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The adenovirus plasmid was identified by PacI digestion and transfected into 293A cells to package a recombinant adenovirus which expressed the Fhit protein. Furthermore, the adenovirus rAd-Fhit was infected into colon cancer cells,and the expression of the ectogenic protein was detected by Western blotting. Finally, the proliferation of colon cancer cells was observed in adenovirus-infected cells by the MTT assay.

经PacI酶切鉴定正确后，将重组腺病毒质粒转染293A细胞获得表达Fhit蛋白的重组腺病毒rAd-Fhit，将获得的重组腺病毒感染结肠癌细胞，采用蛋白印迹法检测外源Fhit蛋白的表达，并进一步观察其对细胞增殖能力的影响。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

A method is therefore proposed to change the grinding wheel edge from parallel into inclined form so as to optimize the structure of abrasive layer.

点磨削砂轮磨料层采用有粗磨区修整倾角的结构可获得良好的磨削性能，较传统砂轮磨料层结构可获得更理想的磨后表面。

The mount of B16 PELs from〓cells/mouse onfirst day augmented to〓/mouse on sixth day.

不同诱导天数内收集的〓PEL与mIL-2〓PEL用〓释放试验分析其杀伤百分率的动态变化，mIL-2〓PEL的杀伤百分率从第1天时的0跃到第2天的22.5%，随后就维持在一个相对稳定的平台期，〓PEL从第1到第3天的杀伤百分率均为0，从第4天开始获得3.4%，到第5天才获得12.1%的杀伤率随后进入平台期。4。

Compared with Huaqiao University Basketball Team,the other three teams have some obvious weaknesses on the shooting percentage of hits,penalty shot percentage of hits,the attack ability,etc.

本文通过对第八届CUBA东南赛区男子组获得前4名球队比赛临场统计指标的分析，揭示大学生篮球联赛的发展状况，为CUBA教练员提供新的训练比赛信息。1研究对象与方法以第八届CUBA东南赛区男子组获得前4名球队为研究对象。事先就有关研究问题查阅了相关专业书籍、论文和资料，为本文研究提供理论支持。

The 5th website obtains click the 4th Http://flash.5617.com /(to be in standard browser interface, google searchs result page the first screen is to show 5 results, this one result is clicked with the 10th be more than the 9th to make clear together, head the attention that the last result gains screen is the) that is more than penult.

第五名网站获得的点击超过第四位http：//flash.5617.com /(在标准浏览器界面中，Google搜索结果页面第一屏是显示5 个结果，这一结果与第十名点击大于第九名一起表明，首屏最后一个结果获得的注重力是大于倒数第二位的)。

When he went to Petersburg he was received as he deserved.

他到彼得堡以后，他所获得的只是他理应获得的一切。

Some get free drinks, others get free meals, and all get a chance to step off the ship for the main reason Petty Officer Kidd is here.

一些人获得了免费的饮料，另一些则是免费的食物，不过所有士兵都获得了下船的机会，这主要得归功于船上的 Kidd 士官。

A complete phase diagram is obtained;The scalingbehavior in the quantum critical region of the Néel phase boundary of the bilayerJ1-J2 model is obtained for the first time.

获得了此模型完整的相图，并第一次获得了此模型在量子临界区的标度行为。

The construction characteristics of calcium phosphite were measured with Y-4Q system X-ray diffraction apparatus and the thermal analysis method in this paper.

本文采用热分析和x衍射方法研究了亚磷酸氢钙的结构特性，获得了亚磷酸氢钙结晶水合物的热分解特性和有关热分析数据；获得了亚磷酸氢钙结晶水合物的x衍射图谱和有关衍射数据；分析了它们与结构及其变化的关系。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。