荷叶

Regardless of the kind of competition, lotus leaf fern Adiantum in 5400 lux light intensity, 60 percent of soil moisture conditions, the total biomass of the largest, which also shows a lotus leaf fern Adiantum more to live in this habitat, but In such a habitat, the species of lotus leaf fern Adiantum competition among the lowest relative intensity, but these habitats and less dispersed.

无论有无种内竞争，荷叶铁线蕨在5400lux光照强度，60%土壤含水量的环境条件下，总生物量最大，这也说明了荷叶铁线蕨更适应生活在这个生境中，但是在这种生境，荷叶铁线蕨的种间竞争相对作用强度最低，而且这些生境少并分散。

Through research, give the following conclusion: there is no competition, lotus leaf fern and wire fern Adiantum as drought intensifies, the total biomass of a decreasing trend when the water content at the same time, with the intensity of Increase in total biomass showed an increasing trend, LAR general with the increase in soil moisture and lower, with light to reduce the increase, when in a kind of competition within and between species, the lotus leaf fern Adiantum the total biomass is still followed Such laws, but because the resources snatch the total biomass decreased significantly.

通过研究，得到以下结论：在不存在竞争的情况下，荷叶铁线蕨与铁线蕨随着干旱的加剧，总生物量呈降低的趋势；当处于同一水分含量时，随着光强的增加，总生物量呈增加的趋势，LAR一般随土壤含水量的增加而降低，随光强的增加而降低，当处于种内和种间竞争时候，荷叶铁线蕨的总生物量仍遵循这种规律，但是因为资源的抢夺造成总生物量明显降低。

This study will be mainly through potted lotus leaf fern Adiantum seedlings and wire division to transplant fern, and then through the different light intensity and moisture content of the orthogonal to quantitative research lotus leaf fern Adiantum optimum growing conditions, and through Transplant Research Laboratory test pilot of the two species, competition between species to find lotus leaf fern Adiantum way to protect endangered mechanism and provide a theoretical basis.

本研究主要通过盆栽将荷叶铁线蕨的幼苗与铁线蕨进行分株移植，再通过在不同光照强度和水分含量的正交实验来定量研究荷叶铁线蕨最适生长条件，并且通过实验室移栽试试验探究两物种之的种内，种间竞争关系为寻找荷叶铁线蕨濒危机理和保护途径提供理论依据。

Exploitations and prospects of natural lotus leaf bacteriostat were discussed.The main results were as follows:1、The extraction rates of crude extract, flavonoids, alkaloids and volatile oils from lotus leaves were high, and they had strong inhibition on bacteria activities but no distinct inhibition on yeast and mold.

主要研究结果如下：1、荷叶粗提物以及荷叶中的主要功能性成分黄酮、生物碱和挥发油都具有较高的提取得率，对所有供试细菌有较强的抑制作用，对霉菌和酵母菌的抑制作用相对较弱。

By identifying the obtained components by TLC, three alkaloids components were detected, among which one was nuciferine. The other two were N-Nornucirerine and O- Nornucirerine, identified by Nuclear Magnetic Resonance and Mass Spectrum.6、In order to exploit natural bacteriostat of lotus leaves, optimal extraction process and antibacterial characteristics were studied.

两者得到的化合物分别经TLC鉴别，得到3个生物碱成分，其中1个为荷叶碱，其余两个应用核磁共振和质谱等技术鉴定为N-去甲荷叶碱和0-去甲荷叶碱。6、从荷叶天然食品抑菌剂开发的角度，研究了荷叶抑菌有效成分的提取优化工艺及抑菌特性。

Antibacterial experiments results showed that extractions of bacteriostat of lotus leaves had significant antibacterial activities, and the antibacterial characteristics were stable, slightly affected by environment Conditions, such as pH value, temperature, concentration of cations. Also, the extractions exhibited strong antibacterial activities very soon.

抑菌试验结果表明，荷叶抑菌有效成分提取液具有较强的抑菌活性，且抑(来源：ABe064C论文网www.abclunwen.com)菌性能稳定，受环境条件，如pH值、温度、金属离子浓度等变化影响较小，且较短的作用时间就表现出较强的抑菌作用，因此，荷叶天然食品抑菌剂的开发具有很好的应用前景。

You are the big drop of dew under the lotus leaf, I am

露珠对湖水说道：你是在荷叶下面的大露珠，我是在荷叶上面

You are the big drop of dew under the lotus leaf, I am the s

露珠对湖水说道；你是在荷叶下面的大露珠，我是在荷叶上面的较小的露珠。

One on its upper side , said the dewdrop to the lake.

88露珠对湖水说道：你是在荷叶下面的大露珠，我是在荷叶上面的较小

The results showed: when TLC solvent system was: chloroform - methanol - diethyl amine = 90:9:1, alkaloids from lotus leaves were well isolated, and five components were isolated and identified by spraying Dragendorff reagent. The optimal conditions of HPLC solvent system were: methanol- water - diethyl amine = 75:25:0.0125. Under the conditions, components of alkaloids from lotus leaves were separated very well, and ideal RP-HPLC peaks were obtained.5、Methods of High Speed Counter Current Chromatography and preparative High Performance Liquid Chromatography applied to identify and purify alkaloids from lotus leaves were set up. When solvent system of HSCCC was: chloroform - methanol - water (pH=4.00)= 4:3:2, speed of chromatogram was 700rpm, flow speed of the mobile phase was 2mL/min, four pure components relatively were attained by isolation and purification.

结果表明：薄层层析溶剂系统为氯仿：甲醇：二乙胺=90：9：1，使荷叶中的生物碱达到了较好的分离效果，并用改良的碘化铋钾试剂喷雾显色，共分离鉴别出了5种荷叶生物碱；分析型HPLC分析检测的较优溶剂系统为甲醇：水：二乙胺=75：25：0.0125，使荷叶生物碱的各个组分达到了基线分离，并获得了较好的峰形。5、建立了高速逆流色谱和制备型HPLC技术分离纯化荷叶生物碱的具体方法。H(来源：ABC46论文网www.abclunwen.com)SCCC法在溶剂系统为氯仿：甲醇：pH4.00的水=4：3：2，色谱仪转速为700rpm，流动相流速为2mL／min的条件下，分离纯化得到了4个纯度较高的化合物。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。