获得物

Under the same conditions, three species were compared using fructose as substract for biotranformation to mannitol. Lactobacillus buchneri AS 1.13 has high rate of mannitol production.

在相同的条件下，比较了三种菌种以果糖为底物转化甘露醇，布氏乳杆菌AS1.13获得较高的甘露醇产率。

Using the crystal structure of CDK2 complexed with indirubin-5- sulphonate as the template, models of CDK4 complexed with indirubin and its analogue were built by homology modeling and molecular docking.

以获得晶体结构的CDK2与5-磺酸根-靛玉红的复合物为模板，通过同源模建和分子对接的方法构建出的CDK4与靛玉红及其衍生物的结合模式。

The similarity coefficients obtained from profiles generated by four primers among Thiobacillus Ferrooxidans were about 44%~83%.

通过4个引物的RAPD分析获得的平均相似性系数表明不同来源的菌之间的相关系数在44%~83%之间。

Trametes trogii was screened from Majia Hill in Weihai. The effect of pH, temperature and inorganic ion on Trametes trogii xylanase activity and stability was studied. Meanwhile, the substrate concentration effect of this xylanase was also studied and Km was measured.

从威海市玛珈山上获得毛栓菌，在对其产酶条件研究的基础上，主要研究pH、温度、无机离子对毛栓菌木聚糖酶活性和稳定性的影响，以及该酶底物浓度效应和Km值测定。

The sandwich-type immunocomplex was subsequently dissociated by the mixed solution of sodium hydroxide and trisodium citrate. The solution obtained, which contains gold nanoparticles coated with antibody, was used to quench fluorescence.

然后加入氢氧化钠和柠檬酸三钠的混合溶液解离免疫复合物，将获得的含有纳米金标记抗体的溶液进行荧光淬灭分析。

Low resolution was obtained in genetic variation investigation of different cowpea cultivated germplasm of Vigna unguiculata based on ITS sequence data, while good results could be obtained in genetic diversity and phylogeny of differern species of genus Vigna.

RAPD可以用于种下的遗传多样性研究。但基于随机引物的RAPD-PCR扩增，受实验中各种因素的影响较大，导致数据获得的重复性不是很好、且不可移植。因此只能作为一种辅助方法。从技术上讲，为保证实验条件的一致性，应该一次PCR反应中对所有的研究对象进行扩增，并在扩增片段的电泳分离检测时尽量保持条件的一致。

Four fractions of OTPS was obtained by DEAE-52 column chromatography, and there were obvious differences not only in contents of neutral sugar, uronic acid and protein of OTPS, but also in inhibiting effects on hydroxyl radical

获得的乌龙茶多糖主要组分OTPS2-1是富含糖醛酸和少量蛋白质的三元糖复合物，相对重均分子质量为8.877 10^4，糖基由半乳糖、葡萄糖、阿拉伯糖、岩藻糖和鼠李糖组成，其摩尔比为

The universal primers of ITS1 and ITS4 for fungi were used in amplification of genomic rDNA sequences. A target fragment was obtained by ITS-PCR. Then, the fragment is cloned into the vector of pMD18-T before sequencing. The result of sequencing showed that the fragment is 692 bp in length. The results of Blast in Genbank and the analysis of fungal rDNA systemic development show that the relation between this fragment to the fungi of Sporisorium is closest, while the relation between it to the fungi of Ustilago is much far. It suggests that the name of Ustilago scitaminea for sugarcane smut isn't consistent to the result of rDNA systemic development analysis. It still needs more experiments to support this hypothesis.

用真菌rDNA序列分析的通用引物ITS1和ITS4，通过ITS-PCR得到目的片段，克隆在pMD18-T载体上后进行测序，结果显示该片段的长度为692 bp，与Genebank中已报道的真菌序列进行Blast和系统发育树分析，表明所获得的序列与Sporisorium属真菌具有更高的亲缘关系，而与Ustilago属真菌的亲缘关系较远，这与一直沿用的甘蔗黑穗病菌的命名似乎并不吻合，有待进行更多的研究以证实。

Pattern of Candida species isolated from patients with diabetes mellitus and vulvovaginal candidiasis and their response to single dose oral fluconazole therapy ［J］.

同时MFC测定获得的结果也表明，利用更高浓度抗真菌剂稀释的标准液体培养基，极少能观察到完全抑制真菌的生长，但分子量为25和745ku的PEI对MY7238分离物的抑制则是例外。

A pair of primers were designed based on M1 gene sequence of known H5N1 Avian influenza virus.M1 gene was cloned from total RNA,extracted from tissue of H5N1 subtype virus inoculated embryo by reverse transcriptase-polymerase chain reaction using high proofreading polymerase(Pyobest~TM DNA Polymerase),and expressed using Invitrogen champion~TM pET directional TOPO expression system.Recombinant protein containing polyhistidine(6xHis) tag in N-terminal about 29.8kDa in size,wac obtained and purified.

根据已发表的禽流感病毒M1基因序列设计合成PCR克隆引物，自接种H5N1亚型病毒的鸡胚组织中提取RNA，反转录后采用高可信度DNA聚合酶经PCR扩增M1基因，采用Invitrogen定向表达系统(ChampionTMpET directional TOPO expression system)进行克隆表达，纯化获得N末端携带多聚组氨酸标签的重组蛋白，分子量约29.8 kDa。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。