药

The invention relates to the isolation and preparation of an active fraction from plant Cuminum cyminum, its further purification and standardization as chemically defined entity with their intended use as drug bioavailability enhancer for the drugs belonging to therapeutic categories such as antimicrobial, antifungal, anti-viral, antitubercular, antileprosy, anti-inflammatory, antiarthritic, cardiovascular, antihistaminics, respiratory distress relieving drugs, immunosuppressants, anti-ulcerogenic, anti-cancer, CNS drugs, corticosteroids, nutraceuticals in compositions to be administered orally/parenterally, topically, inhalations, rectally, vaginally in human beings and/or veterinary conditions.

本发明涉及植物孜然芹中的一种活性级分的分离和制备，进一步的纯化以及它作为化学确定实体的标准化，和它作为治疗类药物的生物利用率增强剂的特定用途，这类药物包括如抗微生物剂、抗真菌剂、抗病毒剂、抗结核药、抗麻风病药、抗炎药、抗关节炎药、心血管类药、抗组胺药、呼吸窘迫缓解药、免疫抑制剂、抗溃疡药、抗癌药、CNS药、皮质甾类药、营养制品，它们以组合物的形式通过口服/肠胃外，局部，吸入直肠，阴道施用于人和/或兽医学病症中。

OBJECTIVE To design and develop a clinical pharmacokinetics and individual drug dosage regimens system CPKDP program for estimating individual pharmacokinetic parameters and optimizing individualizing dosage regimens using routine clinical data of plasma levels. METHODS According to the principle of population pharmacokinetics and Bayes" formula, CPKDP program was written with FOXPRO 2.6 and BORLAND C++ 3.1, and was run on 486 microcomputer with UCDOS 5.0. RESULTS CPKDP program was adapted for drugs with different characteristics of pharmacokinetics, and for single dose or multiple dose administration. Using 1 or 2 measured drug concentrations, individual patient"s pharmacokinetic parameters were estimated based on population-average pharm-acokinetic parameters. CONCLUSION CPKDP program is applied for estimating pharmacokinetic parameters and optimizing drug therapy in individual patients.

目的为利用临床常规监测的血药浓度数据估算病人的个体药动学参数，优化给药方案，设计、研制了临床药动学给药个体化系统CPKDP程序方法依据群体药动学原理及Bayes公式，CPKDP程序用FOXPRO 2.6和BORLAND C++ 3.1开发，在希望汉字系统UCDOS 5.0平台上运行结果CPKDP程序适用于具有不同药动学特征的多种药物，适用于不同给药途径的单剂量或多剂量给药在群体药动学参数的基础上，结合病人个体特征，以1，2个血药浓度作为反馈，即可拟合估算个体药动学参数，优化给药方案结论经初步应用，CPKDP程序是应用Bayes反馈法开展临床个体化给药工作非常实用的工具

Light output analysis tests character of spectrum radiate of pyrotechnic mixtures and analyses wave band distribute of light by comparing different static blaze spectrograms of the pyrotechnic mixtures. Screen out the better pyrotechnic mixtures according to request of pumping source. Press experiments in airtight erupt container experiment, which simulate the circumstance that pyrotechnic mixtures deflagrate in quartz pipe, realize the press influence to the quartz pipe wall and the disciplinarian of axes press variety. Apply these pyrotechnic mixtures to Nd:YAG lasers as the pumping source and compare pumping efficiency of different pyrotechnic mixtures. Pumping efficiency is an important standard which checkout if these pyrotechnic mixtures are the better pumping source. Sensitivity experiment analysis incarnate security of pyrotechnic mixtures that is the essential parameter in the process of design, manufacture and use.

通过对烟火药的热分析(爆热分析、DTA、TG)，了解备选烟火药反应过程中的能量输出特性及反应过程中的物理、化学变化规律；光输出分析实验对烟火药配方光谱辐射特征进行测试，比较其静态火焰光谱图，分析不同烟火药剂光辐射波段分布特征，根据激光泵浦源的辐射要求，进而筛选更符合激光泵浦源药剂要求的烟火药；模拟烟火药在石英管中爆燃环境的密闭爆发器压力分析实验反映了烟火药在石英管中反应时对管壁的压力影响以及管内轴向压力变化规律；将烟火药作为泵浦源应用于Nd：YAG固体激光器，比较不同烟火药的泵浦效率，作为检验烟火药是否适合作为激光泵浦源的重要标准；感度实验分析体现了各种烟火药的安全性，是烟火药设计、制造和使用的必要示性数。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The first day of fever 38.5, burning吃了药receded, and then there are some minor coughing, sneezing, runny nose, medicine and Zailin Cephalosporium, as well as the town of cough medicine, to the fifth day of fever 38.5 morning again, to pull吃了药now fine, but there are some slight cough.

