药物依赖性

Objective To evaluate the effect of treatment on chronic insomnia in hypnotic-depengdent by propofol anesthetize induce sleeping.

目的探讨异丙酚麻醉诱导睡眠治疗药物依赖性慢性失眠症的临床疗效。

Methods With patient′s written informed consent,thirty chronic insomnia patients were cured by propofol anesthetize induce sleeping in our department.

方法选择本科收治的30例药物依赖性慢性失眠症患者，在患者自愿并征得其书面同意后给予麻醉诱导睡眠治疗。

Treated with high concentration of piperine （0.56 mmol/L） for 24 hours and 48 hours respectively, following a period of growth retardation, the cells of 24-hour-group could recover slowly while the 48-hour-group showed inconvertibility.

由此认为：胡椒碱对C6/36细胞株有毒性作用，但药物诱导的细胞凋亡在细胞毒性作用机制中不占主导地位；胡椒碱对C6/36细胞的作用有时间依赖性，延长作用时间，药物对细胞的毒性作用增强。

Results showed that L-THP possessed negative inotropic and negative chronotropic action, lowed left ventricular pressure and it's infinitesimal calculus, decreased heart output in per min., shorten 〓 and 〓 of action potential of ventricular papillary muscle of guinea pig and even the plate-phase of action potential disappeared in larger dose group , but prolonged 〓. we studied transmembrane transportation of 〓 after administering L-THP , L-THP not only depressed uptake and increased release of Ca 2+ in normal myocardium, but also it showed stronger inhibition in cell injured by ISO, and fully eliminated the effect of ISO increasing uptake and decreasing release of 〓. By detecting the 〓 content of ventricular single myocyte using fluorescent probe Fluo-3/AM, results correspond to that done above, L-THP decreased 〓 content inside of cell significantly. Using whole-cell mode, we observed the effect of L-THP on Ica in ventricular single cell, it was found that L-THP decreased Ica, the effect increased with increasing it's dose, and had voltage, frequency-dependency. Dose highly corelated to effect, corelative constant is 0.87. When washing cell with normal tyre's solution, Ica could recover, which proved the blocked action of L-THP on calcium channel once again. Meanwhile, L-THP reduced production of MDA in heart injured by ISO in dose-dependent manner, improved content of GSH-Px, in case tissue or cell free from free radical damage.

结果显示，该药物具有负性肌力和负性频率作用，降低左室压及其微分，终使每分心输出量减少；缩短豚鼠心室乳头肌动作电位时程的〓和APD〓，大剂量使动作电位平台消失，〓延长；同位素示踪法研究证实L-THP抑制〓摄取，促进〓释放，降低细胞内的〓含量；单通道水平的研究证实，L-THP减少豚鼠心室肌单细胞内向钙电流，且具有剂量，电压，频率依赖性，其作用易于洗脱，药物剂量与Ica也具有高度相关性（相关系数r为-0.87）；L-THP还可抑制ISO损伤心肌组织所致的丙二醛含量增加，增加谷胱甘肽过氧化物酶的量，使组织细胞免受损伤。L-THP阻断L-型〓通道，抑制〓的摄取，促进〓的释放，降低细胞内〓含量L-THP作用的离子基础。

A significant change is the panel's use of the term persistent rather than chronic pain, which connotes negative stereotypes of malingering, futility in treatment, or drug-seeking behavior.

最大的变化是专家小组将慢性疼痛变成了顽固性疼痛，这意味著否定了装病、无意义的治疗或药物依赖性的行为。

American scientists have found that piperine is a kind of nerve drug, which can't cause drug dependence and has strong effect on addiction treatment and pain relief.

经美国科学家研究发现，胡椒碱是一种不产生药物依赖性的神经类药物，具有强大的戒毒和镇痛效果。

It is important to note that minoxidil-stimulated regrowth of terminal hairs is dose-dependent.

需要注意的是，米诺地尔刺激终毛重新生长是有药物依赖性的。

And then,the soluble target protein was purified with Ni-NTA column,and its C-terminal was sequenced with mass spectrum analysis.13 amino acids of C-terminal is identical to the Plasmodium falciparum TCTP when blasted in GenBank. Plasmodium falciparum ; Translationally controlled tumor protein ; Cloning expressing

虽然报道认为TCTP具有钙结合、金属内环境稳定、细胞内信号传导、作为IgE依赖性组胺释放因子、作为青蒿素类药物受体蛋白(Bhisutthibhanet al。，1998)和在约氏恶性疟原虫青蒿类药物抗性系中过表达等功能，但其具体生物学功能尚待进一步研究阐明

Results: The growth of NB4 cells was inhibited significantly after treated with berbamine at different concentrations for different time points, the IC50 value was 3.860μg/ml at 48 hours. Morphology analysis showed the characteristics of apoptosis, and the DNA agarose electrophoresis showed the typical DNA ladder. The apoptosis rate increased from 2.83% to 58.44% after treated with berbamine at 12μg/ml for 18 hours. The expression of PML-RARα mRNA presented no significant changes, however, Survivin mRNA was decreased dramatically.

结果：经不同浓度小檗胺处理不同时间后，NB4细胞生长出现明显的抑制，并呈现明显的时间剂量依赖性，48h IC50为3.860μg/ml；细胞形态学观察可见细胞凋亡现象，DNA琼脂糖电泳显示典型的凋亡梯形图：流式细胞仪检测发现细胞凋亡率明显增加，经48h作用后，凋亡率从2.83%增至12μg/ml时的58.44%；虽未发现药物作用后PML/RARα基因表达量的改变，但检测到NB4细胞经药物作用后Survivin基因表达量的减少和Caspase3表达的增加，Caspse3阳性率从对照组的2.06%增至12μg/ml时的70.89%。

A pH-responsive controlled releasing carrier for medicine is a chitosan-base gel which is prepared from Chitosan, gelatin and pectin through cross-linking by aldehyde cross-linking agents, and features obvious pH dependency, high biocompatibility and abling to biologically degradate. It can be used for detention-type controlled releasing medicines to avoid toxic' by-effect due to excess dosage.

一种pH响应性药物控释载体，采用壳聚糖、明胶和果胶等天然高分子材料用醛类交联剂共交联合成了壳聚糖基凝胶，用该凝胶制备的药物释放体系呈明显的pH依赖性，用于口服抗酸、治疗胃肠疾患的滞留型控释制剂，可避免释放药量过度导致的毒副作用，且该控释载体生物相容性好、可生物降解。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。