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Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库；(2)经蓝白菌落筛选和斑点杂交，正向消减文库获得307个阳性克隆，反向消减文库获得78个阳性克隆；(3)正向消减文库挑选55个克隆成功测序，经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95％～100％)，这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等；(4)反向消减文库挑选31个克隆成功测序，经同源性检索和比对最终确定差异表达基因片段16个，其中15个与人类全长基因具有很高相似性(95％～100％)，包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性，表明它可能是未被克隆的人类基因EST片段。

He thinks, had passed to rely on taletelling to move now the time of investor, the person that do poineering work wants to do, more it is to make good product, acquire an user, final ability gains the favour of investor.

他认为，现在已经过了靠讲故事打动投资人的年代，创业者要做的，更多的是做好产品，获得用户，最终才能获得投资人的青睐。

When the application for tax and duty free allowance submitted is approved, the Thai National Commission for UNESCO will notify Customs of the approval, including the list of goods to be granted tax and duty free entry and the name of importing institutes/government agencies.

当加税和免税津贴提交的申请获得批准，泰国教科文组织全国委员会将通知海关的批准，包括货物清单，以获得税收和关税自由进入和进口机构的名称/政府机构。

One view is that the nucleocapsids get tegument in the nucleus, and by budding into perinuclear space, they obtain their envelope from the inner lamella of the nuclear membra...

一种意见认为，核衣壳在细胞核内获得被膜，并通过出芽在内层核膜处获得其囊膜后，进入核周间隙，进一步经与核周间隙相连接的内质网，以囊泡转运的方式经高尔基体到达细胞膜，囊泡膜与细胞膜融合，病毒颗粒释放到细胞外。

Chapter 2 is devoted to study of exact solutions of the nonlinear evolution equations. Using solutions of a Bernoulli equation instead of tanh in tanh-function method we find some more general solutions of the KdV-Burgers-Kuramoto equation , and by using the nonlinear telegraph equation we show that there are many different choices on its balancing number m and the power n of the nonlinear term in Bernoulli equation by which we can recover the previously known solutions and also can derive new square root type solitary wave solutions. Exact solitary wave solutions for a surface wave equation are obtained by means of the homogeneous balance method. We also present an approach for constructing the solitary wave solutions and non-solitary wave solutions of the nonlinear evolution equations by using the homogeneous balance method directly, which is also used to find the steady state solutions, solitary wave solutions and the non-solitary wave solutions of the 2+1 dimensional dispersive long wave equations. The soliton-like solutions of the BLMP equation and the 2+1 dimensional breaking soliton equation are found by use of the symbolic-computation-based Method.

第二章中研究了非线性发展方程的精确解：用双曲正切函数法中的双曲正切函数换为Bernoulli方程的解的方法而给出KdV-Burgers-Kuramoto方程的精确解并用非线性电波方程为例说明了平衡数m和Bernoulli方程中非线性项的次数n有着多种选择的可能，它不但使我们能找到已知解而且也能找出新的根式孤立波解；用齐次平衡法给出一个曲面波方程的精确孤立波解，并提出直接用齐次平衡法寻找非线性发展方程的孤立波解、非孤立波解的方法，作为应用给出2+1维色散长波方程组等的定态解、孤立波解、非孤立波解等；用Symbolic-computation-basedMethod获得BLMP方程和2+1维破裂孤子方程的类孤子解；提出sine-Gordon型方程的直接求解方法，并获得sine-Gordon方程、双sine-Gordon方程、sinh-Gordon方程、MKdV-sine-Gordon方程和Born-Infeld方程等的精确孤立波解。

Tensile and compression tests are carried out to reveal the mechanical properties of different samples.

将获得的试样进行拉伸和压缩变形，获得不同试样的力学性能。

Cm tensiometer is using relative number that represents a tension can be defined by its own weight tensiometer so that the depth of the screen and handlethe, tension values may be indicated on the disk, or from directly from contropanels on indirectly.

厘米压力仪就是用不绝对数值表现压力的一栽，是堵住压力仪自身轻量使丝网下沉的广度的数值来筹算，压力数值可从辅导盘上不直接获得，或者从把持盘上不直接获得。

Applied DWT to R,G,B channel correspondingly, and used three low frequent coefficients to construct a 3-level tensor. Embedding the watermark into the core tensor, which got form Tucker decomposition, could diffuse the watermark into R,G,B.

首先对彩色图像R、G、B三通道进行小波变换，然后将获得的三个低频系数构成三阶张量，再经过Tucker分解后获得核心张量，通过在核心张量中嵌入水印信息使得水印信息扩散到原彩色图像的R、G、B三个分量中。

This serveth in addition (to the effects of the two preceding) to acquire Kingdom and Empire, to inflict loss, and to acquire renown and glory, especially through the Name of God, Tetragrammaton, which therein is twelve times contained.

这个用来附加获得王国和君权，造成损失，和获得名声与荣耀，特别是通过在那里重复12次神之名Tetragrammaton。

The Chinese teen made her world championships debut this year, winning a team silver medal and placing fourth in the floor exercise.

这也是中国队获得的今年以来的第一个冠军。中国队还获得了团体银牌和自由体操第四名的成绩。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。