtilisZJF-1A5 β-葡聚糖酶的产生，葡萄糖、蔗糖等易利用性碳源不利于菌体生物量的积累和β-葡聚糖酶的产生，β-葡聚糖酶受分解代谢产物阻遏调节。B.subtilis ZJF-1A5 β-1，3-1，4-葡聚糖酶的产生与菌体生物量和菌体生长状态密切相关，提高发酵产酶水平的关键是提高菌体生物量和解除葡萄糖效应。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。