获得物

All PSI complexes could be solubilized by high concentration of Digitonin. Different area of thylakoid membrane could be separated by Tween-20 and PSI complex could be found in them solubilized by Digitonin.

高浓度的毛地黄皂苷能够获得全部的PSI复合物；Tween-20能够分离不同区域的类囊体膜，结合毛地黄皂苷处理均能够获得PSI复合物。

Two 15nt complementary ssDNA primers were ligated into double stranded satRNA.Complete sequence of satRNA was obtained by RT-PCR using these 15nt ssDNA as amplification primers.Phylogenetic analysis showed that the identity of this 383nt satellite and some documented satRNAs was 72.6 to 99.5% at the nucleotide level.

以常规引物对感病植物的总RNA进行RT-PCR扩增，获得CMV RNA3全长克隆，经测序显示该RNA3序列属于CMV亚组Ⅱ；通过在卫星dsRNA两端分别加上15nt的单链DNA接头，以接头互补序列为引物进行扩增获得一个383nt的卫星RNA的全长序列。

By employing such a method, 5 series of elastomers, including natural rubbers, elastomeric polyester fibers and ethylene-octene, ethylene-methyl acrylate as well as SIS triblock copolymers, were investigated with the purpose of studying elongation induced phase structure and mobility changes of the samples. For NR samples, it was demonstrated that there exists a small amount of crystals at RT even for unelongated samples. For both NR and the soft segment of PEE, with the increase of elongation ratio, the elongation-induced crystallinity increases almost linearly in a certain region of R, in accord with the increase of the lamellar thickness of the crystals; The behavior of ethylene copolymers, in terms of elongation induced morphorpholgy changes, are quite different from NR and PEE. The crystals are found to be gradually destroyed with the increase of R, with accord with the decrease of the lamellar thickness; The motional behavior of the soft segment of SIS, changes from "liquid" to "solid", in terms of 13C NMR spin-lattice relaxation.For many of the aformented samples, the dependence of 13C spin-lattice relaxtion time T1 on R are different for different groups, indicating that the changes of mobility at MHz frequency region with elongation are different for different groups.

通过对天然橡胶、聚醚酯嵌段共聚物、乙烯-辛烯共聚物、乙烯-丙烯酸甲酯共聚物、SIS等高分子弹性体样品聚集态结构和分子运动的研究，获得了一些新的实验结果，如：给出了确切的实验证据，证明了室温下未拉伸的天然橡胶样品中存在着微量的结晶；发现随着拉伸比增加，天然橡胶及聚醚酯样品的软段部分会出现拉伸诱导的结晶，且结晶度在一定范围内呈现近似线性的增加，所形成晶片的厚度也随之逐渐增加；而乙烯共聚物随着拉伸比的增加，原有的结晶会被逐渐破坏，晶片厚度逐渐变薄，结晶中的缺陷逐渐增加；随着拉伸比的增加，SIS中PI软段部分的核磁共振弛豫行为会出现从液态到固态的转变等；在对上述多个体系的研究中，还发现不同基团的自旋-晶格弛豫时间随着拉伸比呈现不同的变化趋势，表明不同基团的高频运动随拉伸比增加有不同的变化规律。

The RAPD was used to study the genetic diversity of 19 KinXin series potato cultivars. The result showed that 52 RAPD bands were amplified using 7 primer, among them 43 bands were polymorphic, and 4~9 bands were amplified in each lane. The RAPD analysis indicated that the genetic distance of 19 potato cultivars was between 0.17~0.72, average value was 0.39. When all of the polymorphic bands were analysed by cluster analysis, 19 potato cultivars were classified into 3 groups. The analysis showed that there existed close sibship among the majority of potato cultivars, and the genetic distance between the minority cultivars and the others is longer, which indicate that the genetic basis of KeXin series potato cultivars is wide.

利用RAPD标记技术对19份克新系列马铃薯品种进行了遗传多样性分析，分别提取19份马铃薯品种的DNA，进行随机引物多态性扩增，从1000条随机引物中初步筛选出7条有多态性的引物进行详细研究，每条RAPD引物扩增出4~9条带，共获得52条带，其中多态性条带为43条；19份马铃薯品种的遗传距离介于0.17~0.72之间，平均值为0.39，平均遗传距离介于0.31~0.51之间；聚类分析结果在GS=0.53处可将克新系列马铃薯品种划分为三类，聚类结果与系谱分析基本相符，同时也说明克新系列马铃薯的遗传基础有所拓宽。

ABSTRACT: Random amplified polymorphic DNA was used in analysing the relationship among twenty-six speices of Amanita from Mangshan, Hunan. Of 40 primers, 6 ones generated reproducible RAPD profiles, and each one produced 1-10 bands. Two primers (OPG15 and OPH04) could differentiate 26 species of Amanita by its RAPD profiles. The similarity coefficients obtained from profiles generated by six primers among Amanita were about 20-60%. A dendrogram was constructed using average linkage clustering for clustering analysis. It indicated that there were two groups in 26 species and some species with ring and membranous volva clustered a group which coincided with morphological classification.