第一天开始发烧38.5，吃了药烧退了，接着就有一些轻微的咳嗽，打喷嚏，流鼻涕，吃药头孢和再林，还有镇咳的药，到第五天早上又发烧38.5，吃了药又退了，现在也蛮好，只是有一些轻微的咳嗽。

Where are you you, I am in Beijing, baby in my home three and a half months when diarrhea, there are seven or eight times a day, go to the hospital they said La dysentery, to open the medicine, good food to eat for three days later went Children's Hospital, the doctor also said to be eating dirt,药吃opened the next day just fine.

你在哪呀，我在北京，我家宝宝在三个半月的时候也拉肚子，一天也有七八次，去医院人家说是拉痢疾，给开了药，吃了三天没吃好后来就去了儿童医院，大夫也说是吃了脏东西，开了药吃了第二天就好了。

Institute on Drug细末total, each with a little tune药末Add egg into a thin paste, before晚睡face with warm water bath, and then cream applied to this spot, the morning wash, facial attending deringing .

上药共研细末，每次用少许药末放入鸡蛋清调成稀膏，晚睡前先用温水浴面，然后将此膏涂于斑处，晨起洗净，主治面部色斑。

Mr. I and plans for half a year want a BB but have not been pregnant this month to the hospital for an examination has been found pregnant as a result of six months but were not pregnant so there is no care to protect their early physical illness is as follows吃了药specific menstruation is the last time I was on the 4th to January 2 (can not recall the specific date that the last two days anyway) cycle for 29-32 days as a result of Kim Jong-January 16 Doctor hospital was opened two days of ofloxacin吊针two bottles a day and oral inflammation hundred net service for three days or not some of the symptoms also had oral Lowe four days after 28 No. Because when cooking large half fingernails cut off after two days due to inflammation of eating cefradine BB always wanted as a result of another failed as Friend introduction has been gradually taking ginseng cream until February 3鹿胎feeling some discomfort to the hospital post-mortem examination urine was very weakly positive on the drug problem at that time ask the director of hospital doctors when she helped me just as long as the operator told me to eat before February 5 in medicine are not all right to ask whether she was in the comfort me?

我和先生计划了半年想要个BB可是一直没有怀上这个月去医院检查验出已怀孕了可是由于大半年都没有怀上所以就没有小心保护自己身体早期因病吃了药具体情形如下我最后一次月经是一月二号还是四号来(具体日期记不起了反正就是这两天)周期为29至32天一月十六号由于泌感到医院看病医生开了两天的氧氟沙星吊针每天两瓶及口服药百炎净服了三天未还是有些症状又口服了洛威四天之后二十八号由于做饭时切掉大半块手指甲后由于发炎吃了两天的头孢拉定另外由于一直想要BB未果经朋友介绍一直在陆续服用人参鹿胎膏直到二月三号感觉有些不适去医院检查验尿呈极弱阳性当时就服药问题问了医院的主任医生当时她帮我算了算告诉我只要在二月五号前吃的药都没什么大碍的请问她是否在安慰我呢？

I have seven months of a baby, I have been for him to eat Di Qiao calcium, drink a three sperm喂药harder than calcium gluconate, or like to eat Di Coincidentally, a half tablets a day, beaten at the end of spoon, with chopsticks licking eat sticky baby!

我孩子也七个月了，我一直给他吃迪巧补钙，喝过一次三精葡萄糖酸钙比喂药还难，还是喜欢吃迪巧，每天半片，用勺子碾成末，用筷子粘孩子舔着吃！

Intermittent self-treatment drug汪经理busy with high blood pressure, symptoms usually clear, and on the buy back on a pile of药吃a few days, symptoms of light, and put medicine aside.

间断治疗自行停药忙碌的汪经理患有高血压，平时症状明显了，就买回一堆药吃上几天，症状轻了，就把药扔在一边。

By using competitive adsorption of activated carbom to remove unfavorable influence of carbon in carbonaceous gold concentrate on leaching process, the leaching rate of gold concentrate can be improved to 88% as compared with 10%by direct cyanidation.

本文论述了利用活性炭竞争吸附作用，消除含炭金精矿中炭在浸出过程中的影响，使金精矿的浸出率可由直接氰化的10％提高到88％。

The only thing you're ever gonna be king of is King of the Stupids!

你只配做傻瓜的国王！