获得物

In summary, this research was laid a better foundation to further study of the effects of the second ligands on the electroluminescence properties of europium complexes, and achieved OELD with excellent EL properties.

本研究为下一步开展第二配体的结构对铕配合物的电致发光性能的影响，获得性能优良的铕配合物电致发光器件打下了良好的基础。

Phylogenetic analysis revealed that there exists at least 2 genetical distinct hantaviruses lineages.

结果 选用50个随机引物进行扩增，有5个引物获得清晰、多态性高的RAPD谱带。

In our study,two important lepidopteron pests,beet armyworm and cotton bollworm were surveyed for their endogenous piggyBac.As a result,endogenous piggyBac elements were found in these two insects,and one of the piggyBac elements isolated from the genome of cotton bollworm was intact and thought to be potentially active.1 Clonging and sequence analysis of piggyBac from beet armywormUsing PCR technique,with degenerate primers,a DNA fragment of piggyBac-like element was cloned from the genome of beet armyworm,spodoptera exigua hubner.The DNA fragment was 456bp in length,and the deduced amino acid sequence shares 50%- 78% similarity with other piggyBac elements from insects.But one stop code was found in this DNA fragment.

由于转座子很难用常规的RT-PCR和RACE技术进行克隆鉴定，故本研究在兼并引物PCR的基础上，利用inverse PCR和vectorret PCR技术，不仅调查了鳞翅目两种重要的农业害虫甜菜夜蛾和棉铃虫的內源piggyBac存在情况，同时克隆到了內源性的piggyBac转座子，并且从棉铃虫中获得了一个结构完整、具有潜在活性的piggyBac转座子HaPLE1.1甜菜夜蛾piggyBac转座子基因的克隆与序列分析采用PCR技术，利用兼并引物，从甜菜夜蛾基因组中克隆出一个內源性piggyBac类似因子的DNA片段，并命名为SePLE。

A substrate concentration of 0.277 kg/L, enzyme/ substrate level of 1408 U/g, temperature of 39.2 ℃, and a hydrolysis time of 4.86 hour were found to be the optimum conditions for high antibacterial activity. Under this condition, the bacteria Escherichia coli, Micrococcus luteus and Bacillus subtilis in the hydrolysates had the inhibitory rate of 71.40%, 56.88%, and 64.54%, respectively.

经Design Expert 7.1.2软件优化，得出胃蛋白酶酶解鲶鱼骨蛋白的最优条件为：底物浓度0.277kg/L，加酶量1408 U/g，水解温度39.2℃，水解时间4.86 h，在此条件下获得的水解物，对大肠杆菌、藤黄微球菌及枯草芽孢杆菌的抑菌率分别为71.40%、56.88%和64.54%。

A pair of primers for amplification of σC gene was designed and synthesized according to the published Muscovy duck reovirus S4 gene sequence. The specific RT-PCR product was success-fully obtained and cloned from the Muscovy duck reovirus ZJ99 strain.

参考已发表的番鸭呼肠孤病毒的S4序列设计了1对扩增σC蛋白基因的引物，对mDRV ZJ99株的RNA抽提物进行RT-PCR扩增，获得了特异性的扩增片段。

They thought the substance was the outwardness and had nothing business of "Xin", which resulted in the aftermath that "Xin" couldn\'t obtain the effective explain and took "Xin" as same as substance.

他们认为，物是与心无关的客观的存在，这必然导致最终"心"不能获得有效的解释，而把心也归结为与物等同的存在。

Methods To deter mine the composition and activation of cerebroproptein hydrolysate by aminoacid analyzer,HPLC,SDS-PAG and succinate denydrogenase,to determine the therapeutic e ffectiveness of cerebrprotein hydrolysate by ordinary pressure oxytolerant exper iment and paramnesia experiment.

用氨基酸分析仪、高效液相色谱仪、SDS凝胶电泳和琥珀酸脱氢酶法测定脑蛋白水解物的组成与活性。用常压耐缺氧实验和记忆获得障碍法测定脑蛋白水解物的疗效。

Mn-complexes in which Mn atom ligand with the N atom within ligand can stimulate the recovery of electron transfer and oxygen evolution. The trinuclear Mn-complex is extremely sensitive to the addition of CaCl2. It is suggested that there is an interaction between Ca2 and carboxyl within the trinuclear Mn-complex during photoactivation and this interaction benefits the ligation of Mn atom to the apo-WOC and form an active WOC. Binuclear MnMn complex shows slightly higher efficiency than binuclear MnMn complex in restoration of O2 evolution activity. It is suggested from our results that recovery of electron transport and O2 evolution with synthetic Mn-complexes is affected by different factors. Cl- can stimulate the reconstitution of WOC at the concentration of over 100mM;the maximal recovery of O2 evolution activity requires the presence of CaCl2 and 33 kDa protein polypeptide together. Bicarbonate can stimulate the reconstitution of WOC.

锰配合物中锰原子与配体中的氮原子配位连接时，能显著恢复电子传递活性和放氧活性；三核锰化合物在重组时对CaCl2的存在非常敏感，我们认为Ca2 与三核锰化合物中的羧基之间存在一定的相互作用，而这种作用有助于锰原子的光配位进而使三核锰化合物易于组装成有活性的水氧化复合物：双核锰化合物MnMn比双核锰化合物MnMn在恢复放氧活性方面更有效；影响锰化合物电子传递能力恢复的因素与影响锰化合物放氧活性恢复的因素不同；在锰蔟重组过程中，氯离子的浓度必须在100mM以上，才能有效进行光重组；最大光重组效率的获得必须有钙离子和33kDa多肽同时存在；碳酸氢根离子促进锰化合物与去锰光系统II的光组装。

This paper discusses the research carried out on the Lake Sugan, in the northern margin of Chaidam Basin. The climate and environmental changes in Lake Sugan drainage area over the past 2670 years were studied using the varve dating of the well preserved lamination sediments in core SG03I taken from the center of Lake Sugan, the study of varve formation in modern lake system and modern process study of carbonate and oxygen isotopes of the water within the drainage area, and according to the main index of the stable isotopes of carbonate sediments synthesized other index analysis.

本文以柴达木盆地北缘苏干湖为研究对象，通过对湖泊中心位置SG03I孔保存完好的纹泥沉积物岩芯进行年代学研究，对湖泊现代沉积过程及碳酸盐、流域水体同位素进行现代过程研究，以沉积碳酸盐稳定同位素指标为主，结合其它代用指标的综合分析，探讨苏干湖流域近2670年来的气候与环境变化，综合区域其它记录对苏干湖流域晚全新世气候变化的驱动机制进行探讨，获得如下认识：1、柴达木盆地北缘苏干湖若干钻孔岩芯发现，水深大于3.0m的沉积物均具有裸眼可分辩的由深色层和浅色层组成的纹层层理。

DNA fingerprinting for Taraxacum mongolicum and its adulterants of six species of Compositae was demonstrated with random primed polymerase chain reaction including arbitrarily primed PCR and random amplified polymorphic DNA.

采用分子生物技术包括任意引物聚合酶链式反应和随意引物扩增的多态性DNA方法扩增蒲公英及其6种土公英混淆品的基因组DNA，获得清晰可靠的DNA指纹图谱。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。