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Hairy roots were induced from tobacco leaves with Agrobacterium rhizogenes LBA1334 harboring agropin type PRil855 and binary vector PIG121HM carrying a kanamycin resistant gene nptll and GUS gene and the good hairy root clones were selected. The root with many root hairs and branches grew vigorously toward all the direction on MS medium without plant hormone and on kanamyain containing medium (km:30mg/L). The structure of the hairy root tip is different from that of ordinary root. Witch was not typical root cap maybe the main cause of losing the geotropism of hairy root. Plantlet regenerated from hairy root when cultured on Ms medium without plant hormone. The nicotine content of the hairy root was little higher than that in the natural roots.

本研究通过发根农杆菌工程菌LBA1334(含有野生型pRi1855质粒和pIG121HM双元载体质粒，编码NPTⅡ基因和GUS基因)转化烟草沙姆逊，获得了具有卡那霉素抗性烟草发状根，并筛选出良好的单克隆株系，该发状根具有典型的发状根特性(多根毛、多分支、在无激素培养基上快速生长)，通过根尖压片分析，烟草发状根不具备典型的根冠；发状根中烟碱含量1.61％稍高于与天然栽培品种母体根的含量(1.5％左右)，发状根向培养液中释放烟碱，释放量最多时占总烟碱量的81.1％；在无激素的培养基上获得了再生植株。

The embryos obtained germinability earlier than the desiccation tolerance during maize seeds development.

发育过程中玉米胚萌发能力的获得早于耐脱水能力的获得，但二者在时间上存在较大的重叠。

Nouns ---verbs gerund examples One of our ways for getting heat is burning fuels.

我们获得热的一种方式是燃烧燃料。我们获得热的一种方式是燃烧燃料。

But let''s get one thing straight…pheromones alone won"t get you across the line with a woman. Maybe some of you are thinking…"Heck, I just want to get the opportunity to get into a meaningful conversation with a girl let alone hit a home run!

"但let'的获得一件事，直…费洛蒙，仅赢得了"吨让您跨越蓝线与一名女子，也许你们当中有些人是思想……"赫克，我只想获得机会进入一个有意义的对话与一名女童更遑论击出了本垒打"！

In painting she tries all kinds of methods – including chickens, watermelons, bad taste, dirty paint, wisecracks, the mundane, commercial paintings, pretense, cheapness, clumsy imitation, girlishness, etc – to make a mockery of painting.

于是，绘画在自嘲中获得了解脱，对，在绘画中想尽各种办法——包括鸡、西瓜、坏趣味、脏颜料、插科打诨、俗、行画、装腔作势、廉价、幼稚的模仿、小女人气等等——来嘲笑绘画，这正是段建宇使绘画获得自由的办法。

Indeed, other local organic growers with whom the Financial Gleaner spoke note that they have received certification in several European countries, but none from the Coffee Industry Board.

事实上，Financial Gleaner 曾经采访的本地其它几家有机种植者都声称他们是从几个欧洲国家获得此类证书，而不是从牙买加咖啡工业委员会获得。

No He won't have it , he knows his whole back city's ropes It don't matter, he's dope He knows that, but he's broke He's so stacked that he knows When he goes back to his mobile home, that's when it's Back to the lab again yo This whole rap shit He better go capture this moment and hope it don't pass him You better lose yourself in the music, the moment You own it, you better never let it go You only get one shot, do not miss your chance to blow This opportunity comes once in a lifetime yo The soul's escaping, through this hole that it's gaping This world is mine for the taking Make me king, as we move toward a, new world order A normal life is borin, but superstardom's close to post mortar It only grows harder, only grows hotter He blows us all over these hoes is all on him Coast to coast shows, he's know as the globetrotter Lonely roads, God only knows He's grown farther from home, he's no father He goes home and barely knows his own daughter But hold your nose cuz here goes the cold water His bosses don't want him no mo, he's cold product They moved on to the next schmoe who flows He nose dove and sold nada So the soap opera is told and unfolds I suppose it's old potna, but the beat goes on Da da dum da dum da da You better lose yourself in the music, the moment You own it, you better never let it go You only get one shot, do not miss your chance to blow This opportunity comes once in a lifetime yo No more games, I'ma change what you call rage Tear this mothafuckin roof off like 2 dogs caged I was playin in the beginnin, the mood all changed I been chewed up and spit out and booed off stage But I kept rhymin and stepwritin the next cypher Best believe somebody's payin the pied piper All the pain inside amplified by the fact That I can't get by with my 9 to 5 And I can't provide the right type of life for my family Cuz man, these goddam food stamps don't buy diapers And it's no movie, there's no Mekhi Phifer, this is my life And these times are so hard and it's getting even harder Tryin to feed and water my seed, plus See dishonor caught up bein a father and a prima donna Baby mama drama's screamin on and Too much for me to wanna Stay in one spot, another jam or not Has gotten me to the point, I'm like a snail I've got to formulate a plot fore I end up in jail or shot Success is my only mothafuckin option, failure's not Mom, I love you, but this trail has got to go I cannot grow old in Salem's lot So here I go is my shot.

