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Objective To study the effects of different solvents extraction to the alkaloid content of Radix Tinosporae.

目的 研究不同提取方法对金果榄提取物中盐酸药根碱与盐酸巴马汀含量的影响。

By synthetically analysizing the physical and chemical properties of all components and particle size, the content of F2641 in the JOB-iC is determined by gravimetric analysis after F2641 is seperated from HMX,TATB and PNP through ? alkali reflux. Determination conditions is set as follows the sample is boilingly refluxed for six hour in a constant temperature bath after adding lOOm! 8.0 ?? 0.1% NaOH. The solvent DMF saturated by TATB is used for extracting HMX,F21 and PNP from the sample and TATB is seperated by crucible filter G4. The mass precent of TATB is determined by extraction fractionation. The mass percent of PNP is measured by multiwavlength linear regression ultraviolet spectrophotometry. The testing conditions are set as follows:multiwavlength constitution: X=267nxn, 275nm, 283nm, 29mm, 299nm,the application scope of the Lambert-Beer law: the concentration of PNP is O.005?0.O25mgIml, the concentration of HMX is 0.060.3Omg/ml,absorption coefficents of PNP and HMX are solved by the slope of linear regression curve of absorbency- concentration of standard solution of PNP and HMIX for measuring wavelengthes,on the basis of the Lanibert-Beer law and absorbancy additivity principle, the slope of linear regression curve of A/E(1) and E1 of PNP and HMX solution is regarded as the concentration of PNP in the solution.

根据传爆药中各组分的物理、化学性质及主体炸药的粒度大小,进行综合分析,确定了采用碱回流重量法测定JOB-1C 中F_(2641)的含量,测定条件:加入100ml浓度为8.0±0.1%的氢氧化钠,在恒温水浴中煮沸回流6h;采用溶剂萃取法测定JOB-1C中TATB的含量,选择TATB饱和的二甲基甲酰胺为萃取溶剂,用G4坩埚式过虑器进行萃取分离;采用多波长线性回归紫外分光光度法测定JOB-1C中PNP的含量,通过实验确定了多波长组合:λ=267nm,275nm,283nm,291nm,299nm;朗波—比耳定律的适用范围为PNP浓度:0.005~0.025mg/ml,HMX浓度:0.06~0.30mg/ml;在测定波长下,对PNP、HMX标准溶液的吸光度—浓度进行线性回归,由回归曲线的斜率得出PNP、HMX的吸收系数;根据朗波—比耳定律和吸光度加和性原理,在测定波长下,对PNP、HMX 两组分混合溶液A_i/E_HMX(i与E_PNP(i/E_HMX(i进行线性回归,回归曲线的斜率即为混合溶液中PNP的浓度。

Effects of temperature and mobile phase composition on the interaction betweenβ_2-AR and both of palmatine and jatrorrhizine.

3研究了温度及流动相组成对盐酸巴马汀和盐酸药根碱与β_2-AR之间相互作用的影响。

RESULTS: The results showed that the water extract of phellodendron and one of its main components increased the cell membrane fluidity in the inactive state, but the other two components, berberine and jatrorrhizine, decreased the cell membrane fluidity.

结果:在无ConA刺激时,黄柏的水提物可提高小鼠脾细胞膜的流动性,6.25%的浓度与对照组相比有极显著性差异;小檗碱与药根碱可降低脾细胞膜的流动性,与对照组相比均有显著性差异;巴马汀可提高脾细胞膜的流动性,与对照组相比有极显著性差异。

RESULTS: The results showed that the water extract of phellodendron and one of itsmain components increased the cell membrane fluidity in the inactive state, but the other two components, berberineand jatrorrhizine, decreased the cell membrane fluidity.

结果:在无ConA刺激时,黄柏的水提物可提高小鼠脾细胞膜的流动性,6.25%的浓度与对照组相比有极显著性差异;小檗碱与药根碱可降低脾细胞膜的流动性,与对照组相比均有显著性差异;巴马汀可提高脾细胞膜的流动性,与对照组相比有极显著性差异。

UV was applied to determine the concentration of total alkaloids and total flavones; Simultaneous determination of berberine, jatrorrhizine, palmatine, baicalin and geniposide by HPLC; The solid holdup and precipitation rate were calculated by the formula.

采用UV测定其中总生物碱、总黄酮的量, HPLC同时测定小檗碱、药根碱、巴马汀、黄芩苷、栀子苷的量;计算固含率,沉淀率。

A quick and nondestructive method using near infrared diffuse reflectance spectroscopy was presented to determine the contents of berberine, palmatine, jatrorrhizine and total alkaloid of Coptis extract.

采用近红外漫反射光谱法对黄连浸膏粉中小檗碱、巴马亭、药根碱和总生物碱含量进行快速无损检测。

The research results will conduce to the exploration of Mahonia plants.3^ In the experiment, we induced callus with the M.eurybracteata.

用HPLC法对收集到的材料茎中三种主要生物碱(小檗碱、药根碱、巴马汀)的含量进行了分析。

AIM: To investigate the effect of phellodendron and three kinds of its main components, which have asuppressive effect on the immune system, on the membrane fluidity of normal murine splenocytes.

目的:观察具有免疫抑制作用的药物黄柏及其3种主要成分:小檗碱、药根碱与巴马汀对正常小鼠脾细胞膜流动性的影响。

AIM: To investigate the effect of phellodendron and three kinds of its main components, which have a suppressive effect on the immune system, on the membrane fluidity of normal murine splenocytes.

目的:观察具有免疫抑制作用的药物黄柏及其3种主要成分:小檗碱、药根碱与巴马汀对正常小鼠脾细胞膜流动性的影响。

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