本文对采自湖南莽山的26种鹅膏菌属真菌进行了随机扩增多态性DNA分析，40个随机引物中筛选出扩增效果较好的6个引物，每个引物能产生1～10条DNA条带，获得的RAPD谱带清晰并呈现多态性OPG15、OPH04两个引物扩增的RAPD谱带能将26种鹅膏菌完全区分开来，通过6个引物的RAPD分析获得的平均相似性系数表明种与种之间的相关系数在20―60%之间，平均链锁聚类分析可将26种鹅膏菌分为二大类，且一些具菌环和苞状菌托的种类聚在一起，与形态分类基本相吻合。

A total of 614 sediment samples at an interval of about 1.5 m from all 5 sites of the Integrated Ocean Drilling Program Expedition 311 on Cascadia Margin, northwestern Pacific were analyzed using a Beckman Coulter LS230 Particle Analyzer. The grainsize data were then plotted against depth and compared with other potential proxies of gas hydrateoccurrence such as soupy/mousselike fabrics in sedimentary textures, gas hydrate concentration derived from LWD data using Archie's relation, IR core images and the recovered samples of gas hydratebearing sediments.

对取自IODP 311航次（东北太平洋Cascadia大陆边缘）所有5个站位、采样间距约为1.5 m的614件沉积物样品，利用Beckman Coulter LS230激光粒度仪进行了沉积物粒度分析，获得了沉积物粒度随深度变化特征，进而与水合物层位的替代指标进行了位置对比，这些指标包括特殊沉积构造（soupy和mousselike构造）、测井数据推算出来的水合物饱和度、岩芯红外图像和实际钻取的含水合物沉积物等。

A total of 614 sediment samples at an interval of about 1.5m from all 5 sites of the Integrated Ocean Drilling Program Expedition 311 on Cascadia Margin, northwestern Pacific were analyzed using a Beckman Coulter LS-230 Particle Analyzer. The grain-size data were then plotted against depth and compared with other potential proxies of gas hydrate-occurrence such as soupy/mousse-like fabrics in sedimentary textures, gas hydrate concentration S(subscript h derived from LWD data using Archie's relation, IR core images and the recovered samples of gas hydrate-bearing sediments.

对取自IODP311航次（东北太平洋Cascadia大陆边缘）所有5个站位、采样间距约为1.5m的614件沉积物样品，利用Beckman Coulter LS-30激光粒度仪进行了沉积物粒度分析，获得了沉积物粒度随深度变化特征，进而与水合物层位的替代指标进行了位置对比，这些指标包括特珠沉积构造（soupy和mousse-like构造）、测井数据推算出来的水合物饱和度S(下标 h、岩芯红外图像和实际钻取的含水合物沉积物等。

Therefore, the LsHNXs genes were cloned in this study, and their function was studied by RNAi. The main results were shown as follows:1 The conservative sequences of three LsHNXs genes (LsHNX1, LsHNX2, and LsHNX3), and the 3'-terminal specific sequences of LsHNX2 and LsHNX3, were cloned by RT-PCR and 3'-RACE.2 The plant RNAi vectors were constructed and the transformation of Limonium sinense was done. The RNAi lines of three single-genes and one double-gene were obtained and were propogated rapidly for the molecular and the stress tolerance analysis.3 To be compared with wild type, the Na+ and K+ contents secreted by the leaves of LsNHX1 transgenic plants were remarkably higher.

主要实验结果如下：1采用RT-PCR和3'-RACE方法从补血草中克隆获得LsNHXs基因家族三个成员LsNHX1、LsNHX2和LsNHX3的保守区序列以及LsNHX2和LsNHX3的3'端特异序列。2构建植物基因RNAi沉默表达载体并转化补血草，获得三个单基因和一个双基因的RNAi沉默表达植株，快繁转基因植株供分子鉴定和耐逆分析。3LsNHX1转基因植株叶片表面分泌物中的Na+、K+离子含量比野生型对照显著增加；叶组织内Na+离子含量和K+离子含量均略高，但差异不显著；K+/Na+比没有显著差异。4LsNHX2转基因植株叶片表面分泌物中的Na+离子含量比野生型对照有所下降，但差异不显著；K+离子含量比对照显著降低。

Several novel structures have been determined: Unique ninenuclear complexesincorporating 3d, 4f metals with a sodium ion center, the sodium ion may be serve as atemplate in the formation of these novel complexes; Novel tetranuclear 〓complexes with channels formed by intermolecular weak interactions; First 〓type tetranuclear complex; Peculiar "dimer of dimers" complexes derived frombinuclear complex; Unusual one-dimensional wave chain formed by weakcoordination bonds and hydrogen bonds and two dimensional coordination polymerconstructed through coordination bonds and hydrogen bonds.

1获得了多个文献首报和罕见的特殊结构的配合物：两个同时含有碱金属-过渡-稀土金属的独特的九核配合物；两个具有孔道的稀土-过渡金属异四核配合物；草酰胺类〓四核配合物；两个由双核配合物通过叠氮和单个氯原子连成的四核配合物，尤其是以单个氯桥相连的化合物非常少见；一个由弱配键和氢键连成的具有一维波浪形结构的配合物；一个通过配位键和氢键连成的二维配位聚合物。

The Lab-3 high vacuum still is designed as a batch / continuous distillation unit.

LAB-3高真空蒸馏系统为成批/连续的蒸馏单元，参见上面的图表中看出，绿色为进给原料，蓝色为残留物，黄色为蒸馏获得物。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失