药物处理

The group disposed by Acetylspiramycin doesn"t show parallel results; The strip gray scale of exotoxin A decreased gradually with the increasing dosages of Azithromycin and Roxithromycin, which doesn"t show in group disposed by Acetylspiramycin and group PAO-JP2; The strip gray scale of lasR, detected by RT-PCR, decreased gradually with the increasing dosages of Azithromycin, which doesnt show in group PAO-JP2.Conclusions: There were obvious differences between PAO1 and PAO-JP2 in biology characteristics in vitro.

本研究发现大环内酯类药物在生物被膜培养过程中分别处理PAO1、PAO-JP2，结果提示大环内酯类药物(14、15元环)通过对QS系统的影响可有效抑制铜绿假单胞菌生物被膜的形成；通过QS系统有效抑制铜绿假单胞菌的毒力因子的产生，16元环的大环内酯类药物乙酰螺旋霉未见上述结论；lasR基因的反转录结果提示阿奇霉素可在转录水平抑制QS系统发挥其作用。

Adding a few of co-solvent properly is favorable to SAA process in the experiments of water soluble drugs, and better results will be obtained. Considering solvent has significant effects on phase equilibrium in the saturator, ethanol is more suitable for the experiments of non-water soluble drugs.

处理水溶性药物时，适当地加入一些协溶剂有利于SAA过程的进行，并能得到更好的实验结果；处理非水溶性药物时，溶剂对混合器内的相平衡行为有重要的影响，乙醇更适合作为SAA过程的溶剂。

It is especially discussed that the principle, operation process and effects of the operation parameters on the characteristic of the products when the hydrophilic drug micronized with the supercritical assisted atomization which is an improvement on the supercritical anti-solvent technology. It is also introduced that the embedment of hydrophobic drug into biodegradable materials with the use of surfactant as well as the effect of different molecular weight of carrier and different ratio of complex carrier on the characteristic of products is made. It is pointed out that the use of surfactant will improve the micronization of microparticles of the hydrophobic drugs.

在药物微粉化方面，着重介绍了超临界辅助原子化法对水溶性药物的处理，包括该方法的原理、操作方法以及操作参数对产品性能的影响，证明该方法用于改善水溶性药物的微粉化具有良好的效果；在制备药物微球方面，重点介绍了不同操作条件和不同分子质量的载体、不同配比的复合载体对药物微球性能的影响以及表面活性剂在亲脂性及离子型药物微球制备中的应用，指出添加表面活性剂将更有利于得到该类药物的微球。

Serum containing SSTG was obtained at different time after perorally administrated with different dose of SSTG.The cardiomyocytes were deprived of oxygen and glucose to mimic hypoxia reoxygenation injury and were treated by serum containing SSTG when reoxygenation.LDH content in supernatant was detected after reoxygenation.LDH release suppression rate was used to study the time-effect and dose-effect of serum containing SSTG.

以中药有效组分配伍方剂双参通冠方不同灌胃剂量、药后不同取血时间所得的药物血清为受试药物；培养心肌细胞，进行缺氧复氧实验，复氧同时给予不同双参通冠方药物血清处理，实验结束后取培养上清检测LDH值，以LDH释放抑制率为指标，观察含药血清药理作用强度与体内给药的量效、时效关系。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果：经槲皮素处理的人结肠癌SW480细胞数量减少，部分细胞体积缩小，细胞膜完整，胞浆浓缩，核染色质固缩，细胞核碎裂，形成凋亡小体；MTT法检测显示当作用浓度为30μmol/L~90μmol/L时，槲皮素对人结肠癌SW480细胞的生长有抑制作用，其抑制作用随着作用浓度的增加和作用时间的延长而增强；流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期，大部分细胞被阻断于S期，随药物浓度的升高和作用时间的延长，G0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少；酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9，随浓度的升高，MMP-2及MMP-9的分泌量减少；免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后，Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

Thirdly ,under 25 0.5 ,pharmacocinetics and residues of orally administered at single and multiple dose of furazolidonum (60mg/kg) were investigated in grass crap , concentration of furazolidonum in Tissues were measured using HPLC and processed with MILLEMNIUM32 work station. The plasma concentration-time data was analyzed with MCPKP practical pharmacokinetics software.

其三，在25±0.5℃水温条件下，按60mg／kg的剂量给平均体重为65±10g的草鱼单次和多次灌服呋喃唑酮，用高效液相色谱法检测用药后不同时间血浆、肝脏、肌肉、肾脏中药物浓度，采用MILLEMNIUM~(32)工作站处理原始数据，然后用MCPKP药代动力学软件处理药时数据，对药物的吸收、分布、消除及组织残留进行研究。

Results: When incubated with different concentrations mitomycin for 24 hours, the body cells from 40μmol/L to 70μmol/L showed apoptosis under transmission electron microscope, chromatin condensation, even nuclear fragmentation, to form the apoptosis body can be seen.

体细胞经不同浓度的药物处理24h后，透射电镜下发现在40μmol/L～70μmol/L的各组细胞均有凋亡细胞，细胞核固缩、凝聚，甚至断裂，形成凋亡小体。

Methods: An animal model of pigment gallstones was established in male guineapigs by hypodermic injection of lincomycin.

1.3 动物分组、造模及药物处理实验豚鼠随机分为空白组、模型组、清胆胶囊组和养肝利胆颗粒组，每组各8只。

For GI contrast studies, this field would contain the pretest diet, e.g., low residue for two days, NPO before study, and the preferred purgatives.

每个单独的药物处理，饮食或准备应用一个重复分界符来分界。

It has streptomycin and dihydrostreptomycin crossing rate of 100 %, simple sample pre-treatment including water dilution of milk sample and extraction with buffering phosphoric acid solution and pH regulation of pork and kidney sample, fast detection, high detection sensitivity up to 11.2 microgram/L and other advantages, and may be used in detection of residual streptomycin and similar medicines in animal food products.

本发明的检测链霉素药物的酶联免疫试剂盒为多残留检测试剂盒，链霉素和双氢链霉素的交叉率为100％；对样品的前处理要求低，样品前处理过程简单，牛奶样品用水稀释后可直接测定，猪肉和肾脏经磷酸缓冲液抽提后调pH即可上样，并且能同时快速检测大批样品；测定方法简单，省时，牛奶样品在1小时之内可得出测定结果；最低检测限达11.2μg/L，具有高特异性、高灵敏度、高精确度、高准确度等特点，可在动物性食品链霉素药物残留检测中发挥重要作用。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新