获得物

Total 122 entophytic bacterial strains were categorized preliminarily to 35 groups based on colony morphology. 16S rDNAs were amplified with universal primers of bacteria, all the strains could produce a single band about 1500bp. The PCR products were digested with restriction endoenzyme HaeⅢand Msp Ⅰrespectively, and the different patterns were obtained for each enzyme, by combining all the restriction patterns for the two enzymes, the 122 strains could be grouped into 39 16S rDNA genotypes. The results indicated that endophytic bacteria from oilseed rape were genetically diversity.

分离获得的122株内生细菌，根据菌落形态可以划分为35类；利用细菌通用引物对16S核糖体DNA进行扩增，获得约1.5 kb片段，分别用内切酶HaeⅢ和MspⅠ对扩增产物进行限制性酶切，产生不同的酶切图谱；根据酶切图谱聚类分析结果，所有供试菌株被归为39类，这一结果从遗传上显示油菜内生细菌类群的多样性。

In various steps of the experimental flow,biological component,clay minerals and feldspar etc.

在流程中的不同阶段可有效去掉全样中的生物组分、粘土矿物和长石等，取得海洋沉积物全样的总陆源矿物、石英+长石、石英的相对和绝对含量，通过计算可获得各种组分的通量，通过粒度分析可获得粒度数据。

The results indicate that CdS nanorods synthesized by soft template method are below 100 nm in diameter and above 3 nm in length, and low irradiative dosage and concentration of reactants can accelerate the formation of CdS nanorods with higher length-to-diameter factor about 30∶1 range.

研究表明，该方法在Cd2+浓度为0.015 mol·L-1、辐照剂量为15～20 kGy时，可获得直径在100 nm以下，长度在3 μm以上的CdS纳米棒；并且较低的辐照剂量和反应物浓度有利于获得较大长径比的CdS纳米棒。

Analysis of the obtained genome amplification showed that DNA after PCR presented obvious polymorphism, and the results were the same using the same primer to amplify the larva and imago of the same species. It indicates that RAPD technique can be applied to the identification of dry samples and dipped larva sample of longicorn beetles as well as the research of phylogeny. These three methods for extracting genome DNA from different species of longicorn beetles at different preserved time are feasible.

对所获得的基因组扩增分析表明，经过PCR之后的DNA能呈现明显的多态性，同种天牛用同一引物扩增幼虫和成虫所获得的结果相同，可以认为，RAPD技术可以应用于天牛成虫干标本及幼虫浸渍标本的鉴定及系统发育研究中，也证明了这3种方法用于提取不同保存时间、不同种天牛基因组的DNA是切实可行的。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

METHODS: Three patients who reported metamorphopsia but showed no changes in the retina in conventional fundus photographs were examined. High-resolution retinal images were obtained with the AO fundus camera and by FD OCT. The images were compared with the findings obtained by standard clinical tests, including Amsler charts and fluorescein angiography.

对报告有视物变形但经传统眼底照相未发现视网膜变化的3例患者进行检查，使用自适应光学眼底照相和傅立叶域光学相干成像获得高频视网膜图像，将所获得的图像和标准临床检查结果对比，这些标准临床检查包括Amsler方格和荧光血管造影。

Taking Eremothecium ashbyii (abbreviated as Eaw95. 1) as the original strain, after Υ-ray mutation, tubercidin and 8--azaguaniae (8--AG) resistant tests, a strain labeled as T〓 capable of overproducing riboflavin was obtained and its genetic stability was analyzed. Compared with Eaw95. 1, the riboflavin productivity was improved about 40% by flask--shaking tests.

以Eremothecium ashbyii为出发菌（间称Eaw95.1），通过Υ-射线诱变，杀结核菌素和8-氮杂鸟嘌呤等结构类似物的的抗性筛选，获得了一株核黄素高产菌T〓，并对菌株的遗传稳定性进行了研究，获得了较好的效果，与出发菌株相比，核黄素的摇瓶发酵产量提高了近40％。

The wine extract of fall-winter grape is significantly higher than that of summer fruit. Local grape wine should be able to reach a better quality level by producing fall-winter fruits instead of summer ones.

另秋冬果制酒成品的抽取物显著较夏果成品高，因此本省葡萄酒一向缺乏抽取物的问题，似可经由秋冬果的生产而获得改善。

His story begins with Sumerian farmers who realised, some time in the third millennium BC, that the surpluses of grain generated within Mesopotamia's fertile crescent could be used as barter for things they did not have.

他的故事开篇描述的是大约在公元前3000年间，苏美农民意识到在美索不达米亚平原新月沃地上产出粮食的剩余部分可以用来进行物物交换，以获得自己没有的东西。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。