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Methods Indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of Aspergillus fumigatus,respectively.Two different double monoclonal antibody sandwich ELISA assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of Aspergillus,Penicillium marneffei,and 5 species of Candidas.

采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心ELISA法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

The election was too close for our side to prevail in Indiana, but we carried Arkansas with 65 percent, just two points less than President Carters 67 percent margin in his native Georgia and seven points better than the next largest victory margin, in West Virginia.

选举的结果非常接近，因而我们一方在印第安纳州没有取胜，但是我们在阿肯色州以65％的得票率获得了多数选票，这与卡特在他的老家佐治亚州所获得的67％的得票率相比，只低了两个百分点；与取得第三大获胜优势的西弗吉尼亚州相比，则高出七个百分点。

In 1898, the Curies obtained a new element, whose radioactivity was several million times stronger than that of uranium.

1898年，居里夫妇获得了一种新元素，它的放射性比铀强几年居里夫妇获得了一种新元素，百万倍。

Sequences were aligned by BLAST method. Results showed that 309bp fragments shared high similarity with orfl38 in Ogu CMS radish and Brassica cybrids of Ogu CMS radish, which 172 nucleotide sequences and 58 amino acids were same among them. And 689bp fragments shared high similarity (100%) with Pol orf224 in B.napus, which 677 nucleotide sequences and 225 amino acids were same among them.

同源性分析结果表明，利用orf138引物所获得的309bP大白菜mtDNA特异片段均与萝卜Ogu CMS、甘蓝型油菜Ogu CMS萝卜体细胞杂种所具有的Ogu orf138高度同源，二者有172个核苷酸完全相同，有58个氨基酸完全相同；orf224引物所获得的689bP大白菜mtDNA特异片段与甘蓝型油菜的Pol orf224高度同源，二者有677个核苷酸完全相同，有225个氨基酸完全相同，同源性均达到100%。

Result］four of 9 patients successfully retained their component at most recent follow up, without ongoing infection (mean 18 months, range 10～25 months).we have not draw the statistically significant factors that related to successful and failure,but the factors such as acute infection, surfacing replacement, immediate debrided after onset of symptoms, and the bacteria sensitive to antibiotic were relate to success.

由于病例数较少，本研究针对可能影响清创成功的因素未能得出有意义的统计学结果，但从结果看：急性感染清创容易成功，而慢性感染则很难获得成功；表皮葡萄球菌成功率较高，金黄色葡萄球菌均失败；表面膝置换成功率较高，而铰链膝置换则难以获得成功；出现症状后应该抓紧时间进行清创，延误的时间越长，成功的可能性越低。

Eligible personnel who had been mentioned in despatches during the War were entitled to wear a bronze oak leaf emblem on the ribbon.

这个勋章受给条件：从1939年9月3号到1945年9月2号内，全职服役人员并且服役超过28天的。必须是已经获得战役星型勋章，并且是战死，战伤，或者被敌人俘获的才有资格获得这枚勋章。

The reinforced nanocomposites are usually prepared by dopping particles or fibers with nanometer scale in matrix and then sintering under vacuum or by precipitating nanophase from matrix.

颗粒增强纳米复合材料一般将具有优异力学性能的纳米颗粒或纤维掺入到基体材料中，再经在真空等静压烧结获得，或者控制热处理条件使基质晶淀析出纳米晶第二相而获得。

Analysis indicates that the adjacent channel power is a quadratic function of predistorter efficients. A new optimization method is given which uses the predicted ACP surface to obtain fast convergence and simulation results show that a 20dB reduction in the third IMD and a 10dB reduction in the quatic IMD are achievable.

得出结论为：分析表明功放输出的邻信道功率是预失真器系数的二次函数；利用了新的自适应算法即使用预测的ACP曲面以提供更快的收敛速度；仿真结果表明，3阶产物可获得20dB以上的改善，5阶产物可获得10dB以上的改善。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。