无 他不会有，他知道他的整个回城的绳索它不处理此事，但他的涂料他知道，但他的突破他的，所以堆叠他知道当他回到他的手机在家，就什么时候，它的回到实验室又摇摇这整个说唱为Shit 他最好去捕捉这一刻，并希望它不通过他您更好地失去了自己在音乐，时刻你拥有它，你最好永远不会放手你只能获得一杆，千万不要错过你的机会，以打击这个机遇而来的一生中只有一次的心灵灵魂的逃亡，透过这个洞，它的溃烂这个世界是矿井为录取令我国王，因为我们走向一个新的世界秩序过正常的生活，是borin ，但superstardom的接近邮政砂浆它不仅日益困难，只有长大白热化他的打击我们所有这些锄头，是所有对他的海岸到海岸的表演，他知道作为globetrotter 寂寞的道路，只有上帝知道他的成长到离家远，他的父亲并没有他回家，勉强知道自己的女儿但对于你的鼻子cuz这里有云冷水他的老板不希望他没有谟，他的冷战产物他们转移到下一schmoe谁流向他的鼻子鸽派和出售纳达所以肥皂歌剧是告诉，并逐步落实我想它的旧potna ，但在街上巡逻下去大达达姆弹达达姆弹达大您更好地失去了自己在音乐，时刻你拥有它，你最好永远不会放手你只能获得一杆，千万不要错过你的机会，以打击这个机遇而来的一生中只有一次的心灵没有更多游戏，我是改变你们称之为愤怒催泪这mothafuckin屋顶起飞一样二日犬笼我是playin在beginnin ，心情，一切都变了我是咀嚼起来，吐出一片嘘声起飞阶段但我一直rhymin和stepwritin未来的CYPHER 最好相信有人的payin该pied伯尔所有的痛苦内扩增事实我不能得到我的9至5月我不能提供适当类型的生命，因为我的家庭 cuz男子，这些goddam粮票不买尿布它的不看电影，有没有mekhi phifer ，这是我的生命这些时代，所以很难和它变得更难 tryin到饲料和水我的种子，加上见荣辱赶上了bein父亲和表面唐娜宝宝妈妈戏剧的尖叫和太多，我想留在现场的一个，另一个果酱或不得到了我的一点，我想蜗牛我必须制订一项阴谋前列我结束了在监狱或打针成功是我唯一的mothafuckin选择，失败的不是妈妈，我爱你，但这个线索已经去我不能老在塞勒姆的地段所以在这里，我去的是我的了一枪。

The expression vector pET32a-ITF was constructed and expressed in E.coli successfully, and the recombinant protein rITF and its antibody was also purified in this study. ITF expressed in small intestine goblet cell.

成功构建了表达载体pET32a-ITF，在大肠杆菌中表达并纯化获得纯度较高的rITF，并获得了生物活性较高的ITF抗体，ITF主要在肠道杯状细胞分泌表达。

Mizzou has a grant program for international students. The Curator's Grant-in-Aid program is for those who get good grades and take part in university activities. Graduate students who receive a grant get nine free credits to take courses. Undergraduates receive between one thousand and five thousand dollars in support.

Mizzou 提供一项针对留学生的补助金计划，这项管理者补助金计划是专门针对那些成绩优良并且积极参与大学活动的留学生，接受这项补助金的研究生将获得 9 分的免费选修课程，而本科生将获得 1000 至 1500 美元的直接经济资助。

There is some speculation of both diesel and hybrid variants eventually getting the greenlight, while a biofuel model may be immediately available.

有一些投机两个柴油和混合变种最终获得了绿灯，而生物燃料的模型可立即获得。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。