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Based on our results, 6 patients underwent reconstruction of the digital skin defects with the exposure of the bone or the muscular tendon using three kinds of venous flaps preserving the perivenous soft tissuepedical, the length of pedical was 1cm~3cm, the wide of pedical was 0. 6cm~0.8cm, the size of the flap was 1. 3cm×3cm~1. 5cm×6cm. All flaps survived, and satisfactory results were obtained in all these cases.

在上述研究的基础上,作者设计了三种类型的指背静脉皮瓣应用于临床,共10个皮瓣修复手指皮肤深度缺损6例,其中静脉周围组织蒂长1cm~3cm,静脉周围组织蒂宽0.6cm~0.8cm;静脉皮瓣面积1.2cm×3cm~1.5cm×6cm,术后皮瓣全部成活,获得良好效果。

In view of the theory and practice, multi-channel mode-filtered light CE has potentials in chiral separation of the biomolecules and the pharmic molecules, and in study of biological characteristics of the interaction of the bio-molecules with the pharmic molecules.

从理论和实验两方面来看,我们期望多通道模式滤光CE在生物分子和药物分子的手性拆分上、在研究生物大分子和药物分子之间相互作用的生物特征上,可以获得良好的应用。

As the development tendency of marine electrical propulsion, podded electrical propulsion have been widely applied abroad owing to its excellent characteristics.

作为船舶电力推进的发展方向,吊舱式电力推进以其优越的性能在国外获得了越来越广泛的应用。

Differing from the feed-back error compensation method, the selflearning error pre-compensation method offered by the paper is based on both ANN and time series technique to obtain the error pre-compensating curve using the non-linear ANN pre-compensation model. Because of the self-learning character of the ANN, this new type of precompensation model can be self-adjusted.

与常规的反馈补偿方法不同,本文提出的自学习误差预测补偿方法,将时间序列预测与神经网络的自学习自适应优化建模技术相结合,利用神经网络非线性预测模型产生误差预补偿函数,并利用神经网络的自学习特性对所建预测模型进行修正,所以这种新型的误差补偿方法能够获得更高的误差补偿精度和控制速度,简单实用,适于推广,具有生产应用价值。

Ground to prelect for many times, exchanges interview.

曾获得上海市重大科研成果三等奖。1987年研制的&慢性前列腺炎局部给药导管&系国内外首创,获国家发明三等奖,为前列腺炎治疗提供了一种新的治疗方法。1994年研制&助阳灵的研制及临床应用&获上海市卫生局重大科技成果三等奖。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

应用分子克隆技术,构建了斜带石斑鱼生长激素(Growth hormone,GH)基因重组质粒,在大肠杆菌细胞中获得高效表达,亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原,免疫家兔制备特异性抗体,通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交/核糖核酸酶保护试验及化学发光检测技术,研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制,对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion.

应用分子克隆技术,构建了斜带石斑鱼生长激素(Growth hormone,GH)基因重组质粒,在大肠杆菌细胞中获得高效表达,亲和层析纯化斜带石。。。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper,Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术,构建了斜带石斑鱼生长激素(Growth hormone,GH)基因重组质粒,在大肠杆菌细胞中获得高效表达,亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原,免疫家兔制备特异性抗体,通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交/核糖核酸酶保护试验及化学发光检测技术,研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制,对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Eleven of the twenty nine pseudopregnant recipient mice became gestation and fifty five offspring were produced.

将 16 只首建者小鼠分别与非转基因鼠交配,共获得 F1 代仔鼠 157 只。应用PCR 方法对 F1 代小鼠进行检测,结果首建者1 号母鼠所生的6 只仔鼠中有3 只为阳性。

Methods first , to acquire the highly pured glomeruli by three steps grade filtrated net ; next , to culture the microvascular endothelial cell in vitro with the transplant methods of isolated glomerali.

方法采用三步梯度筛网法,先获得较高纯度的肾血管球,再应用肾血管球外植法体外培养肾微血管内皮细胞。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力