菊粉酶

Inulin utilizing strain was screened from the soil which Jerusalem Artichoke was planted as well rotten chicory.

本文从菊芋生长的土壤、腐烂的菊芋及实验室保存的菌种出发，筛选到产菊粉酶较高的菌株。

The main results are shown as follows:1.Screening freezing tolerant cultivars and lines of chrysanthemumTwenty seven lines of chrysanthemum grown outdoors were screened using \'injury rate\' and \'injury index\'.There were 7 cultivars and lines,which were relatively high tolerant to freezing treatment,i.e.,\'Jinbuhuan\',\'Xuetao\',\'Fuli\',\'Donglin 4\',\'Donglin 6\',\'Donglin 9\', and \'Donglin 13\'.There were 3 cultivars and lines,which were less tolerant,i.e.,\'Fenmeigui\', \'Donglin 1\',and \'Donglin 2\';The determination of electrolyte leakage,and contents of free proline,MDA,CAT,fructose,glucose and soluble sugars on 7 lines of chrysanthemums at different stages freezing showed that \'Xuetao\' and \'Donglin 4\' were more tolerant than others, and that especially \'Donglin 4\' was able to regulate its osmotic pathway to alleviate the \'injury degree\' caused by freezing conditions.2.Protein expression profile of chrysanthemum under low temperature stressTwo-dimensional liquid chromatography was applied to fractionate and analyze total protein taken from \'Donglin 4\' leaves grown at low temperature and at normal condition. Chromatography peaks of fraction were transformed to gel graph by ProteoVue software.

主要研究结果如下：1、露地菊抗冻品种和品系的筛选利用受伤率和伤害指数两项指标对27个露地菊品系进行耐低温筛选，筛选出'金不换\'、'雪涛\'、'富丽\'、'东林4号\'、'东林6号\'、'东林9号\'、'东林13号\'等7个耐低温品系，同时发现'粉玫瑰\'、'东林1号\'、'东林2号\'三个品系的耐低温能力最差；通过对不同自然低温处理时期，7个品系的电导率、丙二醛含量、脯氨酸含量、过氧化氢酶、果糖、葡萄糖和可溶性糖等指标的测定发现，'雪涛\'和'东林4号\'的耐低温能力要强于其它品系，尤其是'东林4号\'对低温的调节能力较强，在低温胁迫下，能够调节渗透途径减轻低温的伤害。2、低温胁迫下，露地菊蛋白质表达谱分析利用二维液相色谱技术，分离了'东林4号\'露地菊品系的低温处理和对照样品的总蛋白，通过ProteoVue软件分析发现：处理样品与对照相比共有蛋白差异点36个，其中显著增强表达的蛋白差异点有26个。

Three mold stains, C122803、D081506 and D081513, which had higher ability of producing inulase were obtained by using transparent circle method as initial screening and rocker method as rescreening.

通过透明圈法初筛及摇瓶复筛，获得产菊粉酶能力较高的霉菌菌株3株，为C122803、D081506和D081513，这3株菌株的酶活分别达到：C122803：1.411 U/ml；D081506 ：1.895U/ml；D081513 ：1.792U/ml。

Inulinases is an important enzymes which involving in the production of fructose and fructooligosaccharides from inulin hydrolyzation.

菊粉酶是一类能水解菊粉生产果糖和果聚糖的酶。

Inulinase can hydrolyze inulin into fructoses and fructooligosaccharides which have great applied value and broad prospects in food industry.

许多菊科植物中含有丰富的菊粉，利用菊粉酶的作用可将菊粉转化为果糖、低聚果糖，在食品工业中具有重要应用价值和良好应用前景。

In this review, the recently advances in enzymatic properties and molecular biology of inulinase were summarized.

本文对菊粉酶的酶学性质、基因和基因工程的最新研究成果进行了综述。

The synergism ratios of piperonyl butoxide to alpha-cypermethrin in these three strains were similar(about 20-fold), which suggested metabolic detoxification probably confers about 20-fold inherent resistance in the B-type whitefly, as PBO is a mixed function oxygenase and esterase inhibitor and glutathione S-transferase is not affinity to resistance in B. tabaci. The synergism ratio of triphenyl phosphate to alpha-cypermethrin in the NJ strain was 12-fold, which implied the synergism of PBO in the B-type whitefly is mainly due to inhibiting esterase activity.

增效试验表明PBO对这三个品系的增效作用均为20倍左右，因为在烟粉虱中PBO即是多功能氧化酶抑制剂，也是酯酶抑制剂，而谷胱甘肽S-转移酶与抗性无关，因此推断代谢酶在B型烟粉虱对高效氯氰菊酯的抗性发展中起到的相对作用可能为20倍左右，而且这种抗性是B型烟粉虱天生的；TPP对NJ品系的增效作用为12倍，说明PBO增效作用主要是由于抑制酯酶而引起的，即代谢抗性大部分由酯酶所贡献。

The results are published in the Food and Chemical Toxicology , and used Beneo-Orafti's Orafti P95 (formerly Raftilose 95) derived from chicory root inulin by enzymatic hydrolysis.

这个结果发表在食品与化学毒理学期刊上，实验是通过一种主要由菊苣根部菊粉部分酶水解产生的低聚果糖 Beneo-Orafti's Orafti P95 （正式叫做果寡糖95）。

At present, there are many reports regarding inulinase production from various fungus, yeast and bacteria strains.

目前，已有许多关于各种霉菌、酵母和细菌生产菊粉酶的报道。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。