获得物

Results Preparation of ss probes by asymmetric PCR and single-pr imer PCR gets success.

结果 不对称PCR及单引物PCR技术制备单链探针均获得成功；目的基因单链探针杂交条带清晰且几乎无背景值。

A series of acrylamide-styrene copolymer latices with different acrylamide and styrene weight ratio were prepared from the polymerization in emulsifier-free aquesous media .

为了在水介质中获得高固含量的聚丙烯酰胺，通过无皂乳液聚合制备了一系列不同单体比例的丙烯酰胺-苯乙烯的共聚物胶乳，固含量达50％，稳定性好，胶乳粒子大小均一，具有一定的触变性。

Compared with the conventional emulsion polymerization system,the latices that are of monodispersed latex particles of 0.5m m in diameter,low viscosity and high storage stability are prepared by emulsifier-free emulsion polymerization.

实验结果表明，与常规乳液聚合体系相比，所获得的无皂乳液的乳胶粒大小均一，粒径在0.5m m左右，粘度低，储存稳定；共聚物呈完全无规立构，玻璃化转变温度均在25℃附近，拉伸强度明显增大，耐水性和剥离强度显著提高。

For lensless magnification mode, the subtraction method was presented to eliminate the system total aberration to get the 3D phase information of the phase gating by using Fresnel approximation approach and the convolution approach, respectively.

在无透镜放大数字显微全息系统中，分别利用菲涅耳近似法和卷积方法再现原始物波前，并提出相减法消除系统主要球面误差，获得相位光栅的深度信息。

Specific primers were designed according to gene NHX1 of Limonium gmelinii published on NCBI. Then, the gene was cloned by means of RT-PCR. Eukaryotic expression vector pCAM-BIA1301-NHX1 had been constructed, then transformed into tomato by Agrobacterium-mediated transformation method. Transgenic tomatos were obtained.

根据NCBI公布的大叶补血草NHX1基因序列设计特异性引物，经RT-PCR方法克隆得到大叶补血草液泡膜Na/H逆向转运蛋白基因NHX1，构建植物融合表达载体pCAMBIA1301-NHX1，重组质粒通过农杆菌介导转化番茄，经PCR和RT-PCR鉴定，获得转基因番茄植株。

On the basis of whole grain evaporation age on single zircons using a double filament for different genetic zircons in metalassenite and metarhyolite of Luoyu formation,Yunkai group,the formation age of volcanic rock is 922 Ma;later geologic events is 643 Ma￣716 Ma or 427 Ma￣473 Ma...

本文采用颗粒锆石分层蒸发法分别对云开群罗罅组变英安岩及变流纹岩中不同成因锆石进行年龄测定，获得该火山岩之形成年龄为922Ma，940Ma；后期地质事件年龄为643Ma～716Ma，427Ma～473Ma及物源区的年龄为1616Ma，2084Ma。

The method comprises the steps that the molybdenum bearing spent catalyst is roasted and comminuted, then the molybdenum bearing spent catalyst is leach and filter by adopting alkaline solution bearing ammonia, ammonium bicarbonate and oxyful, and the secondary roasting and leaching are performed to the solids after filtration, the twice leaching solution is acidified and the aluminum is precipitated out, then is acidified continuously to precipitate out the molybdenum in the form of molybdic acid, the molybdic acid is dissolved by using ammonia water further, the pH value is adjusted again to obtain ammonium molybdate deposition, and the ammonium molybdate product is obtained after dryness.

将含钼废催化剂进行焙烧、粉碎，然后采用含氨、碳酸氢铵和双氧水的碱性溶液浸取并过滤，过滤后的固体物进行二次焙烧和浸取，两次浸取液酸化沉淀出铝，然后继续酸化将钼以钼酸形式沉淀出来，钼酸进一步用氨水溶解，再次调节pH值获得钼酸铵沉淀，干燥后得到钼酸铵产品。

Using cosmogenic determinations we have obtained the first in-situ morainal exposure ages from the glacial moraine in the Tanggula mountain and Litang.

本研究利用就地成因的宇宙核素暴露年龄测定方法，对唐古拉山和理塘地区的第四纪冰碛物进行了10Be、26Al和21Ne暴露年龄测定，首次获得了青藏高原的第四纪冰川核素暴露年龄。

The process of happiness is, firstly a sincerity heart is needed, getting ride of desire and being void and quiet; secondly to touch something nonego, and some feeling rising, at the same time the imaging activity must evoked enough; thirdly more image is needed, in order to find a world of significance, then to obtain a experience of the mysterious ultimate existence, which is the end of the aesthetic happiness with a mental mode named"wandering".

乐的过程，首先是要使其心诚，去欲而虚静；其次是要对外物有所感，并能兴发起一定的情感，与此同时想象活动要充分展开；再次，要进一步展开想象，发现一个意义世界，并由此获得对神秘的终极存在的体验，这才是以&游&的心意状态表现出的审美之乐的极至。

Eg95 antigen cDNAs were amplified by PCR from protoscolex, oncosphere and adult worm cDNA libraries of E. granulosus, respectively and were cloned into pUCm-T plasmid, and sequenced. The sequences were analyzed by DNAman and GenBank/BLAST biosoftware.

方法根据Eg95基因序列设计引物，用PCR方法分别从新疆株细粒棘球绦虫3个不同发育阶段所构建的cDNA文库中克隆获得Eg95目的基因，将其克隆至pUCm-T载体、测序并进行序列分析。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。