获得成功

We reconstructed the gene of human scFv and prepared scFv and scFv antibody fragments by prokaryotic expression system PET22b/BL21(DE3). After being purification by Ni-NTA column and renaturalization by urea dialyzation, the affinity and thermostability of scFv and scFv were detected.

结果表明，成功构建了scFv基因；实现了scFv和scFv在原核中的高效表达，二者的表达量均占菌体总量的35％以上，且成功的纯化了表达的重组蛋白，复性后获得了有活性的纯化重组蛋白。

The ectogenous gene TSLC1 has integrated into the genomes of T_3B cells which wereidentified from both nucleic acid and protein levels.

结果：(1)成功获得高表达TSLC1的稳定细胞系，核酸和蛋白水平鉴定都说明TSLC1基因成功整合到细胞基因组中。

You need personal courage, willingness, and determination to succeed, you need the support from your colleagues both vertically and horizontically to be more successful, and you also need the respect and acceptance from the business partners/whoever you are dealing with to be a sure success!

你要鼓起勇气，相信自己一定可以成功。同时，你还需要同级和上下级的同事们理解和支持。另外，获得生意合作者的尊重与接受是真正的成功！

RESULTS: High-purity ADSCs were acquired successfully. The ADSCs were induced into osteoplastic differentiation, and displayed typical adipocytes changes 7 days after induction. the cells turned to slender fusiform and mixed together, which showed positive PPARγ immunohistochemical staining and the lipid droplet was stained orange-red by oil red staining.

结果：①成功获得纯度较高的脂肪基质细胞，成功诱导其脂向分化，诱导培养后7 d观察到细胞形态学上表现出典型的脂肪细胞改变：细胞相互融合，成片生长，变为细长纺锤形；PPARγ免疫细胞化学染色呈阳性；油红O染色脂滴被染成橙红色。

This article selected the nine-dot problem as the material and investigated the inherent relation between the acquisition of insight in resolving the nine-dot problem and activation of heuristic information in prototypal matters through the learning and testing phases.

采用先学习源事件、后进行顿悟问题解决的两阶段实验范型，探讨了大学生解决九点四划问题的成功顿悟与源事件中所包含的启发信息提取之间的内在联系。结果发现，在顿悟问题解决阶段，只有激活学习阶段所获得的源事件中所包含的启发信息，才能成功解决顿悟问题。

Often the difficulties of growing up in the public eye cause child prodigies to retire from the world of achievement before reaching adulthood: happily, they sometimes later return to competition and succeed brilliantly.

在公众的注视下成长的窘境常常使得少年天才在成年前从成功人的世界中隐退，但幸运的是他们在一段时间后就又重回竞争并获得了灿烂的成功。

During the composition of this dissertation, all people in the world are focusing on the NASA's "Opportunity" and "Spirit" Rovers exploring the Mars and sending the data and pictures back to earth, and achieving great success. Many governments express their interesting to explore the outer space. It is clear that the telerobotics is the technical zenith which congregates both high technical talents and high technology and every governments chasing after.

在作者撰写论文的过程中，全世界都正在关注美国的两个火星探测车&机遇&号和&勇气&号登陆火星进行科学实验，并成功地回传了图像和实验数据，获得了巨大的成功，各国纷纷表示出探索外层空间的兴趣，可见遥操作机器人是当今高科技和高技术人才汇集、各国竞相抢占的技术制高点。

This is the standard that differentiates people who succeed in life and those who don't.

这是区别获得生活成功的人和不成功的人的标准。

When you come to the place of openly condemning the financial success that you crave, not only can that financial success never come to you, but you are also forfeiting your God-given rights to your health and happiness as well.

一旦你到了公开谴责你渴望的那种财务成功的地步，不光是那种财务成功不会来到你身边，而且你也是在放弃你获得健康和幸福的天赋权力。

Mama always gloried in any success I had, large or small, and they were mostly small.

无论我获得什么成功，大的还是小的，但差不多都是很小的成功，妈妈也都总会感到非常的光荣。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。