获得物

A screening and cultivation method for Bacillus thuringiensis of high-yield high temperature resisting protease includes the following steps: high-yield high temperature resisting protease Bacillus thuringiensis Bt140 is obtained through adopting casein substrate agar to identify flat primary screen and fermenting enzyme activity measuring secondary screening; Plackett-Burman design and response surface analytical method are adopted to obtain optimal fermentation zymogenic formula and fermentation condition for Bt140 quickly; finally, shaking fermentation level reaches to 918.56U/mL.

一种高产耐高温蛋白酶的苏云金芽孢杆菌筛选及培养方法，采用酪蛋白底物琼脂鉴定平板初筛与发酵酶活测定复筛的方法获得高产耐高温蛋白酶苏云金芽孢杆菌Bt140；采用Plackett-Burman设计与响应面分析法快速获得了Bt140最佳的发酵产酶配方与发酵条件，最终摇瓶发酵水平可达918.56U/mL。

In this study,one pair of specific primer for mature chicken interleukin-18(ChIL-18) cDNA was designed and synthesized according to the previously published gene sequence of ChIL-18.The full length cDNA gene of ChIL-18 encoding mature active protein was amplified from LPS–stimulated MDCC-MSB1 cells by Reverse Transcription-Polymerase Chain Reaction.Then it was cloned into pMD18-T vector. Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

本研究根据已发表的ChIL-18成熟蛋白(mature ChIL-18，mChIL-18)的cDNA基因序列，设计一对特异性引物，应用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction，RT-PCR)技术从脂多糖刺激10小时活化的马立克氏病成淋巴细胞样细胞系MDCC-MSB1的cDNA中扩增出编码鸡IL-18成熟活性蛋白的全长基因，对扩增片段进行T-A克隆，经PCR、酶切鉴定及测序验证，成功获得ChIL-18成熟蛋白完整基因的克隆。

We can see that these come to the same thing, since if our access (to an external object, for example) is direct in sense (1), then there is nothing (at the intentional and personal level) to which we have access and in virtue of which we have access to the object.

我们能够明白这些获得的结果时一样的，因为如果我们在1意义上是直接可达的，那么在意向和人格水平，我们可以说，没有东西会是我们拥有的可达物且借助它能够可达对象。

When the SEBS content was 4%, the kaoline had little effect on the mechanical, morphological and rheological properties of the asphalts.

当LDPE复合物中白炭黑的份数为60时，可以获得高温贮存稳定的聚合物含量为3%～5%的改性沥青。

The 210Pb chronologies were corrected using the 1963 137Cs marker in Tauihu Lake core. The results show that sedimentation flux rates are very high, reaching to 0.6 g·cm-2·a-1 near 1990's in Taihu Lake.

对太湖钻孔利用137Cs 1963年对应的蓄积峰进行校正，采用210Pb计年的CRS模式获得不同时段的沉积速率发现在80年代末尾沉积物堆积通量最高，达到0.6 g·cm-2·a-1。

It is shown that uniform powder cloth with proper thickness can be produced by using appropriate rolling parameters. Thermogravimetic and pyrolysis residuum analysis indicate that the burnout process of fugitive binder used to produce the cloth is found to take place in two stages, no residuum is left when degassed properly.

结果表明，采用合适的轧制参数即可容易地获得厚度合适、均匀的粉末布；热失重分析和热解残余物分析指出用来制备粉末布的有机粘结剂的去除过程分成两个阶段，合理除气后，基本没有残余物。

In the fourteenth century, during the reign of Edward Ⅲof England, the pieces of cookware——iron pots, griddles, spits, and frying pans—were numbered among the king's jewels.

获得这些炊具颇不容易，它们因是稀世之物而显得极为贵重。

Internal dose ones and external dose ones are concluded.(2) Biomarker of effect. It indicates those indicaters inflecting the biochemical, biophysical, behavioral, or else changes. Three types of biomarkers, early biological effect, altered structure / functon and disease, are comprised.

生物标志物是包括反映生物系统与环境中化学、物理或生物因素之间相互作用的任何测定指标，可将其分为三类：(1)接触的生物标志物，即反映机体生物材料中外源性物质或其代谢物或外源性物质与某些靶细胞或靶分子相互作用产物的含量，包括反映内剂量和生物效应剂量两类标志物；(2)效应的生物标志物，指机体中可测出的生化、生理、行为或其他改变的指标，包括反映早期生物效应、结构和功能改变(altered structure/functon)及疾病3类标志物；(3)易感性的生物标志物，即反映机体先天具有或后天获得的对接触外源性物质产生反应能力的指标。

Acicular ferrite microstructure in micro-alloyed steels, which nucleates around non-metallic inclusions, especially oxide inclusions, has high strength and good toughness and possesses strong grain-refining ability by itself. The ultra-fine grained steel with excellent weldability is likely to be produced by forming in materials a great number of fine and dispersive micro-inclusions which is helpful to induce AF to nucleate around them through oxide metallurgy technology.

微合金钢针状铁素体组织强度高、韧性好，在奥氏体晶内非金属夹杂物上诱导形核，具有很强的自身细化晶粒的能力，通过氧化物冶金技术控制钢铁材料形成大量细小、弥散，具有诱导AF形核能力的有益微夹杂物，有望获得焊接性能优良的超细晶粒钢。

Guanosine overproducing mutant was derived from Bacillus subtilis T1001 by using ultraviolet and diethyl sulfate. The mutant was selected directionally and step wisely on the agar plates containing methionine sulfoxide and 8 azaguanine(8 AG).

以枯草芽孢杆菌T1 0 0 1为出发菌株，经紫外线、硫酸二乙酯逐级诱变处理，选育出腺嘌呤缺陷型菌株，然后经蛋氨酸亚砜、8 氮鸟嘌呤( 8 AG)结构类似物平板定向筛选，获得鸟苷高产菌株TA2 0 8。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。