获得成功

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

In 1987 he launched his own production house QUARTIER LATIN specialising in Children's programs and Documentaries.

因此，米歇尔很快获得了巨大成功，这个团体也成为了国际性的法国出品公司，仅次于德国，英国和澳大利亚。1987年，米歇尔又创立了他自己的出品公司瓜地尔拉丁，主要从事于儿童节目和记录片工作。1995年，米歇尔又获得了优秀的发行公司ICTV。

The serious actress who won an Oscar for 1999's "Girl, Interrupted" and much acclaim for playing Mariane Pearl , widow of the murdered journalist Daniel Pearl , in last year's "Mighty Heart" is also the dominatrix-ish action dynamo who can open slam-bang guy movies, like this summer's "Wanted."

首先，她不仅是一位严肃的文艺演员，因为出演1999的《断了线的女孩》而获得奥斯卡奖，也因为在去年的《坚强的心》中成功地塑造了被害记者丹尼尔。帕尔的遗孀玛丽安。帕尔而获得高度的评价，同时，她还是一位首屈一指的动作片女星，能够在男性的天地里占据属于她的位置，就像今年夏天的《通缉今》所做到的一样。

"The Best of Years of Our Lives won best picture Oscar in 1946, and was just an all-around smash hit and success and it is still a wonderful film to this day."

"《黄金年代》在1946年获得了奥斯卡最佳影片，这部影片在当时大受欢迎，获得了全面的成功，至今看来它仍然是一部非常棒的影片。"

"Well,'' said Charlotte,"I wish Jane success with all my heart; and if she were married to him to-morrow, I should think she had as good a chance of happiness as if she were to be studying his character for a twelvemonth.

"唔，"夏绿蒂说，我一心一意祝吉英成功。我以为即使她明天就跟他结婚，她必能获得的幸福，比起她花上一年的时间，研究了他的性格、再去跟他结婚所能获得的幸福，并不见得会少到哪里去。

"Well," said Charlotte, I wish Jane success with all my heart; if she were married to him to-morrow, I should think she had as good a chance of happiness as if she were to be studying his character for a twelvemonth.

"唔，"夏绿蒂说，我一心一意祝吉英成功。我以为即使她明天就跟他结婚，她必能获得 s sbb ww 的幸福，比起她花上一年的时间，研究了他的性格、再去跟他结婚所能获得 s sbb ww 的幸福，并不见得会少到哪里去。

By coaxially detecting the images of the cutting front and the sparks jet , the relationship between the detected signals and cutting quality, including cutting defects and surface roughness, has been obtained.

本文通过同轴视觉传感系统同时获得切割前沿和切割火花簇射视觉图像，成功地提取了产生切割缺陷和切割面粗糙度变化时图像特征信号的变化规律，首次实现了以无切割缺陷并且在一定工艺条件下获得最高切割质量为目标的切割速度自寻优控制。

By coaxially detecting the images of the cutting front and the sparks jet, the relationship between the detected signals and cutting quality, including cutting defects and surface roughness, has been obtained. Self-optimizing control aiming to the best roughness on some certain cutting conditions has been realized.

本文通过同轴视觉传感系统同时获得切割前沿和切割火花簇射视觉图像，成功地提取了产生切割缺陷和切割面粗糙度变化时图像特征信号的变化规律，首次实现了以无切割缺陷并且在一定工艺条件下获得最高切割质量为目标的切割速度自寻优控制。

Since the 1997 release of the first novel Harry Potter and the Philosopher's Stone, which was retitled Harry Potter and the Sorcerer's Stone in the United States, the books have gained immense popularity, critical acclaim and commercial success worldwide.

自第一本小说1997年发行哈利波特与魔法石；这是改名；在美国哈利波特与魔法石；书获得了巨大的知名度；获得的好评和全球商业上的成功。

Research shows effective networkers tend to serve on more successful teams, get better performance reviews, receive more promotions and be more highly compensated.

研究表明有效地人际关系者往往会在更成功的团队里服务、获得更好的工作评估、获得更多晋升机会、和更高的报酬。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。