荧光黄

Purpose To study the characteristics of fluorescence of emodin and its effects on fluorescence analysis in smooth muscle cell.

目的 观察大黄素的荧光光谱特性，以探讨其在平滑肌细胞中干预后对荧光分析的影响。

The interaction between three small flavoind drug molecules, which are apigenin, galangin and genistein, and BSA is investigated with fluorescence spectroscopy using the drug's quenching on the endogenous fluorescence of serum albumin.

采用荧光光谱法，利用药物对牛血清白蛋白自身内源荧光的猝灭，研究了芹菜素、高良姜素和染料木素这三种黄酮药物小分子与牛血清白蛋白分子间的相互作用，研究发现药物分子与牛血清白蛋白分子之间发生了有效的静态猝灭。。。

Strains M110 and M113 isolated from Aedes albopictus and Armigeres durhami, respectively, which were fatal to suckling mice at 4 days after intracerebrally inoculation and had cytopathologic effects on BHK21 cells, showed a positive reaction to dengue antibody and could be amplified Ly using flavivirus primers and dengue type-4 NS1 gene primers and dengue type-4 NS2a gene primers.

结果 从白纹伊蚊和达勒姆阿蚊中各分离到一株病毒，分别命名为M110和M113，这两株病毒对BHK21细胞能产生明显的细胞病变，脑内接种乳鼠4d左右导致乳鼠死亡。该病毒经血凝、血凝抑制和间接免疫荧光试验提示为黄病毒。分别用黄病毒属特异引物、登革4型病毒NS1和NS2a基因片段特异引物进行RT-PCR扩增、序列测定，证实为登革4型病毒。

Aflatoxins were enhanced by mercury chloride solvent online and detected by fluorescence detector.

采用免疫亲和方法进行样品前处理，用甲醇－乙腈－水三元流动相体系分离黄曲霉毒素，氯化汞溶液在线衍生，荧光检测器检测，建立了新型的高效液相色谱柱后衍生测定黄曲霉毒素(B1、B2、G1、G2、M1)的方法。

The secondary structure compositions of free HSA and its Rhein complexes were estimated by the FT-IR spectra.

荧光光谱和同步荧光说明蛋白质与大黄酸的结合改变了蛋白质的微环境。

A series of study methods which can not research in this field begin to exert important affection. On the basis of the previous research, the following major innovative work was carried out in this dissertation.(1) Several methods including fluorescence spectra, CD and FT-IR specta methods were used to study the interaction of isoquinoline alkaloid、isoflavones and Rhein with serum albumin, and the results from every method was in good agreement;(2) The binding constants、the numbers of binding site、the binding region and the interaction force involving in the interaction of drugs and serum albumin were confirmed by Scatchard equation and Vant Hoff equation.

基于研究生物大分子与小分子相互作用具有极其重要的意义，本博士论文在前人工作的基础上，主要进行了以下几方面的创新性研究：(1)利用荧光光谱法、圆二色谱法、红外光谱法以及分子模拟等多种方法相结合研究了异喹啉类生物碱，异黄酮类化合物以及大黄酸与人血清白蛋白的相互作用，获得了准确一致的信息；(2)根据Scatchard方程和Vant Hoff方程详细研究计算了异喹啉类生物碱以及大黄酸与蛋白质结合机理包括结合常数、结合位点数、结合区域、结合力。

The accumulation of fluorogen may play a major role in the yellowing and increasing fluorescence of the lens with age.

荧光发生素在晶状体中积累，可能主要参与老化过程中晶状体变黄和荧光色素增加。

The binding of bis-ANS bound to blue membrane at different temperature are only one binding sites. 4. The research results of fluorescence, absorption spectra and stopped flow method used to track the process of transition from acidifying purple membrane to blue membrane indicate that the transition of the characteristic absorption band of retinal(from 570 to 605 nm) and the maximum excitation wavelengths of bacteriorhodopsin(from 326 to 331 nm) occurres at a narrow concentration range of HCl(11.5-13.3 mmol/L). The transition process performs within 0 to 2s.

通过荧光光谱、吸收光谱和停流技术对酸化紫膜转变成蓝膜变化过程的跟踪研究表明，视黄醛特征峰的递变(570→605nm)以及蛋白质荧光最大发射波长的递变(326→331nm)都是在狭窄的HCl浓度范围(11.5-13.3mmol／L)内同步进行的，该转变过程在0-2s之内极快完成。

Three kinds of fluorescence polymer with the structure of acridine yellow were synthesized and the sensor for measuring nucleic acid was also fabricated.

论文第三部分开展了以荧光高分子为敏感试剂的新型光化学传感器载体研究，合成了N，N-二甲氨基对苯甲醛缩醛类聚合物，考察了苯环上不同取代基对荧光性能的影响，研究了该类敏感膜对乳酸、尿嘧啶、胸腺嘧啶和腺嘌呤的响应性能；合成了三种具有吖啶黄结构的荧光高分子，制成了测定核酸的传感器。

The fluorescence spectra of Eu~(3+) may be used to study the structure ofcompounds.The band shift of Nd~(3+) in its absorption spectra,the red-to-orangeintensity ratio of the Eu~(3+) emission(~5D_0→~7F_2)/(~5D_0→~7F_1)and the yellow-to-blue intensity ratio of the Dy~(3+) emission~4F_(9/2→~6H_(13/2)/~4F(9/2→~6H(15/2)intheir fluorescence spectra may be used to study the degree of covalency of theLn-O bond and its relation with the electronegativity of M in Ln-O-M.

本文介绍了利用 Eu~(3+)的荧光光谱研究化合物的结构；利用 Nd~(3+)的吸收光谱的谱带位移和 Eu~(3+)的荧光光谱的红橙发射强度比(~5D_0→~7F_2)/(~5D_0→~7F_1)和 Dy~(3+)的黄兰比~4F_(9/2→~6H_(13/2)/~4F_(9/2→~6H_(15/2)，了解Ln—O—M中 Ln—O 化学键的共价程度以及 M 的电负性对共价程度的关系；利用吸收光谱、荧光光谱和磁化率研究稀土的价态以及利用磁化率研究化合物的组成。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。