获得物

Two pairs of primers were designed based on the sequence of ovis aries preprochymosin cDNA reported in GenBank.The preprochymosin cDNA was obtained from toltal RNA isolated from the abomasum of 5-day-old Xinong Saanen Goat by RT-PCR method and then cloned successfully in E.coli.

参照GenBank登录的绵羊凝乳酶原前体cDNA序列设计引物，以新生5d的西农萨能羊皱胃组织总RNA为模版，通过RT-PCR方法获得凝乳酶原前体cDNA，克隆测序后进行序列比对。

ThemostpotentofthesetoxinsThe coding sequence of abrin a A chain gene was obtained by RT PCR and cloned into the expression vector pET28b.

成熟的ABRaA在大肠杆菌中得到高效表达，可溶性重组蛋白质的获得率达 4mg/L培养物，而且具有较好的纯度。

Then, two primers were designed from it, the full length cDNA of malate dehydrogenase gene in Chinese cabbage was gained through RT-PCR amplifying and sequencing and entered GenBank as accession number FJ208590, which is 1 209 bp length and coded 403 amino acids.

然后设计引物，经RT-PCR扩增、测序，获得了大白菜苹果酸脱氢酶基因的cDNA全长序列，并已将其登录到GenBank，登录号为：FJ208590，该cDNA全长1 209 bp，编码403个氨基酸。

The optimized structures and energies of the reactants, intermediates, transition states and products of two reaction channels (corresponding to syn-acraldehyde and anti-acraldehyde) in gas or in methanol solvent were obtained. The potential energy profiles reveal the processes of the trimethylamine-catalyzed Baylis-Hillman reaction between acraldehyde and formaldehyde in gas or in methanol solvent at the microscope level.

通过研究，获得了三种情况下两种反应通道(分别对应于顺式-和反式-丙烯醛)中涉及的反应物、产物、中间体、过渡态的优化结构及其能量，并由此得到了反应的势能面图，在电子微观层次上揭示了三甲胺催化的丙烯醛和甲醛的Baylis-Hillman反应的微观过程。

In this work, a series of layered silver-long chain alkylamine complex has been synthesized in the alcohol/water system for the first time, and such compound possesses the special sponge-like macroporous structure.

在本工作中，系列银-不同碳链长度的有机胺的配合物首次在醇-水体系中被合成获得，且该化合物具有特征性的海绵形貌中空结构。

Through the in-situ reduction with KBH_4, a series of the silver(0)-alkylamine complexes has been obtained, the special structure and morphology of such kind of complexes will find wide application in the construction of catalysis, photonic or electronic materials.

通过硼氢化钠还原后，获得了系列以金属银为层板的海面状复合物，具有特殊结构的该类化合物可能在催化，光电材料等领域具有广泛的用途。

The new methods allow a wide range of poly analogs to be obtained in few steps, as the allylic precursors are readily available.

新方法允许范围广泛的聚类似物，以获得在几个步骤，作为烯前体是现成的。

The author creates the AutomaticallyDiscriminational Model for the Classification of Sandstones System, thatis to say, people can complete the vastly sample s classification of sandstonesaccurately and swiftly, meantime, diminish the possibility of mortal mistake. Based on the study of the basic sandstone component before embedding, the typeof authigenic mineral, and the forming mechanism of porosity in Upper Paleozoicreservoirs in Ordos basin, the author selects the reasonable experiment objects forWater-Rock Interaction modeling study, designs the relatively reactant and resultantduring the diagenesis period, builds the solid-liquid thermodynamics model with theprinciple of thermodynamic phase equilibrium for kinds of primary minerals (potashfeldspar, albite, anorthite, and pyroxene, hornblende) which dissolve in acidic media,and forms the corresponding thermodynamics test database.

在对鄂尔多斯盆地上古生界储层砂岩埋藏前组成、自生矿物类型和孔隙构成研究的基础之上，确定合理的沉积盆地水—岩相互作用模拟研究的实验对象，设计了埋藏成岩过程有关反应的反应物和可能的生成物，并通过热力学相平衡原理，建立了碎屑岩在埋藏成岩过程中各种骨架颗粒(钾长石、钠长石、钙长石以及铁镁暗色矿物中的辉石、角闪石等)在酸性介质条件下发生溶解的固体—流体相化学反应热力学计算模型，并获得了相应的热力学数据库，在此基础上对储层砂岩中主要造岩矿物(钾长石、钠长石、钙长石以及铁镁暗色矿物中的辉石、角闪石等)在埋藏成岩过程的酸性介质条件下发生溶解的热力学习性进行了论述。

Value your possessions based on their approximate replacement value.

确定能够获得赔偿的物品价格应与被保险物的价格相近。

The retorting process of coals using hot char as solid heat carrier lines up every single process which is needed for the production of CMS.

以灵武煤半焦GLW-72为原料，粉碎至粒度＜160目，混入40wt%的亚硫酸盐纸浆废液成型，将成型物在N〓气保护下，以10℃/min的升温速率加热到900℃碳化45min，冷却到室温所得碳化产品用于变压吸附空分，可获得含N〓96.3vol%的富N〓气体；将上述产品进一步作碳沉积处理，可获得空分性能达98.4vol%的炭分子筛，该结果与在同一空分装置上测得的国外炭分子筛的空分性能（98.23vol%）相当。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。