获得物

In the third step, when the ratio of racemic ester to benzenesulfonyl chloride to triethylamine to catalyst 4-DMAP is 1:1:1:0.1 in 10℃, the yield is 82.9% and high purity is obtained by crystallisation.

在第三步磺酰化反应中，得到合成消旋2-苯磺酸基-2(2-氯苯基)乙酸甲酯的较佳反应条件为邻氯扁桃酸甲酯：苯磺酰氯：三乙胺=1：1：1，催化剂4-DMAP用量为摩尔反应物的10%，反应温度为10℃，收率为82.9%，同时用结晶方法获得高纯度的产物。

The QSAR model of multiple descriptors was established by the above parameters and the parameters of ...

在分类建模的基础上，又获得了仅包含1XV和EHOMO两个参数的55种取代苯系物的定量结构-活性相关模式。

The compound tray holds excellent features , it has been successfully used in industry namely , methane alcohol ethanol , methyl aeetyl distillations and etc It is also expected to have a broad prospect for applications in other chemical industry field In this paper , many studies are concerned with the gas liquid duallowing the hole and oscillating regulation of the compound tray by experiments These studies will provide theoretical and experimental foundation for the design and improvement of the compound tray The main part of which is a 500 mm inner diameter three compound trays column , serving as a hydrodynamic model , the system air ?

复合塔板以其优良的性能，在甲醇、乙醇和丙酮等生产精馏装置中获得了成功的应用，并在其它化工生产领域有着广泛的应用前景。本论文旨在通过实验研究复合塔板上气液穿孔流动及波动波动规律，为复合塔的设计和结构改进提供理论和实验依据。实验是在一塔径500mm的冷模塔内，以空气—水为物系，在常压下进行实验。

To obtain the VPg gene of PRSV-W, the VPg gene of PRSV-P was modified into a VPg gene of PRSV-W by long primer extension and overlap extension PCR.

以此为基础，利用长引物延伸和套叠PCR的方法，定点突变剩余5个位点，最后拼接获得W型PRSV-VPg基因。

Four repeatably differential expression fragments were reclaimed from the polyacrylamide gels, re-amplified, cloned and sequenced.

获得5对能够扩增出5条对小麦抗叶锈病近等基因系TcLr41具有特异性条带的引物，分别是P-AC/M-CAA、P-AG/M-CAT、P-AG/M-CCC、P-CC/M-CCA、P-GA/M-CAA。

According to the orthogonal experimental design, the optimum hydrolysis condition of porcine hemoglobin by combinative enzyme treatment are as foll...

通过正交实验，确定复合酶水解的最佳工艺条件为底物浓度10%、加酶比例3∶7、pH7.5、水解温度50℃、酶浓度8000U/g、水解10h，在此条件下获得的水解率为19.1%。

The apxⅢA gene of Actinobacillus pleuropneumonie was amplified by PCR. The amplified DNA fragment 3 466bp was cloned into pMD18-T. After R.E. analysis and sequencing, the apxⅢA gene in pMD18-T was ligated into pBluescripⅡSK, the recombinant expression plasmid pET-28b/apxⅢA was constructed and analysed with R.E., the protein of apxⅢA gene expressed in E.coli BL21 was detected by Western blotting.

因胸膜肺炎放线杆菌的致病性主要是由毒素决定的，故参照猪胸膜肺炎放线杆血清2型菌株的序列(GenBankL1 2 1 4 5)设计了一对特异性引物，用PCR的方法扩增apxⅢA基因并得到了长 3 466bp的片段，然后将其克隆到pMD 1 8T中，经酶切鉴定和序列分析表明克隆是成功的；再将apxⅢA插入到原核表达载体pET 2 8b后，转化BL2 1 (DE3)，在IPTG诱导下获得高效表达，经Westernblotting检测证实表达产物有活性。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。