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药根碱

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On the basis of all the experimental results and the molecular modelling study, it is considered that Jatrorrhizine and Berberine binds to the site I and site II mainly by hydrophobic interaction and there are hydrogen bond and electrostatic interactions.

荧光竞争实验以及分子对接研究表明药根碱和小檗碱的结合区域分别位于蛋白质的亚结构域ⅡA和亚结构域ⅢA中,主要通过疏水作用结合,但还包括静电作用力、氢键。

Objective To study the hypoglycemic activity of jatrorrhizine and compare the effects of Jat, berberine, Huanglian Decoction, and compounds-mimic prescription on blood glucose level in mice.

目的研究药根碱的降血糖作用,并比较Jat、小檗碱、黄连煎剂及模拟方对小鼠血糖水平的影响。

Both the BER and the JAT is can be absorbed in whole intestine and the results indicated that the absorption of BER and JAT compiled with the first order kinetics through passive diffusion mechanism.

盐酸小檗碱和盐酸药根碱在大鼠肠道中均有吸收,其吸收呈一级动力学过程,吸收机制为被动扩散。

Result: Glutin could make the absorption of jatrorrhizine, coptisine, berberine and total alkaloids increased by 30%. Tween 80 and arabic gum did not affect the absorption of berberine, but inhibit that of other alkaloids. There had no influence of lecithin on the absorption of alkaloids.

结果:明胶使药根碱、黄连碱、小檗碱和总生物碱的吸收均提高约30%;聚山梨醇酯和阿拉伯树胶对小檗碱的吸收无影响,而抑制其他3种生物碱和总碱的吸收;卵磷脂几乎不影响各生物碱的吸收。

Chapter 2: The binding of Jatrorrhizine and Berberine with human serum albumin was investigated by spectroscopy and the molecular modelling study.

第二章:应用光谱法结合计算机模拟技术对人血清白蛋白与异喹啉类生物碱药根碱、小檗碱的相互作用进行了详细的研究。

Effect of jatrorrhizine, berberine, Huanglian Decoction and compound-mimic prescription on blood glucose in mice

药根碱、小檗碱、黄连煎剂及模拟方对小鼠血糖的影响。

The studied results indicated that Jatrorrhizine and Berberine can strongly bind to human serum albumin. The binding constant were 7.278×10~4 M~(-1) and 4.071×10~4 M~(-1) at 296K.

研究结果表明,人血清白蛋白与药根碱、小檗碱之间可以发生相互作用,而且有较强的结合,在296K时结合常数分别为7.278×10~4M~(-1)与4.071×10~4M~(-1)。

Moreover, the water extract and the alkaloids of Rhizoma Coptidis, as well as berberine, palmatine and jatrorrhizine could increase the EFSinduced contraction.

为了验证我们的这种推测,本文研究黄连水提物、黄连总生物碱以及黄连中的主要化学成分小檗碱、巴马丁碱和药根碱对豚鼠胃窦环行肌收缩的影响。

A method for the simultaneous determination of berberine, palmatine, jatrorrhizine, magnoflorine and coptisine from Rhizoma Coptidis samples based on the nonaqueous capillary electrophoresis mode has been developed.

建立了一种非水毛细管电泳同时测定黄连饮片生品与炮制品中小檗碱、巴马汀、药根碱、木兰碱和黄连碱含量的方法。

These results indicated that palmatine had one common binding site on the immobilizedβ_2-AR column,the association constant was 2.93×10~4 L/mol at pH 7.2 and 25℃,while jatrorrhizine had two bingding sites,the association constants at these two sites were 1.93×10~4 and 1.56×10~5 L/mol,respectively.And the molar ratio of weak bingding sites to strong binding sites was 93:7.Palmatine and jatrorrhizine had competed for low-affinity site in immobilizedβ_2-AR.

结果表明,盐酸巴马汀与β_2-AR只有一类结合位点,在流动相pH 7.2,温度25℃时盐酸巴马汀与β_2-AR的结合常数为2.93×10~4 L/mol;盐酸药根碱与β_2-AR有两类结合位点,在这两类结合位点上的结合常数分别为1.93×10~4和1.56x10~5L/mol,两类结合位点数的比值为93:7,且盐酸巴马汀和盐酸药根碱竞争低亲和力的结合位点。

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