获得应用

Were done and no positive reaction was found.5. The DNA of the young leaves of 132 Bt transgenic plants was extracted to do PCR Five positive inversion plants were obtained and the inversion rate was 3.8%.6. The DNA of fire positive inversion plants obtained in Dl generation was extracted and tested. Two stable positive inversion plants were obtained in D2 generations.7 The way of flourescent mirror flat was used to observe the pollen tube extension circumstance in the flower post after self-pollination.

检测了132株，没有发现阳性反应。5、对转入Bt基因的132株植株的幼叶提DNA，作PCR检测。D1代获得5株阳性转化植株，分别是90035的4株，85—593的1株，转化率为3.8％。6、将D1代获得5株阳性转化植株的种子在温箱中发芽，提取DNA检测，得到D2代稳定遗传的阳性转化植株2株。7、应用荧光镜制片法观察大豆自花授粉后花粉管在花柱中延伸情况。

In the first chapter (1.1.1)-(1.1.4) is studied by means of fixed point index theorem, not only the existence theorem is obtained, but the multiplicity is also obtained.

本文第一章利用这一理论研究方程(1.1.1)-(1.1.4)的正周期解问题，与已有研究成果相比，本章不仅获得了该类方程的正周期解的存在性定理又获得了正周期解的多解性定理，并将所取得的结论应用于一些具有实际背景的方程。

Analysis of the obtained genome amplification showed that DNA after PCR presented obvious polymorphism, and the results were the same using the same primer to amplify the larva and imago of the same species. It indicates that RAPD technique can be applied to the identification of dry samples and dipped larva sample of longicorn beetles as well as the research of phylogeny. These three methods for extracting genome DNA from different species of longicorn beetles at different preserved time are feasible.

对所获得的基因组扩增分析表明，经过PCR之后的DNA能呈现明显的多态性，同种天牛用同一引物扩增幼虫和成虫所获得的结果相同，可以认为，RAPD技术可以应用于天牛成虫干标本及幼虫浸渍标本的鉴定及系统发育研究中，也证明了这3种方法用于提取不同保存时间、不同种天牛基因组的DNA是切实可行的。

Lagrange multiplication operator was used to analyze the parameter estimation equation for obtaining the optimized estimation of parameters. Computer simulation result showed that this method is better than traditional least squares estimation.

计算机仿真的结果表明，该优于传统的最小二乘估计法，获得的估计参数是渐进无偏的，获得的估计参数的辨识精度更高，该参数约束估计法在系统辨识和参数估计领域具有极其重要的实际应用价值。

You may be wanting to get more creative with the space, or get as much information on-screen as possible.

您可能想获得更多的空间创意，或与Web应用程序获得尽可能多的资料，尽可能。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

In study of the truss structural dynamic characteristics analysis, randomness of the structural physical parameters and geomtric ones are all considered. The finite element model of the stochastic truss is built and the random functions of its natural frequencies are obtained by use of the Rayleigh quotient. Employing probabilistic distribution function expression for the random functions, through a series of mathematical processing including determination of limits of integrals, variable substitution etc., the probaabilistic density function for the truss natural frequencies are gained to realige accurate calculation for the reliability of the truss natural frequencies.

考虑结构物理参数和几何参数的随机性，通过构建随机参数桁架结构的有限元模型，利用瑞利商公式获得了结构固有频率随机函数的计算表达式，应用随机函数的概率分布函数表达式，通过确定积分区间、变量替换、积分顺序变换等一系列数学上的处理，获得结构固有频率的概率密度函数，进而实现对结构固有频率可靠度的精确计算。

There have been more than 110,000 students with the senior technology of engineering and the administration skill graduating from SUT within the 50 years since the founding of the university.

"十五"以来，学校共承担国家"863"计划、"973"计划、"十五"攻关、自然科学基金、社科基金等国家级计划项目122项，省部级等计划项目990项，企事业单位委托项目1635项，完成了一批获得社会认同的具有较高水平和较大应用价值的科学技术成果，共取得鉴定成果、国家专利493项，获得省部级以上科技奖励159项。

Chapter 2 is devoted to study of exact solutions of the nonlinear evolution equations. Using solutions of a Bernoulli equation instead of tanh in tanh-function method we find some more general solutions of the KdV-Burgers-Kuramoto equation , and by using the nonlinear telegraph equation we show that there are many different choices on its balancing number m and the power n of the nonlinear term in Bernoulli equation by which we can recover the previously known solutions and also can derive new square root type solitary wave solutions. Exact solitary wave solutions for a surface wave equation are obtained by means of the homogeneous balance method. We also present an approach for constructing the solitary wave solutions and non-solitary wave solutions of the nonlinear evolution equations by using the homogeneous balance method directly, which is also used to find the steady state solutions, solitary wave solutions and the non-solitary wave solutions of the 2+1 dimensional dispersive long wave equations. The soliton-like solutions of the BLMP equation and the 2+1 dimensional breaking soliton equation are found by use of the symbolic-computation-based Method.

第二章中研究了非线性发展方程的精确解：用双曲正切函数法中的双曲正切函数换为Bernoulli方程的解的方法而给出KdV-Burgers-Kuramoto方程的精确解并用非线性电波方程为例说明了平衡数m和Bernoulli方程中非线性项的次数n有着多种选择的可能，它不但使我们能找到已知解而且也能找出新的根式孤立波解；用齐次平衡法给出一个曲面波方程的精确孤立波解，并提出直接用齐次平衡法寻找非线性发展方程的孤立波解、非孤立波解的方法，作为应用给出2+1维色散长波方程组等的定态解、孤立波解、非孤立波解等；用Symbolic-computation-basedMethod获得BLMP方程和2+1维破裂孤子方程的类孤子解；提出sine-Gordon型方程的直接求解方法，并获得sine-Gordon方程、双sine-Gordon方程、sinh-Gordon方程、MKdV-sine-Gordon方程和Born-Infeld方程等的精确孤立波解。

By full use of the test data, the best saccharification technology of glutinous rice by two enzymes method in monascus waxberry wine was obtained.

提出了一种将人工神经网络技术与传统正交试验方法相结合的试验数据分析、处理和优化的新方法，能充分利用试验数据，仿真获得最优组合，并将该方法应用到红曲杨梅果酒的糯米淀粉双酶法糖化工艺中，获得了最佳工艺，取得了很好的效

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。